Laboratory Exercise 9 Bacterial Anatomy

Question 1

Capsule Staining

Answer 1

Anthony’s Method - CVCS
Negative Staining - India Ink

• no heat fixing

Question 2

Anthony’s Method
CVCS

Answer 2

1% Crystal Violet - primary stain
20% Copper sulfate - decolorizer/counterstain

Question 3

India Ink
Other name

Answer 3

Nigrosine

Question 4

This ink does not adhere to organisms but they obliterate the background

Answer 4

India Ink

Question 5

Endospore Staining

Answer 5

Schaeffer-Fulton Method - MGHWS
Hansen’s Method - CAM

Question 6

Endospore Staining
Schaeffer-Fulton Method

Answer 6

Malachite Green + Heat
Water (decolorizer)
Safranin

green endospore, pink vegetative cell

Question 7

Endospore Staining
Hansen’s Method

Answer 7

Carbol fuchsin (5 mins)
Acetic acid (15 sec)
Methlylene blue (2 mins)

Question 8

Granules Staining

Answer 8

Gram Stain - CLAS

Question 9

Granules Staining
Gram Staining

Answer 9

Crystal Violet (1 min)
Lugol’s Iodine (1 min)
Acetone Alcohol (10 sec)
Safranin (30 sec)

Question 10

Demonstration of Flagella

Answer 10

Hanging Drop Presentation
SIM (Motility)

Question 11

Demonstration of Flagella
Hanging Drop Presentation

Answer 11

Broth culture on center of coverslip, do not spread drop, do not use oil immersion lens

Question 12

Demonstration of Flagella
SIM Method (Motility)

Answer 12

Proteus mirabilis +
SA -
EC +
B. subtilis +