Lecture 2 - Dx Stains Flashcards
Fluorescein solubility? absorption? emission? tear film color? Seidel's sign? metabolism?
- water soluble
- 493 nm (Cobalt blue) vs. 465 nm in blood
- 520 nm (yellow-green) emission
- Orange-yellow-green in tear film
- Seidel’s sign: fluorescent green in Bowman’s membrane or aqueous humor
- Hepatic metabolism
What is most likely to lead to false results for fluorescein?
tetracaine
What are some indications for fluorescein?
- foreign body
- abrasion or ulcer & edema
- alkaline burn: non-soluble particles
- dry eye staining & tear breakup time (TBUT)
What does HSV keratitis look like?
Simplex: notice it has a bulb like ends at the fern-like filament. Comes out as bright green.
What would herpes zoster look like?
a narrow and thin bright green
RGP contact lens adaptation is an indication for what?
Fluorescein
What is used to dx tear duct obstruction/epiphora via the Jones Test?
Fluorescein
For dx filamentous keratitis?
Fluorescein
Quenching (aka fluorescein with an anesthetic) causes an _______________ of IOP evaluation with application tonometry.
UNDER-ESTIMATION
Which dye is used for angiography (5ml 10% or 3ml 25% IV or 0.5% PO)?
Fluorescein
This genetic defect is manifest in the visual phototransduction cycle. The ATP-binding cassette transporter is defective and leads to a buildup of the toxic metabolite lipofuscin. It is a juvenile macular degradation that develops between the ages of 6-12 years.
Stargardt’s disease
Name the stain: absorption is 545-490nm stains mucus or devitalized tissue conjunctival and corneal application viewed under white light or red-free (aka green filter)
rose bengal
Name this stain:
mild antiviral properties
photo reactive: RBC hemolysis
rose bengal
Rose Bengal is a iodine-based derivative of _________.
fluorescein
Which stain for the following indications?
keratoconjunctivitis sicca (KCS)
superior limbis keratoconjunctivitis (SLK)
herpes simplex/zoster
argon laser photocoagulation
metastatic melanoma
rose bengal