Lecture 11 Reading Flashcards
Neurocircuitry of stress: central control of the hypothalamo-pituitary-adrenocortical axis
What are systemic stressors and how are they relayed to the PVN?
What are processive stressors and how are they relayed to the PVN?
What do functional and neuroanatomical data suggest are involved in inappropriate stress control?
Systematic stressors that involve an immediate physiological threat are relayed directly to the PVN of the hypothalamus via brainstem catecholaminergic projections ○ (catecholamines include dopamine, epinephrine, norepinephrine)
Processive stressors that require interpretation by higher brain structures are channeled through limbic forebrain circuits ○ These limbic sites connect with the PVN via interactions with GABA neurons in the bed nucleus of the stria terminalis
Functional and neuroanatomical data suggest that disease processes involving inappropriate stress control involve dysfunction of processive stress pathways
Central coordination of glucocorticoid release: role of the PVN
If there is a lesion of the PVN what happpens?
What happens to CRH vesicles if there is stimulation of the PVN?
PVN neurons release ACTH secretagogues (substances that promote secretion) of CRH and arginine-vasopressin (AVP)
Lesion to PVN → reduction in ACTH and CRH levels
Stimulation of the HPA system is marked by depletion of CRH- and AVP-containing neurosecretory vesicles in the median eminence, which is indicative of ACTH release
Initiating the stress response
What does the amydala do?
What does the bed nucleus of stria terminalis (BST) do?
Catecholaminergic drive appears to promote HPA secretory activity following hemorrhage, hypotension and respiratory distress, and might play a role in ACTH responses to immune challenge
HPA-excitatory information may be communicated via the amygdala
- Amygdala is known to prompt behavioral and cardiovascular responses to stress
- Stimulation of medial or cortical amygdaloid nuclei elicits corticosterone secretion
Bed nucleus of stria terminalis (BST) may also convey excitation to HPA axis
BST links regions such as the amygdala and hippocampus with hypothalamic and brainstem regions
- Ablation to this area decreases mRNA production of CRH whereas stimulation increases corticosterone release
- Activation of stress axis may be affected by ascending aminergic input from the locus coeruleus of the pons (noradrenaline) and raphé nuclei of the brainstem (5-HT, serotonin)
- But the role of these regions is up for debate
Glutamate-containing neurons and maybe acetylcholine-containing neurons also play a role in excitation of the PVN
Limiting the stress response
What are the negative feedback routes
Glucocorticoids negative feedback acts at the PVN itself
Neuronal inhibitory pathways work in parallel with steroid feedback
Hippocampus displays the highest levels of glucocorticoid binding
- Lesions potentiate stress-induced glucocorticoid secretion in rats and primates, and increase the expression of CRH and AVP mRNA in parvocellular PVN neurons
- Stimulation decreases HPA activity
PFC and lateral septum damage results in enhanced HPA responsiveness to acute stress
PVN neurons receive direct inhibitory input from local hypothalamic circuits
GABA is known to inhibit the release of ACTH and corticosterone in vivo
What are limbic stressors and what do they respond to?
What do they require?
What does excitatory outflow from the hippocampus achieve in terms of HPA activity?
How do BST, preoptic, and hypothalamic cell groups work together?
Limbic stress pathways are most sensitive to stressors involving higher order sensory processing
-Ex: HPA responses to restrain, fear conditioning, or exposure to a novel environment are affected by PFC, hippocampus, or amygdala lesions
Limbic-sensitive stressors are processive
- They require a sequential stimulus assembly to obtain physiologic meaning
- Key characteristic of processive stress integration: need for an intervening synapse between limbic sites and PVN
Interaction of hippocampal efferents with GABA-containing neurons from BST, preoptic area, or hypothalamus suggests a capacity for excitatory outflow from the hippocampus to achieve a net inhibitory effect on the PVN
BST, preoptic, and hypothalamic cell groups integrate limbic input with homeostatic info prior to final elaboration of the stress response
Brain stress regulation in disease
GABA modulates stress-induced glucocorticoid secretion in rats and humans and has been identified as a mitigating factor in major depressive disorder
Systemic stressors
Are these limbic sensitve or insensitive?
