Labratory Diagnosis Ch9

Question 1

Thin flexible thin wall

Answer 1

Spirosheets

Question 2

Serologic test

Answer 2

In the serum of pateient (organisim specific antibody) + antigen
For identifaction and diagnosos

Question 3

STAT test

Answer 3

Immediate test

Question 4

Bacteriological approach

Answer 4

Bacteria diagnosos (stain + growth)

Question 5

Immunolgical approach (sereologic)

Answer 5

Diagnose the antibody of pathogen

Question 6

Susceptibility test

Answer 6

Put antibiotics وبنفحص اذا في inhibition zone

Question 7

IgM antibody

Answer 7

The primary responce وبحدد اذا في Current infection

Question 8

IgG

Answer 8

Unclear response اذا ال current infictionاو previous infection

Question 9

The types of blood agar media

Answer 9

1- alpha-hemolytic: oxidase (fe) ,green
2- beta-hemolytic: compltely ruptureللبكتيريا , halo (فراغ حول البكتيريا)
3-gama-hemolytic: non hemolyriv نادرا ما يكون في خطوط

Question 10

Typticsoy agar

Answer 10

Contain : casien soybean
Growth for fastidious and non fastidous bacteria
بحوّل الorganic nitrogen into amino acids \polypeptides اللي بيجعل الوسط غني بالغذاء

Question 11

Spirosheets

Answer 11

Flexible thin wall

Question 12

Bacteria not grow in blood agar (intracellular bacteria)

Answer 12

• mycoplasma
• chlamydia
• rickettisia

Question 13

Bبكتيريا لا تنمو في ال blood agar الا بدرجات حرارة عالية

Choclate agar+cooked agar

Answer 13

Haemophillus

Nesseria

Question 14

Bacteria that growth in macConkey agar

Answer 14

Gram(-) bacteria

Question 15

The type of infection to use blood culture

Answer 15

1- carditis
2-meninges
3-pneumonia
4-ostemylitis

Question 16

Bacteria in blood culture

Answer 16

3 gram(-) bacteria
1-psedomonus aerginosa
2-klibilli pneumonia
3-ischerchia coli
2 gram+
1-streptococcus pneumonia
2-staphylococcus ureus

Question 17

Bacteria found in CSF culture

Answer 17

1- s.pneumonia
2-nesseria meningetis
3-haemophilus influenza

Question 18

Acid fast bacteria in meninges

Answer 18

1-mycobacterium tubreculosis

2-fungus cryptococcus neoformans

Question 19

Bacteria in immunological test

Answer 19

1-s.pneumonia
2-h.influenzae
3-s.neoformans
4-e.coli
5-n.meninges
6-group B streptococci

Question 20

Bacteria found in urine culture

Answer 20

1- enterobactar
2-enterococcus faiclis
3-e.coli
4-proteus

Question 21

Ways to take a specimen from urine culture

Answer 21

1-catheterization

2-suprabubic aspiration

Question 22

Bacteria and how cultured in genital culture?

Answer 22

Nessiseria gonorrhoeae

Detect in gram stain in microscobic examination

Question 23

Bacteria cause cevicitis and urethritis?

Answer 23

Chlamidia tracomates detect in sereologic test

Question 24

How detect the syphilus

Answer 24

Serologic or microscopic dark field by appear motile spirosheets

Question 25

Bacteria cause surgical wound infection

Answer 25

``` Anaerobic bacteria (bacteroid fragilis) Positive bacteria (s.aureus\s.biogenes) Flora(clostridium perfrengens) ```

Question 26

Antiserum

Answer 26

Serum بكون في antibody against antigen في الجسم للعلاج من البكتيريا في العادة بكون against capsular bacteria

Question 27

Bacteria اللي بتعمل reaction with antiserum

Answer 27

1- pneumonia 2-h.influnzae type b 3 n.meninges group a+c

Question 28

How detect shlagella and salmonella in slide agglutination test?

Answer 28

- Shlagella and salmoneela -put antibody agains O cell wall antigen - Salmonella - put antibody against flagella H and capsular Vi antigens

Question 29

Use blood culture when:

Answer 29

``` 1-sepsis 2-endocarditis 3-meningitis 4-pneumonia 5-osteomyelitis ```

Question 30

What is a direct examination in stool sulture

Answer 30

Mythelne blue stain that stain leukocyte to detect exogenous bacteria

Question 31

What cause the antiserum

Answer 31

Capsular swelling (quellung reaction)

Question 32

Bacteria identified by slide agglutination test

Answer 32

1-shigella | 2-salmonella

Question 33

Bacteria that detect in counter-immunoelectrophoresis test

Answer 33

That presen e in spinal fluid (detect capsular antigen) | -group B streptococci

Question 34

Enzyme-linked assay(ELISA)

Answer 34

To detect the homologous antigen | By bounded substrate into second antibody(eazyme)lead to color change

Question 35

Non treponemal test for syphilus detection

Answer 35

Cardiolpin-licithinncholestrol as antigen | VDR\RPR testes

Question 36

Use throat culture when:

Answer 36

- pharyngitis - diaphthrtia - thrush

Question 37

Ways to growth bacteria in sputum culture:

Answer 37

- blood agar - serilogical test - acid fast (mycobacterium) - antibody titer (mycoplasma) - anaerobic culture

Question 38

How we detect acute meningitis bacteria

Answer 38

By seriological test (immonolgical test) because they capsulated do quellung reaction

Question 38

Ways to growth bacteria in CSF

Answer 38

- serological test - gram stain - acid fast stain (need 6 weeks)