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MLS 220 Clinical Lab Operations > Exam 4 > Flashcards

Exam 4 Flashcards

1
Q

Mass Spectrometry is a ________ technique.

A

Qualitative and Quantitative

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2
Q

Classify the following characteristics as those describing an Anode or a Cathode.

Positive Pole

A

Anode

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3
Q

Classify the following characteristics as those describing an Anode or a Cathode.

Negative Pole

A

Cathode

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4
Q

Classify the following characteristics as those describing an Anode or a Cathode.

Oxidation Reaction

A

Anode

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5
Q

Classify the following characteristics as those describing an Anode or a Cathode.

Reduction Reaction

A

Cathode

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6
Q

Classify the following characteristics as those describing an Anode or a Cathode.

Loss of Electrons

A

Anode

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7
Q

Classify the following characteristics as those describing an Anode or a Cathode.

Gain of Electrons

A

Cathode

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8
Q

Match the following instrumentation principles involving light energy with their descriptor.

A technique used to determine the concentration of a substance by measuring the variation in its color intensity without taking the wavelength of light into account

A

Photometry

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9
Q

Match the following instrumentation principles involving light energy with their descriptor.

The measurement of light at a selected wavelength, typically in the ranges of UV, visible, and infrared light

A

Spectrophotometry

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10
Q

Match the following instrumentation principles involving light energy with their descriptor.

The measurement of light reflected from the surface of a colorimetric reaction in order to determine the amount of unknown colored product

A

Reflectance Spectrophotometry

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11
Q

Match the following instrumentation principles involving light energy with their descriptor.

A type of spectrophotometry which uses a beam of light, typically UV light, to excite electrons in molecules causing them to emit light of a different wavelength

A

Fluorescent Spectrophotometry

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12
Q

Match the following instrumentation principles involving light energy with their descriptor.

An instrumentation technique based on detection of the portion of light scattered by the particles in a solution toward a detector not in the direct path of the transmitted light

A

Nephelometry

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13
Q

Match the following instrumentation principles involving light energy with their descriptor.

An instrumentation technique that measures the reduction of light transmitted through a homogeneous solution due to the light’s scattering

A

Turbidimetry

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14
Q

Match the following instrumentation principles involving light energy with their descriptor.

The measurement of light emission via chemiluminescence, bioluminescence, and electrochemiluminescence

A

Luminometry

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15
Q

A spectrophotometric ‘Blank’ is composed of:

A

All of the reagents used in the procedure without the sample

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16
Q

The color of light seen by the naked eye is _________ . All other wavelengths are _________.

A

Transmitted, Absorbed

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17
Q

Visible light constitutes approximately which wavelength:

A

380 – 700 nanometers

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18
Q

An Ion-Selective Electrode (ISE) is permeable to which of the following:

A

Membranes selectively permeable to certain ions ( H+,K+,Na+,Mg2+)

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19
Q

Mass Spectrometry separates molecules based on their:

A

Mass-to-Charge Ratio (m/z)

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20
Q

Select the correct relationship between frequency, wavelength, and energy.

A

↑ Frequency, ↓ Wavelength, ↑ Energy

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21
Q

Classify the following chromatographic separation mechanisms by the mechanism in which they separate analytes:

Separates analytes based on their charge using a charged stationary phase

A

Ion Exchange Chromatography

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22
Q

Classify the following chromatographic separation mechanisms by the mechanism in which they separate analytes:

Separates analytes based on the differential distribution of solutes between two immiscible liquids

A

Partition Chromatography

23
Q

Classify the following chromatographic separation mechanisms by the mechanism in which they separate analytes:

Separates analytes by adsorption and desorption of solutes at the surface of a solid particle

A

Adsorption Chromatography

24
Q

Classify the following chromatographic separation mechanisms by the mechanism in which they separate analytes:

Separates analytes via liquid chromatography using biological interaction (ie. enzyme binding a substrate, hormone with receptor, antibody with antigen, etc.)

