Lab Quiz 2 (Lab 3 & 4)

Question 1

____ - The process of putting bacteria on a microscope slide for staining

Answer 1

Preparing a bacterial smear

Question 2

When preparing a smear from a solid culture medium, a ____ is used to spread some of the bacteria into a drop of water on the microscope slide

Answer 2

Inoculating loop

Question 3

When preparing a smear from a broth culture, the ____ is not needed

Answer 3

drop of water

Question 4

The concentration of bacteria in ____ is usually much lower that can be achieved by putting bacteria from a plate into a drop of water

Answer 4

broth

Question 5

____ adheres the bacteria to the glass of the microscope slide so that they are not washed off in the subsequent staining steps

Answer 5

Fixing

Question 6

A ____ stain used a single colored dye

Answer 6

Simple

Question 7

All bacteria will stain the same color using a ____ stain

Answer 7

simple

Question 8

____ stain is useful when you want to see the shape or arrangement of bacteria

Answer 8

simple

Question 9

In a ____ stain, an acidic dye is used to release H+ ions

Answer 9

negative

Question 10

In a ____ stain, the stain adheres to the microscope slide around the bacteria

Answer 10

negative

Question 11

In a ____ stain, the bacteria appear as white dots against a dark gray to black background

Answer 11

negative

Question 12

A ____ stain used to determine the presence and position of endospores within or associated with cells of certain gram-positive bacteria

Answer 12

spore

Question 13

In a spore stain, you use ____ as the stain

Answer 13

Malachite green

Question 14

In a ____ stain, you place the slide over a steam bath

Answer 14

spore

Question 15

The steps of a gram stain

Answer 15

1. Primary stain (Crystal Violet)
2. Mordant (Iodine)
3. Decolorizer (Alcohol)
4. Safranin

Question 16

The ____ stain indicates the presence of the waxy type of lipid, mycolic acid

Answer 16

Acid fast

Question 17

In an Acid Fast stain, ____ penetrates the cell wall, staining them a reddish-purple color

Answer 17

Carbolfuchsin

Question 18

Acid Fast cells are ____, non-acid-fast cells are ____

Answer 18

Red; Blue

Question 19

Bacillus Subtilis has ____

Answer 19

spores

Question 20

Mycobacterium is ____

Answer 20

Acid Fast

Question 21

The microbiota on the human body used to be referred to as ____, but microbiologists are trying to stop using this term since it refers to plant life and bacteria are not plants

Answer 21

normal flora

Question 22

____, ____, and ____ are all sterile sites

Answer 22

Blood; The central nervous system; The major organs

Question 23

Sterile sites are only colonized by microorganisms during ____

Answer 23

disease

Question 24

Antibiotics can be ____, meaning they kill the bacteria

Answer 24

Bactericidal

Question 25

Antibiotics can be ____, meaning they inhibit the growth of bacteria enough that the patient’s immune system can control the infection

Answer 25

Bacteriostatic

Question 26

You can find both ____ and ____ microorganisms on your skin

Answer 26

Resident; Transient

Question 27

____ microbes are always on your skin

Answer 27

Resident

Question 28

____ organisms have been picked up from objects with which the skin has recently come into contact

Answer 28

Transient

Question 29

____ organisms are present on the skin in low numbers and for only a short period of time

Answer 29

Transient

Question 30

Most bacteria found on the skin as normal flora are gram ____ organisms

Answer 30

positive

Question 31

S. pyogenes is _-hemolytic

Answer 31

β

Question 32

All normal throat flora are _ or _ hemolytic

Answer 32

α or γ

Question 33

We used ____ as a medium to test urine

Answer 33

Uricult

Question 34

A urine sample containing less than 10,000 CFU/ml is considered ____ for a urinary tract infection

Answer 34

negative (normal)

Question 35

A urine sample containing 10,000-100,000 CFU/ml is considered ____ for a urinary tract infection

Answer 35

doubtful

Question 36

A urine sample containing greater than 100,000 CFU/ml is considered ____ for a urinary tract infection

Answer 36

positive

Question 37

The pale green side of the Uricult paddle contains ____ + ____. This medium prevents swarming of Proteus, a common urinary tract pathogen. It also changes to a yellowish color when Lactose is fermented.

Answer 37

CLED + Polymyxin

Question 38

The ____ tests for susceptibility of microorganisms to antibiotics using a disk-diffusion technique

Answer 38

Kirby-Bauer technique

Question 39

The ____ is a circular area around the spot of the antibiotic in which the bacteria colonies do not grow.

Answer 39

Zone of inhibition

Question 40

A large zone of inhibition means the bacteria is ____ to the antibiotic

Answer 40

susceptible

Question 41

The ____ technique can be used to test effectiveness of disinfectants and antiseptics as well as antibiotics

Answer 41

Disk diffusion