Lecture 10 Blood (Connective Tissue IV)

Question 1

Percent of WBC

Answer 1

neutrophils 60

eosinophils 1-3

basophils>1

lymphocytes 25-30

monocytes3-7

Question 2

______ stains typically used on blood smears

mixture of acidic (eosin) and basic (methylene blue) (stain nucleus) dyes and azures that stain cytoplasmic granules (lysosomes) of leukocytes by ______.

Answer 2

G wright, metachromasia

Question 3

true or false: RBC is basophilic

Answer 3

False, acidophilic/eosinophillic

Question 4

What IS the shape of the nucleus for of the leukocytes.

Answer 4

Neutro, multilob

eosin, bilob

baso, bilob

mono, c shape

lympho, sphericAL

Question 5

WHat are the granulocytes, what are there granule colours?

Answer 5

Neutro (pale pink), eosin (red), baso (blue)

Question 6

What leukocyte is this? What are its properties?

Answer 6

12-15 microm, 2x sixe of RBC

• Multilobed nucleus
• Pale pink cytoplasmic granules
• Killa bactera
• NOT ACTIVE IN BLOOD –> infiltrate tissue (Apoptosis)
• Short lived (hours), macrophages clean them up

Question 7

What leukocyte is this? What are its properties?

Answer 7

eosinophile

Diameter – 12-25microm

• Bilobed nucleus
• Eosinophilic granules – contain major basic protein, crystalline structure
• Kill parasitic worms + helminths

inflammatory response

Question 8

What leukocyte is this? What are its properties?

Answer 8

basophile

• Diameter = 12-15 microm
• < 1%
• Bilobed nucleus, “S shape” obsecured by basophilic stainiting
• Inflammation: supplementing mast cells
• smaller

Question 9

What leukocyte is this? What are its properties?

Answer 9

Lymphocyte

• Smallest white blood cell but vary in size, same size as RBC
• Spherical nucleus
• Cytoplasm limited to the periphery of the cell
• Adaptive immunity: T + B lymphocytes, natural killer cell
• need a marker to differentiate between specialized cell

Question 10

What leukocyte is this? What are its properties?

Answer 10

Monocyte

• 12 – 20 microm
• Mononuclear phagocytic system
• Indented or “C” shape nucleus
• Slightly basophilic cytoplasm
• Phagocytosis: garbage collector
• Antigen presentation

Question 11

______ are precursors to macrophages, osteoclasts, microglial cells

Answer 11

monocytes

Question 12

What is this structure? Whats its characteristics

Answer 12

Platelet

Thrombocytes: non-nucleated cell fragments

• 2-4 mircom, small
• Separated in zones , outside = hylomere, inside = granulomere
• Involved in clot formation

OCS open canicular system

• Found in hylomere
• Increase in surface area
• Uptake and release of content in granules

Question 13

What are the components of a Giema-Wright stain?

Answer 13

Azure stain – metachromatic methylene blue eosin