Lab Practical Exam 2 Flashcards
3 categories of tests that can be performed on a urine sample
- Chemical analysis
- Direct observation (gross inspection)
- Microscopic examination of urine sediments
4 categories of urine sediments
- Casts
- Cells
- Crystals
- Mucus threads
Abnormal urinalysis results & possible causes: glucose present
- Possible dietary cause
- Possible disease cause
- Large meal consumed
- Uncontrolled diabetes mellitus
Abnormal urinalysis results & possible causes: high pH (> 8.0)
- Possible dietary cause
- Possible disease cause
- Diet rich in vegetables, dairy products
- Severe anemia
Abnormal urinalysis results & possible causes: high specific gravity (> 1.025)
- Possible dietary cause
- Possible disease cause
- Decreased fluid intake, loss of fluids
- Uncontrolled diabetes mellitus, severe anemia
Abnormal urinalysis results & possible causes: low pH (< 4.5)
- Possible dietary cause
- Possible disease cause
- High protein diet, cranberry juice
- Uncontrolled diabetes mellitus
Abnormal urinalysis results & possible causes: low specific gravity (< 1.010)
- Possible dietary cause
- Possible disease cause
- Increased fluid intake
- Severe renal damage
Abnormal urinalysis results & possible causes: protein present
- Possible dietary cause
- Possible disease cause
- High protein diet
- Severe anemia
Abnormal urine color & possible causes: clear to light yellow color
- Diet
- Drugs
- Disease
- Alcohol
- Phosphate, carbonate
- Uncontrolled diabetes mellitus
Abnormal urine color & possible causes: yellow orange to dark green color
- Diet
- Drugs
- Disease
- Carrots
- Antibiotics
- Bilirubin from obstructive jaundice
Abnormal urine color & possible causes: brown-black
- Diet
- Drugs
- Disease
- Rhubarb
- Antidepressants
- Melanin pigment from melanoma
Abnormal urine color & possible causes: brown
- Diet
- Drugs
- Disease
- Rhubarb
- Barbiturates
- Anemia or liver infections
Abnormal urine color & possible causes: dark wine
- Diet
- Drugs
- Disease
- Beets
- Anti-inflammatory drugs
- Hemolytic jaundice
Abnormal urine color & possible causes: green
- Diet
- Drugs
- Disease
- Green food dye
- Diuretics
- Bacterial infection
Abnormal urine color & possible causes: red to red brown
- Diet
- Drugs
- Disease
- Beets
- Laxatives
- Hemoglobine & urine
Abnormal urine color & possible causes: smoky red
- Diet
- Drugs
- Disease
- Beets
- Anticonvulsants
- Unhemolyzed RBCs from urinary tract
Amylase
- What is it
- What produces it
- What does it do
- Digestive enzyme
- Produced by salivary glands and the pancreas
- Breaks dietary starches down into more simple sugars
Amylase is secreted by the salivary glands and the pancreas.
It breaks down ____
Starch
Bile
- Where is it produced
- Where is it stored
- What is it composed of
- Produced by the liver
- Stored and concentrated in the gallbladder
- Composed of bile pigments, bile salts, and cholesterol
Bile salts
- Function
- Emulsifier
Biuret reagent
- What is it
- How are results exhibited
- Protein indicator
- Detects the presence of peptide bonds
- pale blue = negative for protein
- pale purple = positive for protein
- pink = shorter peptides present
Bromothymol blue
- What does it indicate
- How are results exhibited
- Chemical pH indicator
- Results
- blue = pH > 7
- yellow = pH < 7
Cholesterol
- What is it
- Molecular structure
- Lipid
- Over 20 carbon atoms configured into 4 rings
Fatty acids
- Molecular structure
- Polar or nonpolar?
- Long chains of carbon with hydrogen attached
- Nonpolar
Lipid
- What is it
- Molecular structure
- Macromolecule
- Hundreds of molecules of mostly carbon and hydrogen
Phospholipid
- What is it
- Molecular structure
- Lipid
- Structure
- 3-carbon glycerol molecule
- Phosphate group; and
- 2 fatty acids
What is an emulsifier
A substance that has a polar and nonpolar component that can disperse fat into smaller particles in water or other polar solutions.
Which kidney is higher, the left or the right?
