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Immunology and Serology [LAB] > Laboratory Activities 15 and 16 – Determination of HBsAg and Anti-HBs > Flashcards

Laboratory Activities 15 and 16 – Determination of HBsAg and Anti-HBs Flashcards

1
Q

HAV antibodies

A

IgM Anti-HAV
IgG Anti-HAV

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2
Q

HBV Antigens

A

HBsAg
HBeAg
HBcAg

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3
Q

HBV Antibodies

A

Anti-HBs
Anti-HBe
Anti-HBc

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4
Q

Anti-HBc sub Ab

A

IgM Anti-HBc
IgG Anti-HBc/ Anti-HBc Total

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5
Q

HEV Antibodies

A

IgM anti-HEV

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6
Q

Shed in the feces:
-incubation period
-early acute stage

A

HAV antigens

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7
Q

decline of low levels:
-symptoms appear

A

HAV antigens

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8
Q

Not a clinically useful indicator of disease

A

HAV antigens

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9
Q

1 - enzyme immunoassay (EIA), radioimmunoassay (RIA) methods

2 - ELISA (solid-phase antibody-capture)

3 - ELISA (competitive inhibition)

4 - biopsy of infected liver

A

1 - IgM Anti-HAV, IgG Anti-HAV

2 - IgM Anti-HAV

3 - IgG Anti-HAV

4 - HBcAg

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10
Q
  • 1 month: peak of illness (acute)
  • 3 to 12 months: after the onset of the disease
  • 6 to 12 months: decline to undetectable levels
A

IgM anti-HAV

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11
Q

Marker of acute hepatitis A

A

IgM Anti-HAV

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12
Q

Produced as a result of natural infection or immunization (immunity to HAV)

A

IgG Anti-HAV

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13
Q

IgM titers and IgG anti-HAV antibodies relationship

A

Inversely proportional

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14
Q

First marker of hepatitis B

A

HBsAg

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15
Q

Indicator of HBV active infection

A

HBsAg

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16
Q

Important HBV marker for screening blood donors

A

HBsAg

17
Q
  • 1 week to 2 months: after exposure
  • 2 weeks to 2 months: before the onset of the disease
  • convalescent phase of acute or chronic infection with unresolved antigenemia: decline to undetectable levels
A

HBsAg

18
Q
  • 2 weeks: after exposure (appearance of the surface antigens)
  • 2 weeks: before the onset of the disease
  • 2 weeks: decline to undetectable levels
A

HBeAg

19
Q

Present during periods of active replication of the virus

A

HBeAg

20
Q

Indicates a high degree of infectivity if present

A

HBeAg

21
Q

Not detectable in serum because of the viral envelope that masks it

A

HBcAg

22
Q
  • shortly: after exposure (appearance of the surface antigens) - ALT levels begin to rise
A

Anti-HBc

Immunology and Serology [LAB] (16 decks)

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