Lab1 (media)

Question 1

What is media?

Answer 1

Its solid or semisolid or lequid material support the bacteria growth

Question 2

Whats mateial use in solid and semisolid in past and now?

Answer 2

In past used (golatin material) thats distraction bacteria

Now used: sea wead material

Question 3

Ordinary culture media

Answer 3

• lequid :simple nutrient broth

- solid|semisolid:simple nutrient agar

Question 4

The media for fastidious bacteria

Answer 4

Enrichment broth in liquid

Enrichment agar in solid

Question 5

MacConky agar

Answer 5

Enriched Selective and differential media
Selective: by (bile salts)killed gram(-).
Differential: by (lactose fermentation) , if bacteria do fermentation(pink color) , if no (colorless).

Question 6

Blood agar

Answer 6

Sheep blood 5%

Differential agar , distinguished restriction blood bacteria

Question 7

Eosin methylene blue agar(EMB)

Answer 7

Selctive and differential agar
Selective: killed gram+ by eosin stain
Differential ; by coloured (green)bacteria that do lactose fermentation

Question 8

X (hemin)and V(Nad)factors

Answer 8

Enriched media contain blood/egg and X (hemin)and V(Nad)factors , which bacteria need them In red blood cell
For Non-cellular bacteria

Question 9

Thayermartin agar(VCN)

Answer 9

• selective agar
• contain veomyosis that killed antibiotics
• just for growth nesseria

Question 10

Candle jack agar

Answer 10

For anaerobic bacteria

By absorbed o2