Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

CC1 > Lec 3 > Flashcards

Lec 3 Flashcards

1
Q

measures light and is applied to spectrophotometry

A

Colorimetry

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Law of colorimetry

A

Involves measurement of the amount of light absorbed by the solution, and that absorption is related to concentration.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

2 primary considerations in colorimetric analysis

A

• quality of light
• intensity of light

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

A kind of colorimetry that uses the eye in determining the end product/point.

A

Visual Colorimetry

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Duboscq Colorimeter follows _____ Law.

A

Beer’s Law

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

A = C x L x K

A

Beer’s Law formula

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

The solution’s absorbance is directly proportional to the solution’s concentration

A

Beer’s Law

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

The solution’s absorbance is inversely proportional to the transmittance

A

Beer’s Law

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

A refined type of visual colorimeter. It is used to determine the concentration of UNKNOWN GLUCOSE solutions

A

Duboscq Colorimeter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

The concentration of the absorbance is equal to the concentration of the substance times the depth

A

Beer’s Law

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Spectrophotometry

A

Measures light intensity using a device (prism/grating) to disperse the source of light into a continuous spectrum.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Measures light intensity of multiple wavelength using a filter to isolate part of the spectrum

A

Filter Photometry

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Entrance slit is also known as _____

A

Collimator lens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

It narrows down the rays of light coming from the light source

A

Entrance slit

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

This separates the white light into different colors, and only one of those colors is allowed to pass through.

A

Monochromator

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

It provides radiant energy in the form of visible light that passes through the monochromator.

A

Light Source

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

8 types of light source

A
  • Tungsten lamp
  • hydrogen lamp
  • quartz halide lamp
  • deuterium lamp
  • Mercury vapor/arc lamp
  • Hollow cathode lamp
  • LED (light emitting diodes)
  • Laser
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

This light source provided energy wavelength from 340 to 780 nm and wavelength is visible.

A

Tungsten Lamp

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Tungsten lamp that is for visible and UV

A

Tungsten Iodine Lamp

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Tungsten Lamp which is the filament of the lamp.

A

Tungsten Halide Filaments

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

TRUE or FALSE:

Tungsten Halide Filaments
- long lasting
- less light at shorter wavelength
- emits a higher intensity radiant energy

A

FALSE

  • MORE light at shorter wavelength
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

A light source with a wavelength less than 300 nm

A

Deuterium Lamp

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

This part of the spectrophotometer minimizes stray light from the light source by preventing scattering of light.

A

Entrance Slit

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Monochromator

A

A wavelength selector which isolate specific wavelength of light by the use of a prism/grating or both.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

4 types of monochromator

A

Prism, Diffraction grating, Colored filter, Interference filter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

A type of monochromator that is a triangular wedge-shaped piece. Made of either glass, quartz, NaCl or KBr.

A

Prism Monochromator

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

A prism monochromator for visible spectrum

A

Glass prism

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

A prism monochromator used in UV

A

Quartz / Fused silica prism

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

A prism monochromator for infrared spectrum

A

Prism made of NaCl or KBr

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

A monochromator that has small grooves cut into it. May have >3000 grooves per millimeter

A

Diffraction Grating

31
Q

Colored Filter

A

A type of monochromator that allows a wide range of wavelengths to pass through wide bandwidths of 20-60nm. Less expensive but less sensitive

32
Q

A type of monochromator that is used to obtain special purity.

A

Interference Filter

33
Q

General methods under the classifications of Clinical Chem instrumentation that measure EMR

A

Spectrophotometry,
Electrophoresis,
Immunoassay,
Nephelometry,
Ion-selective electrodes

34
Q

Basic Categories of Analytical Chem

A

Spectrometry,
Luminescence,
Electroanalyctical methods,
Chromatography

35
Q

The measurement of radiant energy that is absorbed or given off by a molecule after illumination of light source.

A

Photometry

36
Q

The radiant energy that includes short gamma rays to long radio waves. They are photons of energy traveling in a wave-like manner.

A

EMR

37
Q

Types of EMR (in order)

A

Cosmic rays,
Gamma rays,
X-rays,
UV rays,
Visible rays,
Infrared,
Radio waves

38
Q

It is a photon or discrete packet of energy traveling in waves.

