Lec 4 Flashcards
(24 cards)
What type of microscopy is this?
Brightfield
What type of microscopy is this?
Dark Field
What type of microscopy is this?
Phase Contrast
What type of microscopy is this?
Fluorescent Microscopy
What type of microscopy is this?
Confocal Microscopy
What type of microscopy is this?
Electron – Scanning
What type of microscopy is this?
Electron – transmission
What staining kills bacteria
Heat Fixation
Postively charged stains are also known as
Simple (morphological) = basic stains
methylene blue, crystal violet
Acidic stains are also known as =
The specimen is what color?
Negative stains – no heat fixation used – specimen =m stark white
What bacterical structure takes up gram staining and shows up purple?
Gram Positive
Identify gram stain and shape
Gram +
Bacillus
Identify gram stain and shape
Gram + Coccus
and
Gram - Coccus
Identify gram stain and shape
Gram -
Bacillus
Identify gram stain and shape
Gram -
Coccus
What differential staining is this?
Capsule Stain
composed of polysacharrides
What type of differential stain is this?
Acid-Fast Stain – detects the presence of mycolic acid in mycobacterium
Name the Flagellar Staining
Monotrichous – single flagellum (polar)
Name the Flagellar Staining
Amphitrichous
Name the Flagellar Staining
Lophotrichous
Name the Flagellar Staining
Peritrichous
Name the differenital staining:
Endospore Stain
endospores contain what which allows it to produce a impenetrable barrier
Calcium Dipicolinate
What happens to endospore cytoplasm to prevent the conductance of heat
Dehydration