LAB: Bacterial Smear Preparation Flashcards
these are used to apply color to bacteria for it to be seen and observed under microscope
staining reagents
it is done to make sure that the bacteria will remain attached to the glass slide
heat fix
basic stain is applied in what type of staining
Simple staining
how does staining takes place in a bacteria
considering the fact that stain are positively charged, the negatively charged surface and cytoplasm of a bacteria will attract to it, thus staining happens
key terms:
- positively charged stains
- negatively charged surface of bacteria
- cytoplasm of bacteria
a type of staining technique where only one stain is used
simple stain technique
a type of culture where only one species bacterium is present
pure culture
define pure culture
a laboratory culture that contains only a single species of organisms
what is the difference of pure culture and a colony
a pure culture means that there’s only one strain of organism present; a colony on the other hand, group of organisms living in close associations, and is a product of exponential bacterial cell division
TRUE OR FALSE: a colony is considered as a pure culture
TRUE; because it is basically just a group of organism belonging in a same species
bacteria can be cultivated in a (2 types of media)
liquid growth medium and solid growth medium
it is a liquid growth medium
nutrient broth
a solid growth medium
nutrient agar
it is thin layer of bacteria placed in a slide for staining
bacterial smear
TRUE OR FALSE: bacterial smear is a thick layer bacteria placed in a slide
FALSE; in bacterial smear, the bacteria placed in a slide should be of a thin layer
TRUE OR FALSE: it is okay to drag the 40x objective lens through the immersion oil
FALSE: allowing the oil to touch the other objective lenses (aside from the OIO) will damage their lens, rendering them useless
it is used to wipe off the oil from the OIO lens
lens paper
heat fix is passing the slide through the flame by how many times
3-4 times
sources of flame in the laboratory
bunsen burner and denatured alcohol
the suggested size of bacterial smear
five-centavo coin
TRUE OR FALSE: bacterial smear should be heat-fixed first before air-drying
FALSE; after allowing the liquid to evaporate during the air-drying, bacterial smear should then be heat-fixed.
the main goal this technique is to maintain the sterile environment and avoid contamination of unwanted microorganism
aseptic technique
