Laboratory Diagnosis Flashcards
In viral antigen detection, what is the reagent that comes from rabbits, horses, chickens, etc?
antibodies
10-14 days; there is already resolution because you already developed antibodies
Covalescent
Fever and rashes are seen; you get a sample and then titer
Acute
Computes viral load
qPCR
True or false. Antibodies in the Viral Antigen Detection are usually monoclonal.
True
What microscope can let you view the structure of a virus
EM
What microscope that need to have the slides stained with immunostains so that ABS will bind to the virus
LM
Abnormal cells in viral culture
Continuous/transformed cell lines
Uses freshly explanted tissues from animal
Primary cell culture
In primary cell culture, cell are dissociated from one another by?
Trypsin
Specific pathogen free (SPF) live chicken eggs
Embryonated chicken eggs
-immortal
-often easier to grow than primary lines
-often aneuploid
cannot be used for vaccine production
Transformed cell lines
Normal primary cells are sometimes referred to as?
Diploid cells
Cells can be placed (or____) in a variety culture vessels
seeded
Primary cells will grow to confluence (aka ______), then stop dividing
monolayer
A common method of virus purification is precipitation with ________________ at 4° C overnight
polyethylene glycol
It requires known nucleic acid sequence for the virus
Real-Time PCR Detection of Viral Nucleic Acids
damages nucleic acids; crosslinks viral proteins
Radiation
inactivates some viruses
heat
preserves viruses
cold
renders some viruses susceptible to visible light
Photodynamic inactivation
amphipathic, thus solublizes membranes and can dissociate noncovalent bonds between viral proteins
Detergents
an organic solvent, thus damages envelop membranes of viruses
ether
cross-links nucleic acids and proteins
Formaldehyde
principal threats
Aerosols and punctures
allows one to count plaque forming units (pfu) as a quantitative measure of infectious virus
The plaque assay
is the unit used to describe the greatest dilution of a substance
Titer
The dilution that infects (or kills) 50% of cells is termed
tissue culture infectious dose-50 (TCID50)
Fluorescent Antibody Test (FAT)
• Radioimmunoassay (RAI)
• Enzyme-linked immunosorbent Assay(ELISA)
• Immunoelectron microscopy (IEM)
Microscopic Identification
Inclusion bodies
• Multinucleated giant cells
• TZANCK SMEAR
Light MICROSCOPY
approaches in the LABORATORY DIAGNOSIS OF VIRAL DISEASES
- Cell Culture
- Microscopic identification
- Serologic
- Viral antigen
- Viral nucleic acid
