Laboratory biosafety and biosecurity Flashcards
The containment principles, technologies, and practices that are implemented to prevent unintentional exposure to pathogens and toxin.
Protects the people from the pathogens
Biosafety
The protection, control, and accountability for valuable biological materials within laboratories, in order to prevent their unauthorized access, loss, theft, misuse, diversion, or intentional release
Protects the pathogens from the people
Biosecurity
Microorganisms that are unlikely to cause human or animal disease
Risk Group 1
Risk group 1
Low individual risk, low community risk
Microorganisms that are unlikely to be a significant risk to laboratory workers and the community, livestock, or the environment.
Laboratory exposure may cause infection
Risk group 2
Risk group 2
Moderate individual risk, Limited community risk
Risk group 3
High individual risk, Limited to moderate community risk
Microorganisms that are known to cause serious disease to humans or animals and may present significant risk to lab workers
Risk group 3
Risk group 4
High individual risk, High community risk
-Microorganisms that are known to produce life-threatening disease to humans or animals
-Represents a significant risk to laboratory workers.
Risk group 4
under what biosafety level are bacillus subtilis, naegleria gruberi, infectious canine hepatitis virus?
Biosafety level 1
Suitable for work involving microorganisms that are defined and well-characterized strains known not to cause disease in humans
Most appropriate among undergraduate and secondary educational training and teaching laboratories
Biosafety level 1
under what biosafety level are hepatitis B virus, Salmonella species, and toxoplasma species?
Biosafety level 2
Laboratories that deal with indigenous moderate-risk agents present in the community
Observes practices, equipment, and facility design that are applicable to clinical, diagnostic, and teaching laboratories consequently observing good microbiological techniques
Biosafety level 2
Under what biosafety level are mycobacterium tuberculosis, St, Louis encephalitis virus, and Coxiella?
Biosafety level 3
Emphasis on primary and secondary barriers in the protection of the personnel, the community, and the environment from infectious aerosol exposure
Work with indigenous or exotic agents with a potential for respiratory transmission, and that may cause serious and potentially lethal infection are being conducted in this level
Biosafety level 3
Required for work with dangerous and exotic agents that poses high individual risk of life-threatening diseases that may be transmitted via the aerosol route, for which there are no available vaccines of treatment
Specific practices, safety equipment, and appropriate facility design and construction are required
Biosafety level 4
Under what biosafety level are Marburg virus, Cream-Congo hemorrhagic fever
Biosafety Level 4
Risk associated to biological toxins or infectious agents
Biorisk
Integration of biosafety and biosecurity to manage risks when working with biological toxins and infectious agents
CWA 15793 Laboratory Biorisk Management standard
A system or process to control safety and security risks associated with the handling or storage and disposal of biological agents and toxins in laboratories and facilities
CEN Workshop Agreement 15793:2011:
Three components of biorisk management
Assessment
Mitigation
Performance
Anything in the environment that has the potential to cause harm
Hazard
Possibility that something bad or unpleasant will happen
Risk
For risk to occur, there must be a _________ for the hazard to cause harm
Situation
Risk assessment process
Define the situation
Define the risks
Characterize the risks
Determine if risks are acceptable or not
Actions and control measures that are put into place to reduce or eliminate the risks associated with biological agents and toxins
Mitigation procedures
Hierarchy of Controls; from most effective to the least effective:
Elimination
Substitution
Engineering controls
Administrative controls
PPE
Most difficult and most effective control measure
Total decision of not to work with a specific biological agent or even not doing the intended work
Elimination
Replacement of the procedures or biological agent with a similar entity in order to reduce the risk
Substitution
Physical changes in workstations, equipment, production facilities or any other relevant aspect of the work environment that can reduce or prevent exposure to hazards
Engineering Controls
Policies, standards, and guidelines used to control risks
Practices and procedures comprise minimizing splashes, sprays, and aerosols to avoid laboratory-acquired infections or following standard operating procedures
Administrative controls
Devices were worn by workers to protect them against chemicals, toxins, and pathogenic hazards in the laboratory
Least effective measure as it only protects the person who is wearing it, and only when it is used correctly
Personal Protective Equipment
