Laboratory Activity 12 – Rheumatoid Factor Determination

Question 1

is a chronic disease that affects the joints characterized by inflammation, stiffness, and soreness

Answer 1

Rheumatoidarthritis (RA)

Question 2

Most individuals affected experience a gradual onset of the disease, but usually, all joints in the body will be affected

Answer 2

rheumatoid arthritis

Question 3

As the disease progresses, other organs of the body can develop an inflammatory condition

Answer 3

rheumatoid arthritis

Question 4

determinatio is essential in the prognosis and therapeutic management of rheumatoid arthritis

Answer 4

rheumatoid factor

Question 5

hyperensitivity; autoimmune

Answer 5

Autoimmune

Question 6

An anti-antibody that binds to the Fc portion of abnormal IgG

Question 7

Associated to rheumatoid arthritis

Question 8

Chronic systemic inflammatory disorder in which joint cartilage, ligaments, and tendons are destroyed

Question 9

An autoimmune disease that affects the synovial membrane of multiple joints

Question 10

Also noted in chronic hepatitis, syphilis, (SLE), scleroderma, Sjogren’s syndrome, and B-cell lymphoproliferative disorders

Question 11

specific marker; serologic marker; anti-antibody IgM against altered IgG molecules

Question 12

not present in 25%

Question 13

suggests presence of RA

Question 14

inflammation of the knees

Question 15

bacteria affecting kneees
macrophages
IgE

Question 16

-new markers; antigen (nonself)

Answer 16

neoantigens

Question 17

can be employed to test for the presence of the antibody, using a carrier particle such as sheep erythrocytes sensitized with IgG

Answer 17

Passive agglutination

Question 18

When patient serum containing RF is mixed with the sensitized reagent cells, [?] occurs

Answer 18

visible agglutination

Question 19

Latex fixation test by

Answer 19

Singer and Plotz

Question 20

Latex agglutination titer lower limit based on an undiluted sample and diluted sample at

Answer 20

1:10

Question 21

•Very low titer: SLE, endocarditis, tuberculosis, syphilis, viral infection, and diseases of the liver, lung, or kidney
•Low titer: approximately 1 percent of healthy individuals

Answer 21

(+) undiluted

Question 22

RF is present in the specimen at a level generally associated with RA

Answer 22

(+) diluted at 1:10

Question 23

Interpretation of results based on titer:
Positive: ____________________
Weakly positive: ____________________
Negative: ____________________

Answer 23

Positive: > or = 80
Weakly positive: 20 to 40
Negative: 1:20

Question 24

Rose-Waaler Test

Answer 24

Sheep cell agglutination test

Question 25

Cohen et.al

Answer 25

Sensitized alligator erythrocyte test

Question 26

Bloch and Bunim

Answer 26

Bentonite flocculation test