Lab Session 2-5 Flashcards
Magnifies images ten times
Ocular lens
Which lens has four different lenses
Objective
100x is also known as
Immersion lens
Only use lens paper to wipe surfaces
Ocular lens
Use lens cleaning fluid and lens paper
Objective lens
Pathogenic
Disease causing
Clean workbench with
10% bleach
Works best when near its highest position
Condenser
True or false
Never move the condenser to decrease the amount of light coming through the microscope
True
Switch objectives but the image should stay nearly in focus and only require minor adjustments with the fine focus
Parfocal
When switching from 10x to 100x objective
Turn the fine focus to refocus the image
We will rarely use
40x objective
Always find an area of interest with 10x objective and then center it and switch to 100x objective
True
Total magnification
1000x
Ability to make small objects seem larger
Magnification
Allows us to see fine details
Resolution
Focuses light onto specimen
Purpose of condenser
Minimize the loss of light
Oil immersion lens
Why invert plates when incubating them
To prevent contamination and condensation
Minimizes contamination by microorganisms floating in the air
Aseptic technique
Why label each plate on the bottom
So you know it’s yours and what it contains
Mass of cells
Colony
Cfu
Colony forming units
Single microbial cell that gave bacteria to colony