Exam 4 Flashcards
Mass Spectrometry is a ________ technique.
Qualitative and Quantitative
Classify the following characteristics as those describing an Anode or a Cathode.
Positive Pole
Anode
Classify the following characteristics as those describing an Anode or a Cathode.
Negative Pole
Cathode
Classify the following characteristics as those describing an Anode or a Cathode.
Oxidation Reaction
Anode
Classify the following characteristics as those describing an Anode or a Cathode.
Reduction Reaction
Cathode
Classify the following characteristics as those describing an Anode or a Cathode.
Loss of Electrons
Anode
Classify the following characteristics as those describing an Anode or a Cathode.
Gain of Electrons
Cathode
Match the following instrumentation principles involving light energy with their descriptor.
A technique used to determine the concentration of a substance by measuring the variation in its color intensity without taking the wavelength of light into account
Photometry
Match the following instrumentation principles involving light energy with their descriptor.
The measurement of light at a selected wavelength, typically in the ranges of UV, visible, and infrared light
Spectrophotometry
Match the following instrumentation principles involving light energy with their descriptor.
The measurement of light reflected from the surface of a colorimetric reaction in order to determine the amount of unknown colored product
Reflectance Spectrophotometry
Match the following instrumentation principles involving light energy with their descriptor.
A type of spectrophotometry which uses a beam of light, typically UV light, to excite electrons in molecules causing them to emit light of a different wavelength
Fluorescent Spectrophotometry
Match the following instrumentation principles involving light energy with their descriptor.
An instrumentation technique based on detection of the portion of light scattered by the particles in a solution toward a detector not in the direct path of the transmitted light
Nephelometry
Match the following instrumentation principles involving light energy with their descriptor.
An instrumentation technique that measures the reduction of light transmitted through a homogeneous solution due to the light’s scattering
Turbidimetry
Match the following instrumentation principles involving light energy with their descriptor.
The measurement of light emission via chemiluminescence, bioluminescence, and electrochemiluminescence
Luminometry
A spectrophotometric ‘Blank’ is composed of:
All of the reagents used in the procedure without the sample
The color of light seen by the naked eye is _________ . All other wavelengths are _________.
Transmitted, Absorbed
Visible light constitutes approximately which wavelength:
380 – 700 nanometers
An Ion-Selective Electrode (ISE) is permeable to which of the following:
Membranes selectively permeable to certain ions ( H+,K+,Na+,Mg2+)
Mass Spectrometry separates molecules based on their:
Mass-to-Charge Ratio (m/z)
Select the correct relationship between frequency, wavelength, and energy.
↑ Frequency, ↓ Wavelength, ↑ Energy
Classify the following chromatographic separation mechanisms by the mechanism in which they separate analytes:
Separates analytes based on their charge using a charged stationary phase
Ion Exchange Chromatography
Classify the following chromatographic separation mechanisms by the mechanism in which they separate analytes:
Separates analytes based on the differential distribution of solutes between two immiscible liquids
Partition Chromatography
Classify the following chromatographic separation mechanisms by the mechanism in which they separate analytes:
Separates analytes by adsorption and desorption of solutes at the surface of a solid particle
Adsorption Chromatography
Classify the following chromatographic separation mechanisms by the mechanism in which they separate analytes:
Separates analytes via liquid chromatography using biological interaction (ie. enzyme binding a substrate, hormone with receptor, antibody with antigen, etc.)
Affinity Chromatography
Classify the following chromatographic separation mechanisms by the mechanism in which they separate analytes:
Separate analytes based on their size
Size Exclusion Chromatography (gel permeation/gel filtration)
A pH electrode measures the passage of which of the following ions:
H+ Ions
The Clark Electrode is used to measure which of the following:
pO2
The relationship between wavelength and energy is mathematically depicted using __________.
Plank’s Equation
Chromatography can be classified based on two phases. Which of the following is NOT a phase used to classify chromatography:
Affinity Phase
Osmolality is measured using which analytical technique:
Freezing Point Depression
Proper procedure for using a pH meter requires a calibration curve to be constructed. This is necessary in order to calculate the ______ of the line, which is used to monitor drifting/electrode deterioration.
Slope
Chromatography – particles in the mobile phase with a ______ affinity for the stationary phase migrate ______ than those with ________ affinity, which migrate ________.
higher / slower / lower / faster
Chromatography is a _______ separation technique.
Physical
Classifying a test as a Point of Care Test (POCT) refers to which of the following:
The physical location of where the test is to be performed
Match the following Electrochemistry Technique to its correct descriptor.
The technique in which the electrical potential difference between two electrodes is measured under equilibrium
Potentiometry
Match the following Electrochemistry Technique to its correct descriptor.
The technique that measures current flowing through an electrochemical cell and the electrochemical potential between the two electrodes while a constant external voltage is applied
Aperometry
Match the following Electrochemistry Technique to its correct descriptor.
The technique that is used to measure the electrolytic conductivity of a half cell in order to monitor the progress of a chemical reaction
Conductometry
Match the following Electrochemistry Technique to its correct descriptor.
The technique that measures the amount of charge, in coulombs, being passed between two electrodes at a fixed potential as a result of a chemical reaction
Coulometry
The small vessel designed to hold a liquid sample in the light path of a spectrophotometer for measurement is known as a:
Cuvette
In gas chromatography, ‘gas’ signifies the _____ phase:
Stationary
The time required for a solute to pass through a chromatographic column and reach the detector is known as the:
Retention Time
Electrochemistry involves the conversion of chemical energy into a flow of ______ within a galvanic cell.
Electrons
Which of the following is true regarding specific gravity and freezing point depression:
Specific Gravity measures the presence of solutes represented by particles of varying sizes, from small ions to larger proteins. Freezing Point Depression does not rely on particle size.
According to Beer’s law, a solution that absorbs a high amount of light would have a ______ concentration.
High
Osmolarity expresses concentrations of solutions relative to the ____ of the solvent.
Volume
The component of the spectrophotometer responsible for isolating radiant energy of a desired wavelength(s) while excluding other wavelengths is the:
Monochromator
A solution with a pH of 7.6 is considered:
Basic
The most common Indicator Electrode is known as the:
Ion Selective Electrode (ISE)
Prior to separation/categorization using a mass spectrometer, molecules must first undergo:
Ionization
Which of the following is an example of a planar form of chromatography:
Thin Layer Chromatography
Osmolality expresses concentrations of solutions relative to the ____ of the solvent.
Mass
The following chromatogram would be said to have _____.
Distinct peaks?
Light is a form of electromagnetic radiant energy that travels in:
waves