A

Light

39
Q

Wavelength of light

A

The distance between 2 successive peaks.

40
Q

Value of h (Planck’s constant)

A

6.62 x 10^(-27)

41
Q

Refers to the wavelength interval in which the lowest type of radiant energy occurs

A

Planck’s constant

42
Q

A term used to define a light wave. It is the distance between trough & peak

A

Amplitude

43
Q

Nanometer (nm)

A

• 10^(-9)
• the standard international unit for expressing wavelength of light

44
Q

• old term for wavelength
• 1 mu = 1 nm

A

Millimicron (mu)

45
Q

Angstrom (A)

A

• smaller compared to Nanometer and Millimicron.
• 10^(-10)

46
Q

A kind of wavelength with approximately 390 to 750 nm

A

Visible spectrum/spectra/light/ray

47
Q

2 rays under Invisible Spectra

A

UV rays, Infrared ray

48
Q

A kind of invisible spectrum with a wavelength of 190 to 390 nm

A

UV rays

49
Q

A kind if invisible spectrum with a wavelength of more than 800 nm

A

Infrared rays

50
Q

Delivers the light desired wavelength by adjusting the wavelength adjustment knob

A

Exit slit

51
Q

A certain range of band of wavelengths are allowed to pass into the sample

A

Bandpass/Bandwidth

52
Q

An absorption cell used to hold a solution inside the instrument. Absorption is measured.

A

Analytical Cell / Cuvette / Sample Holder

53
Q

A kind of cuvette used for solutions that are alkaline and do not etch the glass.

A

Soft glass cuvette

54
Q

A kind of cuvette suitable for visible portion of light

A

Borosilicate glass cuvette

55
Q

A kind of cuvette that does NOT absorb UV radiation, so it is used to measure UV radiation (below 320 nm)

A

Quartz / Plastic cuvette

56
Q

An electron tube amplifying current that can convert transmitted radiant energy into an equal amount of Photoelectric energy

A

Detector

57
Q

A type of detector that is less expensive, durable, and temperature sensitive.

A

Barrier-layer cell / Photocell

58
Q

A type of detector that gives off electrons when light energy strikes. It requires an outside voltage, thus it’s expensive.

A

Photoemission Tube

59
Q

A type of detector that detects and amplifies radiant energy from the solution to the detector, wherein it is already converted to electrical energy.

A

Photomultiplier

60
Q

A type of detector which produces a photocurrent that is proportional to the incident radiant energy.

A

Photodiode

61
Q

The simplest method of displaying the output of the detection system.

A

Data read-out device

62
Q

It is a reference solution when electrical read out of the instrument is arbitrarily at 100%T or 0.0 A

A

Blanking / Blank (BLK)

63
Q

4 types of BLK

A

Distilled water blank,
Reagent blank,
Air blank,
Serum / Sample blank

64
Q

A type of BLK which contains only distilled H2O. It is used to set Zero Absorbance (0.0 A)

A

Distilled water blank

65
Q

A type of BLK that contains all the components of the chromogenic reaction EXCEPT the sample and its analyte.

A

Reagent Blank

66
Q

A type of BLK that contains all the reagent and the sample. EXCEPT for the last reagent that will give color to the reaction.

A

Sample BLK

67
Q

A measure of the amount of light that passes through the solution

A

Transmittance

68
Q

A proportion of light that penetrates the solution

A

Transmittance

69
Q

The amount of light being absorbed or stopped by a solution

A

Absorbance

70
Q

Formula used in the Spectrophotometer

A

Cu = Au x Cs / As

71
Q

5 Calibration procedures for Quality Assurance of Spectrophotometry.

A

Wavelength calibration,
Absorbance/Photometric Accuracy checks,
Linearity check,
Electronic Stability,
Stray light check

72
Q

The 3 standards for Photometric Accuracy Checks

A

Potassium dichromate sol,
Cobalt ammonium sulfate sol,
Potassium nitrate sol

73
Q

Any wavelength of light outside the band transmitted and selected by the monochromator

A

Stray light

CC1 (16 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions