Labratory Diagnosis Ch9 Flashcards

1
Q

Thin flexible thin wall

A

Spirosheets

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2
Q

Serologic test

A

In the serum of pateient (organisim specific antibody) + antigen
For identifaction and diagnosos

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3
Q

STAT test

A

Immediate test

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4
Q

Bacteriological approach

A

Bacteria diagnosos (stain + growth)

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5
Q

Immunolgical approach (sereologic)

A

Diagnose the antibody of pathogen

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6
Q

Susceptibility test

A

Put antibiotics وبنفحص اذا في inhibition zone

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7
Q

IgM antibody

A

The primary responce وبحدد اذا في Current infection

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8
Q

IgG

A

Unclear response اذا ال current infictionاو previous infection

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9
Q

The types of blood agar media

A

1- alpha-hemolytic: oxidase (fe) ,green
2- beta-hemolytic: compltely ruptureللبكتيريا , halo (فراغ حول البكتيريا)
3-gama-hemolytic: non hemolyriv نادرا ما يكون في خطوط

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10
Q

Typticsoy agar

A

Contain : casien soybean
Growth for fastidious and non fastidous bacteria
بحوّل الorganic nitrogen into amino acids \polypeptides اللي بيجعل الوسط غني بالغذاء

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11
Q

Spirosheets

A

Flexible thin wall

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12
Q

Bacteria not grow in blood agar (intracellular bacteria)

A
  • mycoplasma
  • chlamydia
  • rickettisia
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13
Q

Bبكتيريا لا تنمو في ال blood agar الا بدرجات حرارة عالية

Choclate agar+cooked agar

A

Haemophillus

Nesseria

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14
Q

Bacteria that growth in macConkey agar

A

Gram(-) bacteria

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15
Q

The type of infection to use blood culture

A

1- carditis
2-meninges
3-pneumonia
4-ostemylitis

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16
Q

Bacteria in blood culture

A
3 gram(-) bacteria
1-psedomonus aerginosa
2-klibilli pneumonia
3-ischerchia coli
2 gram+
1-streptococcus pneumonia
2-staphylococcus ureus
17
Q

Bacteria found in CSF culture

A

1- s.pneumonia
2-nesseria meningetis
3-haemophilus influenza

18
Q

Acid fast bacteria in meninges

A

1-mycobacterium tubreculosis

2-fungus cryptococcus neoformans

19
Q

Bacteria in immunological test

A
1-s.pneumonia
2-h.influenzae
3-s.neoformans
4-e.coli
5-n.meninges
6-group B streptococci
20
Q

Bacteria found in urine culture

A

1- enterobactar
2-enterococcus faiclis
3-e.coli
4-proteus

21
Q

Ways to take a specimen from urine culture

A

1-catheterization

2-suprabubic aspiration

22
Q

Bacteria and how cultured in genital culture?

A

Nessiseria gonorrhoeae

Detect in gram stain in microscobic examination

23
Q

Bacteria cause cevicitis and urethritis?

A

Chlamidia tracomates detect in sereologic test

24
Q

How detect the syphilus

A

Serologic or microscopic dark field by appear motile spirosheets

25
Q

Bacteria cause surgical wound infection

A
Anaerobic bacteria (bacteroid fragilis)
Positive bacteria (s.aureus\s.biogenes)
Flora(clostridium perfrengens)
26
Q

Antiserum

A

Serum بكون في antibody against antigen في الجسم للعلاج من البكتيريا
في العادة بكون against capsular bacteria

27
Q

Bacteria اللي بتعمل reaction with antiserum

A

1- pneumonia
2-h.influnzae type b
3 n.meninges group a+c

28
Q

How detect shlagella and salmonella in slide agglutination test?

A
  • Shlagella and salmoneela -put antibody agains O cell wall antigen
  • Salmonella - put antibody against flagella H and capsular Vi antigens
29
Q

Use blood culture when:

A
1-sepsis
2-endocarditis
3-meningitis 
4-pneumonia
5-osteomyelitis
30
Q

What is a direct examination in stool sulture

A

Mythelne blue stain that stain leukocyte to detect exogenous bacteria

31
Q

What cause the antiserum

A

Capsular swelling (quellung reaction)

32
Q

Bacteria identified by slide agglutination test

A

1-shigella

2-salmonella

33
Q

Bacteria that detect in counter-immunoelectrophoresis test

A

That presen e in spinal fluid (detect capsular antigen)

-group B streptococci

34
Q

Enzyme-linked assay(ELISA)

A

To detect the homologous antigen

By bounded substrate into second antibody(eazyme)lead to color change

35
Q

Non treponemal test for syphilus detection

A

Cardiolpin-licithinncholestrol as antigen

VDR\RPR testes

36
Q

Use throat culture when:

A
  • pharyngitis
  • diaphthrtia
  • thrush
37
Q

Ways to growth bacteria in sputum culture:

A
  • blood agar
  • serilogical test
  • acid fast (mycobacterium)
  • antibody titer (mycoplasma)
  • anaerobic culture
38
Q

How we detect acute meningitis bacteria

A

By seriological test (immonolgical test) because they capsulated do quellung reaction

38
Q

Ways to growth bacteria in CSF

A
  • serological test
  • gram stain
  • acid fast stain (need 6 weeks)