Lab Quiz 3 (Lab 5 & 6) Flashcards
The ____ test tests for the enzyme catalase
Drop ____ on the bacteria to perform the test
Bubbles = Positive
This test cannot be performed on bacteria on blood agar because blood contains catalease and a false positive may occur
Catalease
3% hydrogen peroxide
The top catalase test is a ____
Positive
The ____ tests for the conversion of nitrate or to ammonia or nitrogen
Nitrate
The Nitrate test uses ____ as its medium
The Nitrate test uses ____ and ____ as its reagents
Nitrate broth
Nitrate Reagent A & Nitrate Reagent B
In the Nitrate test, you first:
- Add 5 drops of ____ and ____
- Look for red color
- If no red color, add ____
- Look for red color
- Nitrate Reagent A & Nitrate Reagent B
- Zinc dust
In the Nitrate test, red color after the addition of nitrate reagents indicates the bacteria have changed the nitrate to ____
No color change after the addition of zinc indidates the bacteria have converted nitrates to ____ or nitrogen gas
nitrite
ammonia
The Mannitol Salt Agar (MSA) is selective for ____ such as Staphylococcus
Halophiles
With the Mannitol Salt Agar (MSA) test, you must report two results: ____ & ____
Growth and Fermentation
With the Mannitol Salt Agar (MSA) test, you streak for ____
Isolation
This MSA test reports
No Growth (NG)
This MSA test reports
Growth with No Fermentation (G-)
This MSA test reports
Growth with Fermentation (G+)
The ____ Test uses Bacitracin, which is a peptide antibiotic that inhibits cell wall synthesis in a susceptible bacteria
Bacitracin Sensitivity
The Bacitracin Sensitivity uses an ____ plate as the medium
SBA
In the Bacitracin Sensitivity Test, sensitive bacteria will have a ____, resistent bacteria will not
zone of inhibition
According to the Bacitracin Sensitivity Test, this bacteria is:
Sensitive
According to the Bacitracin Sensitivity Test, this bacteria is:
Resistent
In the Novobiocin test, sensitive bacteria will have a ____. Resistent bacteria will not.
Zone of Inhibition
In the Novobiocin test, you swap an ____ plate and place down novobiocin disks using sterible forceps
SBA
In the Novobiocin test on the left is
Resistent
In the Novobiocin test on the right is
Sensitive
The Coagulase test on the top is ____
Positive
The Coagulase test on the bottom is ____
Negative
The ____ test tests for the production of the enzyme coagulase
It is also used to differentate between staphylococcus species
Coagulase
To perform a Coagulase test, you first add bacteria to coagulase ____ (from rabbits)
Incubate
Look for coagulation of the plasma
plasma
A positive result in the Coagulase test is ____
Coagulation
With the Hemolysis on Sheep’s Blood Agar Test, you first:
Streak for ____
Incubate
Observe hemolysis by holding the plate up to a light
isolation
____ hemolysis is complete hemolysis and will result in a clear zone around the colonies
Beta
This is ____ hemolysis
Beta
____ hemolysis is partial hemolysis and will result in a greenish zone around the colonies
Alpha
This is ____ hemolysis
Alpha
____ hemolysis is no hemolysis and is indicated by no zone around the colonies
Gamma
This is ____ hemolysis
Gamma
The Voges-Prokauer Test tests for the production of a ____ end product from fermentation
The Voges-Prokauer Test uses ____ as a medium
neutral
MR-VP Broth
Steps of the Voges-Prokauer Test
- Innoculate ____ broth. Incubate for 48 hours
- Add ____, ____, and ____
- Incubate at room temperature and look for a ____ color
- MR-VP
- VP Reagent A, VP Reagent B, Creatine
- Red
The VP Test on the left is ____
positive
The VP Test on the right is ____
negative
The Growth in 6.5% NaCl broth test is selective for ____
halophiles
NaCl broth test
The tube on the left is ____
Positive
Growth in 6.5% NaCl broth test
A positive will have growth in the tube, which will appear as ____
turbidity
In the Bible Esculin test, ____ is your medium
Bile Esculin agar slant
the Bible Esculin test inhibits the growth of other ____ organisms
Gram +
Bible Esculin Test
The tube in the middle is ____
Negative
Bible Esculin Test
The tube on the right is ____
Positive
With the Bile Esulin test, hydrolysis of esculin causes a black ____ and should be reported as + (black) or - (not black)
precipitate
The ____ Test tests for the production of amalyase in bacteria
Starch Hydrolysis
Starch Hydrolysis Test
- Inoculate the organism by streaking a single line down the center of a ____ plate
- Incubate at 37°C
- Flood the plate with ____
- Let the plate sit for a couple of minutes and pour the solution down the sink
- Observe the plate for a ____ around the bacteria
- Starch Agar
- Iodine
- Clear zone
Starch Hydrolysis Test
Iodine reacts with starch in the plate and turns it a dark purple/black color. A clear zone around the bacteria indicates the search has been ____ and is a ____ result
Hydrolyzed; Positive
The ____ Test is useful in identifying Streptococcus pneumonia. The growth of S. pneumonia is inhibited by it.