Left kidney is slightly superior than the right kidney
Expiratory reserve volume (ERV)
Air in excess of tidal volume that can be exhaled with maximum effort
(1,200 mL)
Forced expiratory volume in 1 second (FEV1) % formula
- FEV1 / FVC * 100 = %
- Most important measurement
Function of the respiratory system
Exchange gases with the environment in a process known as ventilation (breathing)
Function residual capacity (FRC)
Volume remaining in the lungs after a normal, passive exhalation
Identify blue structure
Spleen
Identify
Urinary bladder
Identify
Myometrium of uterus
Layer of smooth muscle tissue deep to the endometrium
Identify
Primary follicle of ovary
Identify
Epithelium of esophagus
Verify: Stratified squamous epithelium
Identify
Esophagus
Identify
Alveolus of lung
(high magnification)
Identify
Central v. of liver
Identify
Ureters
Identify
Urethra
Identify
Vas deferens
Identify
Submucosa of duodenum
Identify
Left lung
Identify
Ascending colon
Identify
Uterus
Identify
Spermatic cord
Identify
Epithelium of trachea
(ciliated pseudostratified columnar)
Identify
Mesentery
Identify
Trachea
Identify
Urinary bladder
Identify
Leydig cells of testis
Identify
Right lung
Identify
Duodenum of the small intestine
Identify
Adventitia of esophagus
Identify
Flow transducer
Identify respiratory & digestive systems
Identify
Submucosal gland of duodenum
(Brunner’s gland)
Identify
Gallbladder
Identify
BIOPAC
Identify
Testis
Identify
Sigmoid colon
Identify
Secondary follicle in ovary
Identify
Pancreas
Identify
Ovarian follicle of ovary
Identify
Ovaries
Identify
Epididymis
Identify
Epithelium of endometrium of uterus
Layer closest to the lumen and containing many glands with tube-like structures
Identify
Urinary bladder
Identify
Oocyte of ovary
Identify
Endometrium of uterus
Identify
Urinary bladder
Identify
Villus of duodenum
Identify
Urethra
Identify
Alveolar duct of lung
Identify
Of duodenum
Identify
Muscularis mucosae of esophagus
Identify
Esophagus
Identify
Identify
Interstinal gland of duodenum
Identify
Cardiac notch
Identify
Mucosa of esophagus
Identify
Spleen
Identify
Pancreas
Identify
Epididymis
Identify
Submucosa of esophagus
Identify
Spermatozoa (pleural) of testis
Spermatozoon (singular) of testis
Identify
Jejunum of small intestine
Identify
Identify
Primary oocyte
Identify
Urinary bladder
Identify
Transverse colon
Identify
VERIFY: ref card 76
Mucosa of duodenum
Identify
Alveolar sac of lung
Identify
Ureters
Identify
Electronic water bath
Identify
Spleen
Identify
Urinary bladder
Identify
Appendix
Identify
Epididymis
Identify
Vagina
Identify
Muscularis externa of esophagus
Identify
Primordial follicle of ovary
Identify
Renal tubules with simple cuboidal epithelium
(Kidneys)
Identify
Vas deferens
Identify
Lumen of testis
Identify
Pancreatic islets
Identify
Glomerulus of renal corpuscle (kidney)
Identify
Urethra
Identify
Descending colon
Identify
Bowman’s capsule of renal corpuscle (kidney)
Identify
Scrotum
Identify
Stomach
Identify
Alveolus of lung
(low magnification)
Identify
Ileum of the small intestine
Identify
Muscularis externa of duodenum
Identify
Hepatocytes in liver
(In the hepatic cords)
Identify
Hepatic sinusoid in liver
(Sinusoids are spaces between hepatic cords)
Identify
Seminiferous tubule of testis
Identify
Spermatic cord
Identify #1
Lungs
Identify #10
Ascending colon
Identify #11
Transverse colon
Identify #12
Descending colon
Identify #13
Sigmoid colon
Identify #14
Spleen
Identify #15
Mesentery
Identify #16
Pancreas
Identify #17
Appendix
(vermiform)
Identify #18
Caecum
Identify #19
Esophagus
Identify #2
Trachea
Identify #2
Trachea
Identify #20
Kidneys
Identify #21
Ureters
Identify #22
Urinary bladder
Identify #22
Urinary bladder
Identify #22
Urinary bladder
Identify #23
Urethra
Identify #23
Urethra
Identify #24
Scrotal sac
(scrotum)
Identify #25
Testis
Identify #26
Vas deferens
(ductus deferens)
Identify #27
Penis
Identify #28
Epididymis
Identify #29
Spermatic cord
Identify #3
Diaphragm
Identify #30
Vagina
Identify #31
Uterus
Identify #31
Uterus
Identify #32
Ovary
Identify #32
Ovaries
Identify #4
Larynx
Identify #4a
Epiglottis
Identify #5
Greater omentum
(part of the peritoneum)
Identify #6
Liver
Identify #7
Gallbladder
Identify #8
Stomach
Identify #8
Stomach
Identify #9
Small intestine
(comprises the duodenum, jejunum, ileum)
Identify (arrow)
Hyaline cartilage of trachea
Identify open space at top (arrow)
Lumen of trachea
Identify
Terminal bronchiole of lung
(low magnification)
