Lecture 12: Photosynthesis - 10/27 Flashcards
What are the 3 stages of the Calvin/C3 cycle?
Carboxylation
Reduction
Regeneration
What are the 3 important chemicals in the Calvin cycle?
3-PGA (3-phosphogylcerate)
G3P (glyceraldehyde-3-phosphate)
RuBP (Ribulose-1,5-biphosphate)
How does the Calvin cycle work?
RuBP, CO2, and H2O are used to produce 3-PGA, which is then reduced to form G3P.
For every 3 RuBP molecules, one G3P molecule is produced. Multiple G3P molecules are then synthesized into sucrose.
The rest of the G3P molecules are recycled back into RuBP.
Rubisco enzyme
Ribulose-1,5-biphosphate carboxylase/oxygenase
Catalyzes RuBP carboxylation (addition of CO2)
The most abundant protein on the planet. Make sure not to confuse this enzyme with RuBP, the substrate!
What is the net output, G3P, of the Calvin cycle used for?
Building starch in chloroplasts
Making sucrose in the cytosol
Entering glycolysis in the cytosol to contribute to cellular respiration
Transitory starch
Starch that is made during the day and stored in the stroma. It is degraded at night so the plant has energy
Storage starch
Can be made at any time and are deposited in sink tissues. The synthesis of this kind of starch occurs in amyloplasts
How does the intensity of light effect photosynthesis?
The more intense the light, the greater the rate of light reactions
Shade leaves
Thinner leaves with greater area
Sun leaves
Thicker and less wide
Why is there carboxylase/oxygenase in Rubisco’s name?
Because it can act as both a carboxylase and oxygenase
If CO2 is used, the reaction in the Calvin cycle will produce two 3-phosphoglycerate (has 6 carbons)
If O2 is used, the reaction will produce 3PGA and 2-phosphoglycolate (5 carbons). This is because there wasn’t a C from CO2