Lecture 13 Quiz

Question 1

According to Beer’s law, the amount of light ___ by a solution is ___ proportional to the ___ of a solution.

(a) transmitted; directly; concentration
(b) transmitted; inversely; concentration
(c) absorbed; directly; concentration
(d) absorbed; inversely; concentration

Answer 1

(c) absorbed; directly; concentration

Question 2

Which wavelengths describe visible light?

(a) 260 - 600 nm
(b) 500 - 900 nm
(c) 380 - 750 nm
(d) 400 - 940 nm

Answer 2

(c) 380 - 750 nm

Question 3

The analytical method based on detection of the portion of light scattered by the particles in a solution toward a detector not in the direct path of the transmitted light is known as:

(a) Turbidimetry
(b) Absorbance Spectrophotometry
(c) Refractometry
(d) Nephelometry

Answer 3

(d) Nephelometry

Question 4

The component of the spectrophotometer that isolates radiant energy of a desired wavelength while excluding other unwanted wavelengths is known as the:

(a) Monochromator
(b) Light Source
(c) Cuvette
(d) Photodetector

Answer 4

(a) Monochromator

Question 5

Light travels in:

(a) Planes
(b) Waves
(c) Flashes
(d) Lasers

Answer 5

(b) Waves

Question 6

If the color of an object is red, the other colors of the energy spectrum (orange, yellow, green, blue, purple, etc.) are:

(a) Absorbed
(b) Deflected
(c) Expressed
(d) Transmitted

Answer 6

(a) Absorbed

Question 7

If the absorbance of a patients’ sample added to total protein reagent is to be measured using a spectrophotometer, the blank should be comprised of:

(a) Patient Sample
(b) DI H2O
(c) Total Protein Reagent
(d) Patient Sample and Total Protein Reagent

Answer 7

(c) Total Protein Reagent

Question 8

In regards to Plank’s equation, what do E and γ represent, and how do they relate to each other?

(a) E = Energy, γ = Frequency; Directly Proportional
(b) E = Energy, γ = Wavelength; Inversely Proportional
(c) E = Energy, γ = Frequency; Inversely Proportional
(d) E = Energy, γ = Wavelength; Directly Proportional

Answer 8

(b) E = Energy, γ = Wavelength; Inversely Proportional

Question 9

A luminometer measures light emission via which of the following:

(a) Chemiluminescence
(b) Bioluminescence
(c) Electrochemiluminescence
(d) All of the above

Answer 9

(d) All of the above

Question 10

The measurement of light at selected wavelengths, typically in the ranges of ultraviolet (UV), visible, and infrared light, is known as:

(a) Photometry
(b) Refractometry
(c) Luminometry
(d) Spectrophotometry

Answer 10

(d) Spectrophotometry