LEC: Sterilization and Disinfection

Question 1

it is the complete removal or destruction of all forms of microorganisms including endospores

Answer 1

sterilization

Question 2

destruction of microbes that can cause disease; can kill some type of microbes but not all.

Answer 2

disinfection

Question 3

can kill some spores but not all that is present

Answer 3

disinfection

Question 4

differentiate sterilization and disinfection

Answer 4

sterilization is the complete removal of all forms of microorganisms including the spores; disinfection, on the other hand, is the destruction of some microbes that can cause disease, and may also kill some spores but it can’t kill all microbes that is present.
(Key terms: sterilization –> complete removal including spores; disinfection –> destruction of some microbes but can’t kill microbes and spores)

Question 5

it is the destruction or inhibition of microorganisms that are present in the skin or a living tissue

Answer 5

antisepsis

Question 6

it is the chemical agent used in limiting or preventing the number of bacterial growth in a living tissue

Answer 6

antiseptic

Question 7

define antisepsis

Answer 7

it is the destruction of inhibition of microorganisms present in the a living tissue.

Question 8

TRUE OR FALSE: both sterilization and antisepsis are sporicidal

Answer 8

FALSE; only sterilization or capable of killing endospores.

Question 9

these two methods are critical in the day to day tasks inside the microbiology laboratory

Answer 9

sterilization and disinfection

Question 10

this technique is used in the following:
- microbiological research
- preservation of food
- prevention of disease

Answer 10

aseptic technique

Question 11

aseptic technique is typically used in (give 3 examples)

Answer 11

• microbiological research
• preservation of food
• prevention of disease

Question 12

these are the requirements of a laboratory that works with pure cultures

Answer 12

sterile apparatus and culture media