Laboratory Activities 15 and 16 – Determination of HBsAg and Anti-HBs Flashcards
HAV antibodies
IgM Anti-HAV
IgG Anti-HAV
HBV Antigens
HBsAg
HBeAg
HBcAg
HBV Antibodies
Anti-HBs
Anti-HBe
Anti-HBc
Anti-HBc sub Ab
IgM Anti-HBc
IgG Anti-HBc/ Anti-HBc Total
HEV Antibodies
IgM anti-HEV
Shed in the feces:
-incubation period
-early acute stage
HAV antigens
decline of low levels:
-symptoms appear
HAV antigens
Not a clinically useful indicator of disease
HAV antigens
1 - enzyme immunoassay (EIA), radioimmunoassay (RIA) methods
2 - ELISA (solid-phase antibody-capture)
3 - ELISA (competitive inhibition)
4 - biopsy of infected liver
1 - IgM Anti-HAV, IgG Anti-HAV
2 - IgM Anti-HAV
3 - IgG Anti-HAV
4 - HBcAg
- 1 month: peak of illness (acute)
- 3 to 12 months: after the onset of the disease
- 6 to 12 months: decline to undetectable levels
IgM anti-HAV
Marker of acute hepatitis A
IgM Anti-HAV
Produced as a result of natural infection or immunization (immunity to HAV)
IgG Anti-HAV
IgM titers and IgG anti-HAV antibodies relationship
Inversely proportional
First marker of hepatitis B
HBsAg
Indicator of HBV active infection
HBsAg
Important HBV marker for screening blood donors
HBsAg
- 1 week to 2 months: after exposure
- 2 weeks to 2 months: before the onset of the disease
- convalescent phase of acute or chronic infection with unresolved antigenemia: decline to undetectable levels
HBsAg
- 2 weeks: after exposure (appearance of the surface antigens)
- 2 weeks: before the onset of the disease
- 2 weeks: decline to undetectable levels
HBeAg
Present during periods of active replication of the virus
HBeAg
Indicates a high degree of infectivity if present
HBeAg
Not detectable in serum because of the viral envelope that masks it
HBcAg
- shortly: after exposure (appearance of the surface antigens) - ALT levels begin to rise
Anti-HBc