HPA responses to physiological threats (e.g. hypoxia, lack of oxygen) are not affected by lesions to the limbic system
-In these situations, rapid relay of an excitatory signal to the PVN occurs via the brainstem, bypassing the need for cognitive processing
Limbic-insensitive stressors are systemic
-These stressors are of immediate survival value and do not require interpretation by higher order brain structures
Do all processive and systemic stressors do use the same mechanisms?
All stressors within the two classes do not use identical circuitry i.e. Specific processive stressors should elicit characteristic patterns of limbic activation
NO!
The Sympathetic Nervous System
The sympathetic nervous system (SNS) is the most important system associated with the stress response
-Fight or flight
Adrenaline released from adrenal medulla and noradrenaline from sympathetic nerves results in an increase in heart rate, peripheral vasoconstriction, and energy mobilization
PVN helps integrate stress signals
This system is activated in response to physical threats and in reaction to bodily events (e.g. injury, infection)
System returns to normal once the source of stress is terminated
HPA Axis basics
Outline of hormones
Timeline from start to finish
HPA axis becomes activated when threats and negative consequences are anticipated, even well before they have occurred
System can be active over longer periods of time, even if the event never occurs (e.g. an important deadline, exam)
More reactive to psychosocial stressors
CRH from hypothalamus → ACTH from pituitary → glucocorticoids from adrenal cortex (cortisol in humans, corticosterone in rats)
Time between stress onset and peak in secretion of cortisol is 15-30 mins
Cortisol levels return to baseline about on hour after a stressor stops
Subjective emotional experience of stress in the CNS
What did Lazarus argue?
How might individual differences influence this?
What brain regions play a part?
Cognitive factors determine the initial appraisal of a situation as stressful
Lazarus argued that an individual’s appraisal of a situation as threatening, which is determined by a comparison of the significance and demand of the situation with the available resources, will cause the individual to perceive a situation as stressful and initiate the physiological and endocrinological responses to stress
Psychological factors (e.g. self-esteem, coping style) and activated memories of past threats play a role in the perception of stress
Regions that have been identified to be involved in this process -Anterior cingulate gyrus
- Ventromedial PFC
- Orbitofrontal cortex
- Amygdala (and the medial temporal lobes in general)
Assessment of the subjective emotional stress experience (how do we do it?)
How does this combine with other psychometrics?
Includes measures of perceived stress and cognitive appraisal ○ Primary appraisal secondary appraisal scale
- COPE inventory
- Profile of mood states
Can be combined with measures of key personality traits such as -Self-esteem
- Extraversion
- Neuroticism
Assessment of the SNS (how do we do it?)
6 things
Can look at catecholamine release, heart rate, blood pressure, galvanic skin response
Cardiac imaging
Measure salivary alpha-amylase
Measurement of HPA axis activity
What is the most common measure?
Is there a weakness with this?
ACTH and cortisol measured from blood plasma
Most common technique: salivary cortisol
- Easy to obtain samples from participants
- But it is really only a fraction of the final product of the activation of the HPA axis, perhaps not getting the whole picture by using this measure
THE INTERPLAY BETWEEN THE SYSTEMS INVOLVED IN STRESS PROCESSING
Why is there incongruence?
“Response coherence”: the idea that the initial stressor causes a coordinated response at the level of the subjective emotional experience, and the behavioral, physiological, and endocrine systems
Would expect that being exposed to a stressful situation leads to a perception of that situation, and the activation of both the SNS and HPA THIS DOES NOT HAPPEN!
Several factors could play a role in this incongruence -Individual’s appraisal of their own emotional reaction
-Individual might possess specific personality characteristics that would make it more difficult to accurately be aware of emotions resulting in distorted self-perception and reporting ○ Interaction between systems might be different from coherence hypothesis