Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Immunosero Lec (Finals) > Laboratory Detection of Bacterial Infections > Flashcards

Laboratory Detection of Bacterial Infections Flashcards

1
Q

Gold standard method in identifying bacterial infection as well as isolating it

A

Culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Traditional means of detencting bacterial agents

A

Bactrial culture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What are the 3 culture media

A

Enriched
Selective
Differential

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Media that inhibits growth of groups of organisms while facilitating the groups of another

A

Selective media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Allows to differentiate the use of bacteria based on their color characteristics

A

Differential agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

MacConkey agar is a what type of agar?

A

Selective and differential

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

2 types of stain used in microscopy?

A

Differential and Fluorescent

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Example of differential stain?

A

Gram
Acid fast
Giemsa

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Example of Fluorescent?

A

Fluorescein isothioscyanate

Auramine rhodamine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Limitation of fluoescent stain?

A

Training is needed

Not all organisms can be seen in this microscope

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Microscope that allows to see viral particles

A

Electron

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

The most widely known method for molecular?

A

PCR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Methhod of hoice for N. gonorrhoeae and C. trachomatis

A

Nucleic acid-based assay

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Highly specific and sensitivities have improved dramatically

A

Antigen detection

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Elisa

Latex agglutination

Lateral flow immunochromatography

Is an example of?

A

Antigen detection

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Detect and confirm infection from organisms that are difficult to grow

Useful in determining the causative agent when clinical manifestation are not specific enough

Disadvantage:
Delay between start of infection and antibody production

A

Antibody detection

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Major cause of bacterial pahryngitis and childhood impetigo

A

Group A strep (S. pyogenes)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Primary virulence factor for S. pyogenes?

A

M protein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Filamentous molecule consisting of 2 alpha-helical chains that extend out from the cell surface

A

M protein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Gene codes for M protein

A

emm genes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Virulence factor that:

Limits the depositio of C3 on the bacterial surface

A

M protein

22
Q

Virulence factor that:

Causes scarlet fever

A

Pyogenic exotoxins

23
Q

Strep throat and impetigo are the major sites of infection

T or F

A

T

24
Q

Due to infection with GAS that produces pyrogenic exotoxins

A

Scarlet fever

25
Q

Antibodies of diagnostic significance for GAS?

A

ASO

Anti-DNase

26
Q

Detects antibodies to the streptolysin O enzyme produced by GAS

A

Asntistreptolysin testing

27
Q

Clinically useful for patients suspected of having PSGN preceeded by
streptococcal skin infections

A

Anti-DNase B testing

28
Q

A slide agglutination screening test for the detection of antibodies against
streptococcal antigens

A

Streptozyme testing

29
Q

is oxygen labiled hemolysin produced by S. pyogenes

A

Streptolysin O

30
Q

Strepthroat usually negative in ASO?

T or F

A

T

31
Q

Gram-negative, microaerophilic, helical rods

A

Helicobacter pylori

32
Q

Major cause of gastric and duodenal ulcer

A

Helicobacter pylori

33
Q

MOT or H. pylori?

A

Fecal-oral route

34
Q

Major virulence factor for H. pylori

A

Protein CagA

35
Q

Detection of H. pylori Ag before & after antimicrobial therapy in fecal
samples help determine if bacteria has been eliminated following
treatment

A

Fecal Ag test

36
Q

Smallest free living organisms that can grow on artificial media with no cell wall

A

Mycoplasma pneumoniae

37
Q

MOT for M. pneumoniae

A

Droplets

38
Q

Inc pd for M. pneumoniae

A

1-3 weeks

39
Q

Dermatological manifestation of mycoplasma pneumoniae?

A

Steven-Johnson syndrome

Reynaud Syndrome

40
Q

M. pneumoniae is a fasidious microorganisms

T or F?

A

T

41
Q

Appearane in culture for H. pylori?

A

Fried egg/hockey puck

42
Q

Gram (-) obligate intracellular organism

A

Rickettsia

43
Q

MOT for Rickettsia?

A

Tick borne

44
Q

Rickettsia is Can grow in MacConkey?

T or F

A

F

Even though it is Gram neg, it cannot survive in artificial media

45
Q

Term when infant ticks up to adulthood, meron syang rickettsia

A

Transstadial

46
Q

Term whenl parang vertical transmission ng Rickettsia pero for
ticks (mother tick to child tick)

A

Transovarial

47
Q

causative agent for Rocky Mountain Spotted Fever?

A

Rickettsia rickettsii

48
Q

Gold standard for Rickettssia?

A

Indirect IFA

49
Q

Ox-19 - (+)
Ox-2 - (+)
Ox-K - (-)

A

prowazeki
typhi
rickettsii

50
Q

Ox-19 - (-)
Ox-2 - (-)
Ox-K - (-)

A

akari

Coxiella burnetti

51
Q

Ox-19 - (-)
Ox-2 - (-)
Ox-K - (+)

A

Orientia tsutsugamushi

Immunosero Lec (Finals) (5 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions