Laboratory Diagnosis Flashcards
In viral antigen detection, what is the reagent that comes from rabbits, horses, chickens, etc?
antibodies
10-14 days; there is already resolution because you already developed antibodies
Covalescent
Fever and rashes are seen; you get a sample and then titer
Acute
Computes viral load
qPCR
True or false. Antibodies in the Viral Antigen Detection are usually monoclonal.
True
What microscope can let you view the structure of a virus
EM
What microscope that need to have the slides stained with immunostains so that ABS will bind to the virus
LM
Abnormal cells in viral culture
Continuous/transformed cell lines
Uses freshly explanted tissues from animal
Primary cell culture
In primary cell culture, cell are dissociated from one another by?
Trypsin
Specific pathogen free (SPF) live chicken eggs
Embryonated chicken eggs
-immortal
-often easier to grow than primary lines
-often aneuploid
cannot be used for vaccine production
Transformed cell lines
Normal primary cells are sometimes referred to as?
Diploid cells
Cells can be placed (or____) in a variety culture vessels
seeded
Primary cells will grow to confluence (aka ______), then stop dividing
monolayer