Optochin Sensitivity
The Optochin Sensistivity Test steps:
- Swab an ____ plate with bacteria in three directions
- Using sterile forceps, place a ____ on the plate
- SBA
- optichin disk
The Sulfur Indole Mobility Test (SIM) can be used to operform three separate tests in one tube.
- Tests for the production of ____
- Conversion of tryptophan to ____
- Ability to move through semi-solid agar due to the presence of ____ (mobility test)
- H2S
- indole
- flagella
Steps of the Sulfur Indole Mobility Test (SIM)
- Stab a ____ deep
- Incubate
For mobility test: Look for growth of bacteria at sites distant from the initial point of inoculation
For H2S production: Look for a ____ precipitate
For Indole test: Add a layer of ____ reagent and look for a ____ color
- SIM
For mobility test: Look for growth of bacteria at sites distant from the initial point of inoculation
For H2S production: Look for a black precipitate
For Indole test: Add a layer of Kovac’s reagent and look for a red color
Sulfur Indole Mobility Test (SIM)
Mobility potion
The tube on the left is ____
The tube in the middle is ____
The tube on the right is ____
Negative
Positive
Positive
Sulfur Indole Mobility Test (SIM)
Indole potion
The tube on the left is ____
The tube on the right is ____
Negative
Positive
Sulfur Indole Mobility Test (SIM)
H2S Production potion
The tube on the left is ____
The tube on the right is ____
Negative
Positive
The Mannose Fermentation tests detects mannose using ____, which contains phenol red as an indicator
Cystine Tryptic Agar supplemented with mannose (CTA-M)
The Mannose Fermentation tests detects mannose using Cystine Tryptic Agar supplemented with mannose (CTA-M), which contains ____ as an indicator
phenol red
Steps of the Mannose Fermentation test
- Inoculate ____ by stabbing with a inoculating needle
- Incubate
- Look for a ____ color and for ____, ____
- CTA-M
- yellow; cracks; bubbles
____ Test tests for the hydrolysis of casein
The Casein Agar (Skim Milk Agar)
Steps for The Casein Agar (Skim Milk Agar) Test
- Streak a single line down the center of the ____
- Incubate
- Observe plates for a ____ around the line of bacteria
- Casein Agar plate
- Clear zone
The Casein Agae (Skim Milk Agar) Test
The line on the left is a ____
The line on the right is a ____
Negative
Positive
The ____ tests for the production of acidic end products from fermentation
Methyl Red Test
Steps of the Methyl Red Test
- Inoculate ____
- Incubate
- Add ____
- Mix
- Look for ____ color
- MR-VP broth
- Methyl Red
- Red
The Methyl Red Test
The tube on the left is ____
The tube on the right is ____
Negative
Positive
OPNG Test
Some bacteria produce an enzyme, ____, which can hydrolyze OPNG into galactose and ortho-nitrophenol. The ortho-nitrophenol has a ____ color.
β-galatosidase; yellow
Steps of the ONPG test
- Inoculate ____ using a heavy inoculum
- Using sterile forceps, place a disk of ____ into the tube
- Incubate for 24 hours
- Look for a ____ color
- ONPG broth
- ONPG reagent
- yellow
OPNG test
The tube on the left is ____
The tube on the right is ____
Negative
Positive
