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Microbiology > Lab Quiz 3 (Lab 5 & 6) > Flashcards

Lab Quiz 3 (Lab 5 & 6) Flashcards

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Biology 101 flashcards
1
Q

The ____ test tests for the enzyme catalase

Drop ____ on the bacteria to perform the test

Bubbles = Positive

This test cannot be performed on bacteria on blood agar because blood contains catalease and a false positive may occur

A

Catalease

3% hydrogen peroxide

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2
Q

The top catalase test is a ____

A

Positive

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3
Q

The ____ tests for the conversion of nitrate or to ammonia or nitrogen

A

Nitrate

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4
Q

The Nitrate test uses ____ as its medium

The Nitrate test uses ____ and ____ as its reagents

A

Nitrate broth

Nitrate Reagent A & Nitrate Reagent B

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5
Q

In the Nitrate test, you first:

  1. Add 5 drops of ____ and ____
  2. Look for red color
  3. If no red color, add ____
  4. Look for red color
A
  1. Nitrate Reagent A & Nitrate Reagent B
  2. Zinc dust
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6
Q

In the Nitrate test, red color after the addition of nitrate reagents indicates the bacteria have changed the nitrate to ____

No color change after the addition of zinc indidates the bacteria have converted nitrates to ____ or nitrogen gas

A

nitrite

ammonia

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7
Q

The Mannitol Salt Agar (MSA) is selective for ____ such as Staphylococcus

A

Halophiles

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8
Q

With the Mannitol Salt Agar (MSA) test, you must report two results: ____ & ____

A

Growth and Fermentation

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9
Q

With the Mannitol Salt Agar (MSA) test, you streak for ____

A

Isolation

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10
Q

This MSA test reports

A

No Growth (NG)

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11
Q

This MSA test reports

A

Growth with No Fermentation (G-)

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12
Q

This MSA test reports

A

Growth with Fermentation (G+)

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13
Q

The ____ Test uses Bacitracin, which is a peptide antibiotic that inhibits cell wall synthesis in a susceptible bacteria

A

Bacitracin Sensitivity

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14
Q

The Bacitracin Sensitivity uses an ____ plate as the medium

A

SBA

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15
Q

In the Bacitracin Sensitivity Test, sensitive bacteria will have a ____, resistent bacteria will not

A

zone of inhibition

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16
Q

According to the Bacitracin Sensitivity Test, this bacteria is:

A

Sensitive

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17
Q

According to the Bacitracin Sensitivity Test, this bacteria is:

A

Resistent

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18
Q

In the Novobiocin test, sensitive bacteria will have a ____. Resistent bacteria will not.

A

Zone of Inhibition

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19
Q

In the Novobiocin test, you swap an ____ plate and place down novobiocin disks using sterible forceps

A

SBA

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20
Q

In the Novobiocin test on the left is

A

Resistent

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21
Q

In the Novobiocin test on the right is

A

Sensitive

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22
Q

The Coagulase test on the top is ____

A

Positive

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23
Q

The Coagulase test on the bottom is ____

A

Negative

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24
Q

The ____ test tests for the production of the enzyme coagulase

It is also used to differentate between staphylococcus species

A

Coagulase

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25
Q

To perform a Coagulase test, you first add bacteria to coagulase ____ (from rabbits)

Incubate

Look for coagulation of the plasma

A

plasma

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26
Q

A positive result in the Coagulase test is ____

A

Coagulation

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27
Q

With the Hemolysis on Sheep’s Blood Agar Test, you first:

Streak for ____

Incubate

Observe hemolysis by holding the plate up to a light

A

isolation

28
Q

____ hemolysis is complete hemolysis and will result in a clear zone around the colonies

A

Beta

29
Q

This is ____ hemolysis

A

Beta

30
Q

____ hemolysis is partial hemolysis and will result in a greenish zone around the colonies

A

Alpha

31
Q

This is ____ hemolysis

A

Alpha

32
Q

____ hemolysis is no hemolysis and is indicated by no zone around the colonies

A

Gamma

33
Q

This is ____ hemolysis

A

Gamma

34
Q

The Voges-Prokauer Test tests for the production of a ____ end product from fermentation

The Voges-Prokauer Test uses ____ as a medium

A

neutral

MR-VP Broth

35
Q

Steps of the Voges-Prokauer Test

  1. Innoculate ____ broth. Incubate for 48 hours
  2. Add ____, ____, and ____
  3. Incubate at room temperature and look for a ____ color
A
  1. MR-VP
  2. VP Reagent A, VP Reagent B, Creatine
  3. Red
36
Q

The VP Test on the left is ____

A

positive

37
Q

The VP Test on the right is ____

A

negative

38
Q

The Growth in 6.5% NaCl broth test is selective for ____

A

halophiles

39
Q

NaCl broth test

The tube on the left is ____

A

Positive

40
Q

Growth in 6.5% NaCl broth test

A positive will have growth in the tube, which will appear as ____

A

turbidity

41
Q

In the Bible Esculin test, ____ is your medium

A

Bile Esculin agar slant

42
Q

the Bible Esculin test inhibits the growth of other ____ organisms

A

Gram +

43
Q

Bible Esculin Test

The tube in the middle is ____

A

Negative

44
Q

Bible Esculin Test

The tube on the right is ____

A

Positive

45
Q

With the Bile Esulin test, hydrolysis of esculin causes a black ____ and should be reported as + (black) or - (not black)

A

precipitate

46
Q

The ____ Test tests for the production of amalyase in bacteria

A

Starch Hydrolysis

47
Q

Starch Hydrolysis Test

  1. Inoculate the organism by streaking a single line down the center of a ____ plate
  2. Incubate at 37°C
  3. Flood the plate with ____
  4. Let the plate sit for a couple of minutes and pour the solution down the sink
  5. Observe the plate for a ____ around the bacteria
A
  1. Starch Agar
  2. Iodine
  3. Clear zone
48
Q

Starch Hydrolysis Test

Iodine reacts with starch in the plate and turns it a dark purple/black color. A clear zone around the bacteria indicates the search has been ____ and is a ____ result

A

Hydrolyzed; Positive

49
Q

The ____ Test is useful in identifying Streptococcus pneumonia. The growth of S. pneumonia is inhibited by it.

A

Optochin Sensitivity

50
Q

The Optochin Sensistivity Test steps:

  1. Swab an ____ plate with bacteria in three directions
  2. Using sterile forceps, place a ____ on the plate
A
  1. SBA
  2. optichin disk
51
Q

The Sulfur Indole Mobility Test (SIM) can be used to operform three separate tests in one tube.

  1. Tests for the production of ____
  2. Conversion of tryptophan to ____
  3. Ability to move through semi-solid agar due to the presence of ____ (mobility test)
A
  1. H2S
  2. indole
  3. flagella
52
Q

Steps of the Sulfur Indole Mobility Test (SIM)

  1. Stab a ____ deep
  2. Incubate

For mobility test: Look for growth of bacteria at sites distant from the initial point of inoculation

For H2S production: Look for a ____ precipitate

For Indole test: Add a layer of ____ reagent and look for a ____ color

A
  1. SIM

For mobility test: Look for growth of bacteria at sites distant from the initial point of inoculation

For H2S production: Look for a black precipitate

For Indole test: Add a layer of Kovac’s reagent and look for a red color

53
Q

Sulfur Indole Mobility Test (SIM)

Mobility potion

The tube on the left is ____

The tube in the middle is ____

The tube on the right is ____

A

Negative

Positive

Positive

54
Q

Sulfur Indole Mobility Test (SIM)

Indole potion

The tube on the left is ____

The tube on the right is ____

A

Negative

Positive

55
Q

Sulfur Indole Mobility Test (SIM)

H2S Production potion

The tube on the left is ____

The tube on the right is ____

A

Negative

Positive

56
Q

The Mannose Fermentation tests detects mannose using ____, which contains phenol red as an indicator

A

Cystine Tryptic Agar supplemented with mannose (CTA-M)

57
Q

The Mannose Fermentation tests detects mannose using Cystine Tryptic Agar supplemented with mannose (CTA-M), which contains ____ as an indicator

A

phenol red

58
Q

Steps of the Mannose Fermentation test

  1. Inoculate ____ by stabbing with a inoculating needle
  2. Incubate
  3. Look for a ____ color and for ____, ____
A
  1. CTA-M
  2. yellow; cracks; bubbles
59
Q

____ Test tests for the hydrolysis of casein

A

The Casein Agar (Skim Milk Agar)

60
Q

Steps for The Casein Agar (Skim Milk Agar) Test

  1. Streak a single line down the center of the ____
  2. Incubate
  3. Observe plates for a ____ around the line of bacteria
A
  1. Casein Agar plate
  2. Clear zone
61
Q

The Casein Agae (Skim Milk Agar) Test

The line on the left is a ____

The line on the right is a ____

A

Negative

Positive

62
Q

The ____ tests for the production of acidic end products from fermentation

A

Methyl Red Test

63
Q

Steps of the Methyl Red Test

  1. Inoculate ____
  2. Incubate
  3. Add ____
  4. Mix
  5. Look for ____ color
A
  1. MR-VP broth
  2. Methyl Red
  3. Red
64
Q

The Methyl Red Test

The tube on the left is ____

The tube on the right is ____

A

Negative

Positive

65
Q

OPNG Test

Some bacteria produce an enzyme, ____, which can hydrolyze OPNG into galactose and ortho-nitrophenol. The ortho-nitrophenol has a ____ color.

A

β-galatosidase; yellow

66
Q

Steps of the ONPG test

  1. Inoculate ____ using a heavy inoculum
  2. Using sterile forceps, place a disk of ____ into the tube
  3. Incubate for 24 hours
  4. Look for a ____ color
A
  1. ONPG broth
  2. ONPG reagent
  3. yellow
67
Q

OPNG test

The tube on the left is ____

The tube on the right is ____

A

Negative

Positive

Microbiology (9 decks)

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