Identify
Conducting bronchiole of lung
(low magnification)
Identify - female pelvis
Identify - female pelvis
Identify - male pelvis
Identify - male pelvis midsagittal
Identify female reproductive system
Identify form & function
Acini (exocrine pancreas)
Secrete digestive enzymes
Identify male reproductive & urinary systems
Identify on spirogram
Identify outmost layer at the bottom
Serosa of duodenum
Identify respiratory & digestive systems (2)
Identify the different urine sediment categories
Identify the two parts
Head and flagellum
Identify white space at the top
Lumen of duodenum
Identify white space to the left
Lumen of esophagus
Inspiratory capacity (IC)
- What is it
- How is it calculated
- Maximum volume of air that can be inspired after reaching the end of a normal, quiet expiration
- Sum of the TIDAL VOLUME and the INSPIRATORY RESERVE VOLUME
Inspiratory reserve volume (IRV)
Air in excess of tidal volume that can be inhaled with maximum effort
(3,000 mL)
Lugo’s solution
- What is it
- What does it detect
- How are results exhibited
- Reagent
- Detects the change in starch level
- pale yellow = absence of starch
- purple / black = positive for starch. Progressively lighter as less starch is present
Microscopic view of oil/water mixture. Which side has an emulsifier?
Right
Name the four colons of the large intestine, in order
- Ascending colon
- Transverse colon
- Descending colon
- Sigmoid colon
Name the organ where urine is stored
Urinary bladder
Nonpolar
- Covalent bond that produces a molecule without any charge
- Common when many of the same or similar atoms are bonded
Normal urine characteristics
- Color
- Turbidity
- pH range
- Specific gravity
- Sediments
- Solutes (~5%)
- Color: light yellow to dark golden yellow
- Turbidity: clear
- pH range: 4.5 - 8
- Specific gravity: 1.010 - 1.025
- Sediments: epithelial cells, hyaline casts, crystals, mucous threads, bacteria
- Solutes: electrolytes, urea, uric acid, creatinine, hormones, very small amounts of protein, glucose, bilirubin, ketones
Indications of obstructive airway diseases
- FEV1 / FVC % = < 75% (normal is 80%)
- FVC = > 80% of predicted value
- RV increased
List 4 obstructive airway diseases
- Asthma
- Bronchitis
- COPD (chronic bronchitis & emphysema)
- Emphysema
Pancreatic lipase
- Where is it produced
- What does it do and where
- Produced by the pancreatic acinar cells
- Chemically digests triglycerides (substrate) into fatty acids, glycerol, and some monoglycerides (products) within the small intestine
Pepsin
- Function
- What produces it
- Environment it is most active
- Digestive enzyme
- Breaks larger proteins into shorter chain peptides
- begins in the stomach
- produced by the chief cells of the stomach
- Activity is greatest in an acidic environment, such as the stomach, which secretes hydrochloric acid (HCl)
List the 2 phases of ventilation (breathing)
- Inhalation
- Exhalation
Place the following in order from inferior to superior:
cecum, descending colon, stomach
- Cecum
- Descending colon
- Stomach
Polar
- Covalent or ionic bond that produces a molecule with electrical charges
- Common when atoms that are very dissimilar are bonded
Residual volume (RV)
Volume of air remaining in the lungs after maximum forceful expiration
List 4 restrictive lung expansion diseases
- Acute respiratory distress syndrome
- Asbestosis
- Infant respiratory distress syndrome (lack of pulmonary surfactant)
- Pulmonary fibrosis
Indications of restrictive lung expansion diseases
- FEV1 / FVC% = > 75% (80% close to normal)
- FVC = < 80% of predicted value
- TLC decreased
Restrictive lung diseases
- Affect on physiology
- Affect the FVC and FEV1 amounts proportionally (both values will drop)
- FEV1 / FVC % remains close to normal
Saturated fatty acids
- Molecular structure
- Single carbon-to-carbon bonds with hydrogen atoms attached
Specific gravity
- What is it
- What does it indicate
- Density of a fluid as compared to distilled water
- Indicator for osmolarity of the fluid
- Note: specific gravity is a comparative number and therefore has no unit associated with it
Spirometer function
Measures lung volume
Structures of the female reproductive system
- Fallopian tube
- Ovary
- Uterus
- Vagina
Structures of the male reproductive system
- Epididymis
- Penis
- Prostate gland
- Seminal vesicle
- Testis
- Urinary bladder
- Urethra
- Vas deferens
Structures of the respiratory system
- Diaphragm
- Larynx
- Left lung
