Lecture #17 Flashcards
RNAs have ______ folding, and the _____ functions depending on the folding.
specific, diverse
RNA folding is driven by the formation of regions having _______ base pairs
complementary
Base paired regions form double stranded stems connected by _____
loops
Transcription enzymes - in both prokaryotic and eukaryotic cells are called DNA‐dependent RNA _________
polymerases
Incorporate nucleotides into an RNA strand complementary to the ____ template strand
DNA
RNA polymerase moves in a ___ to ___ direction; the DNA is unwound, and the polymerase assembles a complementary strand of RNA at 20–50 nucleotides molecule per second
3’, 5’
The frequency at which a gene is transcribed is tightly ______ and ________ function observed
regulated, proofreading
_______ have no nuclei
Bacteria
In bacteria, there is no physical ________ of DNA/RNA and ribosomes
separation
In bacteria, there is translation of the mRNA while its _______ is in progress.
transcription
Genes are organized in clusters that are transcribed together as a single mRNA molecule encoding for multiple proteins. These clusters are called ________
operons
Bacteria have 1 RNA _______, which is composed of __ subunits tightly associated to form a core enzyme.
polymerase, 5
_______: a region of DNA upstream of a gene where relevant proteins bind to initiate transcription of that gene.
Promoter
__ factor binds promoter
σ
Once about 10 nucleotides have been incorporated, the enzyme undergoes a _________ change. σ factor is loss. It becomes a transcriptional elongation _______
conformational, complex
The polymerase stops transcription when it reaches a __________ sequence and releases the completed RNA
terminator
In the absence of the sigma (σ) factor, the _____ _______ cannot interact with the DNA at specific initiation sites
core enzyme
_________ cells have nuclei
Eukaryotic
In ________ cells, there is physical separation of DNA/RNA and ribosomes.
eukaryotic
In eukaryotic cells, transcription and RNA processing steps happens in the _______
nucleus
In eukaryotic cells, the mRNA needs to be exported to the ________.
cytoplasm
In eukaryotic cells, translation happens in the ________
cytoplasm
There are _____ types of RNA polymerases in eukaryotes
three
Most rRNAs are transcribed by RNA polymerase ___
I
mRNAs are transcribed by RNA polymerase ___
II
tRNAs are transcribed by RNA polymerase ___
III
RNA polymerases have been characterized from each of the three domains of life: ?
archaea, bacteria, and eukaryotes
The ______ and eukaryotic enzymes are more similar in structure to one another than are the ______ and archaeal enzymes
archaeal, bacterial
What are the 3 transcription steps?
- Initiation
- Elongation
- Termination
- Transcription bubble around 35nt of _______-stranded DNA (melted DNA)
- Polymerase incorporates nucleotides (nt) into transcript in __-__ direction.
- About 9 nt DNA-RNA transient ______.
- Generation of ________ (positively supercoiled) DNA ahead and _______ (negatively supercoiled) DNA behind.
single
5’, 3’
hybrid
overwound, underwound