A

Affinity Chromatography

25
Q

Classify the following chromatographic separation mechanisms by the mechanism in which they separate analytes:

Separate analytes based on their size

A

Size Exclusion Chromatography (gel permeation/gel filtration)

26
Q

A pH electrode measures the passage of which of the following ions:

A

H+ Ions

27
Q

The Clark Electrode is used to measure which of the following:

A

pO2

28
Q

The relationship between wavelength and energy is mathematically depicted using __________.

A

Plank’s Equation

29
Q

Chromatography can be classified based on two phases. Which of the following is NOT a phase used to classify chromatography:

A

Affinity Phase

30
Q

Osmolality is measured using which analytical technique:

A

Freezing Point Depression

31
Q

Proper procedure for using a pH meter requires a calibration curve to be constructed. This is necessary in order to calculate the ______ of the line, which is used to monitor drifting/electrode deterioration.

A

Slope

32
Q

Chromatography – particles in the mobile phase with a ______ affinity for the stationary phase migrate ______ than those with ________ affinity, which migrate ________.

A

higher / slower / lower / faster

33
Q

Chromatography is a _______ separation technique.

A

Physical

34
Q

Classifying a test as a Point of Care Test (POCT) refers to which of the following:

A

The physical location of where the test is to be performed

35
Q

Match the following Electrochemistry Technique to its correct descriptor.

The technique in which the electrical potential difference between two electrodes is measured under equilibrium

A

Potentiometry

36
Q

Match the following Electrochemistry Technique to its correct descriptor.

The technique that measures current flowing through an electrochemical cell and the electrochemical potential between the two electrodes while a constant external voltage is applied

A

Aperometry

37
Q

Match the following Electrochemistry Technique to its correct descriptor.

The technique that is used to measure the electrolytic conductivity of a half cell in order to monitor the progress of a chemical reaction

A

Conductometry

38
Q

Match the following Electrochemistry Technique to its correct descriptor.

The technique that measures the amount of charge, in coulombs, being passed between two electrodes at a fixed potential as a result of a chemical reaction

A

Coulometry

39
Q

The small vessel designed to hold a liquid sample in the light path of a spectrophotometer for measurement is known as a:

A

Cuvette

40
Q

In gas chromatography, ‘gas’ signifies the _____ phase:

A

Stationary

41
Q

The time required for a solute to pass through a chromatographic column and reach the detector is known as the:

A

Retention Time

42
Q

Electrochemistry involves the conversion of chemical energy into a flow of ______ within a galvanic cell.

A

Electrons

43
Q

Which of the following is true regarding specific gravity and freezing point depression:

A

Specific Gravity measures the presence of solutes represented by particles of varying sizes, from small ions to larger proteins. Freezing Point Depression does not rely on particle size.

44
Q

According to Beer’s law, a solution that absorbs a high amount of light would have a ______ concentration.

A

High

45
Q

Osmolarity expresses concentrations of solutions relative to the ____ of the solvent.

A

Volume

46
Q

The component of the spectrophotometer responsible for isolating radiant energy of a desired wavelength(s) while excluding other wavelengths is the:

A

Monochromator

47
Q

A solution with a pH of 7.6 is considered:

A

Basic

48
Q

The most common Indicator Electrode is known as the:

A

Ion Selective Electrode (ISE)

49
Q

Prior to separation/categorization using a mass spectrometer, molecules must first undergo:

A

Ionization

50
Q

Which of the following is an example of a planar form of chromatography:

A

Thin Layer Chromatography

51
Q

Osmolality expresses concentrations of solutions relative to the ____ of the solvent.

A

Mass

52
Q

The following chromatogram would be said to have _____.

A

Distinct peaks?

53
Q

Light is a form of electromagnetic radiant energy that travels in:

A

waves

MLS 220 Clinical Lab Operations (28 decks)

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