Lec 4 Flashcards

1
Q

What type of microscopy is this?

A

Brightfield

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2
Q

What type of microscopy is this?

A

Dark Field

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3
Q

What type of microscopy is this?

A

Phase Contrast

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4
Q

What type of microscopy is this?

A

Fluorescent Microscopy

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5
Q

What type of microscopy is this?

A

Confocal Microscopy

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6
Q

What type of microscopy is this?

A

Electron – Scanning

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7
Q

What type of microscopy is this?

A

Electron – transmission

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8
Q

What staining kills bacteria

A

Heat Fixation

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9
Q

Postively charged stains are also known as

A

Simple (morphological) = basic stains

methylene blue, crystal violet

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10
Q

Acidic stains are also known as =
The specimen is what color?

A

Negative stains – no heat fixation used – specimen =m stark white

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11
Q

What bacterical structure takes up gram staining and shows up purple?

A

Gram Positive

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12
Q

Identify gram stain and shape

A

Gram +
Bacillus

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13
Q

Identify gram stain and shape

A

Gram + Coccus
and
Gram - Coccus

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14
Q

Identify gram stain and shape

A

Gram -
Bacillus

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15
Q

Identify gram stain and shape

A

Gram -
Coccus

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16
Q

What differential staining is this?

A

Capsule Stain
composed of polysacharrides

17
Q

What type of differential stain is this?

A

Acid-Fast Stain – detects the presence of mycolic acid in mycobacterium

18
Q

Name the Flagellar Staining

A

Monotrichous – single flagellum (polar)

19
Q

Name the Flagellar Staining

A

Amphitrichous

20
Q

Name the Flagellar Staining

A

Lophotrichous

21
Q

Name the Flagellar Staining

A

Peritrichous

22
Q

Name the differenital staining:

A

Endospore Stain

23
Q

endospores contain what which allows it to produce a impenetrable barrier

A

Calcium Dipicolinate

24
Q

What happens to endospore cytoplasm to prevent the conductance of heat

A

Dehydration