- Right lung
- Trachea
Structures of the urinary system
- 2 Kidneys
- Urethra
- Urinary bladder
- 2 Ureters
T/F
Fatty acids may be saturated or unsaturated
True
T/F
Lipids in liquid form are oils that do not mix with water because water is polar
True
T/F
Normal urine contains small numbers of body cells
True
T/F
Saturated fatty acids have all single carbon-to-carbon bonds
True
T/F
Unsaturated fatty acids contain one (monounsaturated) or more (polyunsaturated) double bonds between carbons
True
Tidal volume
Volume of air inhaled and exhaled in one cycle during quiet breathing
(500 mL)
Total lung capacity (TLC)
Maximum amount lungs can hold
(4650-6400 mL)
Total lung capacity (TLC) formula
TLC = IRV + TV + ERV
Triglycerides
- Molecular structure
- How is lipid catabolism visualized
- 1 glycerol molecule and 3 fatty acid chains
- Can visualize how much lipid catabolism occurs by using a chemical pH indicator
- The more the fatty acids accumulate, the more acidic the solution becomes
Turbidity
- Describe
- What does it indicate
- Visual quality based on clarity (cloudiness) of solution
- Abnormal urine contents such as protein, salts, cells and cellular contents
Unsaturated fatty acids
- Molecular structure
- Contains one or more double bonds between carbons with hydrogen atoms attached
Urine sediment casts
- Describe
- Casts & their indications
- normal
- RBC
- WBC
- waxy
- Cylindrical masses of cells or other substances that collect in the distal convoluted tubule or the collecting ducts and are eventually flushed out by the flow of urine
- Indications
- normal urine contains hyaline casts
- RBC casts indicate glomerular damage
- WBC casts indicate infection
- waxy casts are the result of WBC casts that are retained in the tubules
List cells in urine sediment
- Epithelial cells (renal tubule cells, transitional cells, squamous cells)
- Erythrocytes
- Leukocytes
Urine sediment mucus threads
- Describe
- Describe normal & abnormal indications
- Long, thin strands of mucus
- Normal urine contains small quantities of mucus threads
- Large quantities indicate infection, especially STDs
Urine sediments
- Describe
- What does it include?
- Microscopic solids that collects at the bottom of a centrifuged urine sample
- Includes normal and abnormal urine components
Describe the ventilation process
- Air travels through the structures of the respiratory system to the small air sacs (alveoli) where the gases O2 and CO2 can be exchanged with the blood
Vital capacity (VC)
Maximum quantity of air in one breath
(4500-5500 mL)
Fatty acids
- Molecular structure
- Polar or nonpolar?
- Long chains of carbon with hydrogen attached
- Nonpolar
What do high numbers of erythrocytes (RBC) casts indicate?
- Menstruation
- Glomerular damage
- Trauma to the urinary tract
What do high numbers of leukocytes (WBC) casts indicate?
Infection
What do high numbers of renal tubule cells indicate?
Kidney disease
What four structures are in the spermatic cord?
- Epididymis
- Pampiniform plexus
- Testicular artery and vein
- Vas deferens
What instrument is used to measure specific gravity?
Hydrometer
What is a hydrometer that is specifically calibrated for measuring urine called?
- Urine hydrometer; or
- Urinometer
What is a reagent
Chemical indicator that detects the presence of fatty acids, proteins, and starch
What is carbon dioxide
- A waste product of metabolism
- Must be removed from the body by exhalation
What is the last region of the small intestine called?
Ileum
What is the most common lipid?
Triglyceride
What is this?
Hydrometer
Urine hydrometer if measuring specific gravity of urine
What must chemical digestive reactions have?
- Substrate: the substance that the enzyme is acting on (it undergoes a chemical change)
- Enzyme: the protein catalyzing this reaction (it can often be reused and/or recycled)
What organ may be mistaken for a kidney due to its color and proximity?
Spleen
What organs create urine?
- Bladder
- Kidneys
- Ureters
- Urethra
What part of the respiratory system prevents choking on food/liquids?
Epiglottis
To create a negative control for an enzymatic reaction, what would not be included?
Substrate, enzyme, or both
Which part of the peritoneum extends inferiorly below the stomach to cover all the rest of the abdominopelvic organs?
Visceral peritoneum
Which part of the peritoneum holds the loops of the small intestine together?
Mesentery
Why do tissues need oxygen for internal respiration?
To make ATP