Lab Practical Flashcards
Convert 1km to 1mm. What are their powers? Convert 1mm to 1nm
km = 10^3 m = 10 mm= 10-^3 um = 10-^6 nm = 10-^9
1km = 1,000,000mm 1mm= 100nm
What is the relationship of water volume to water mass?
1 mL = 1 gram
What does Benedict’s reagent detect? How? Colors?
Glucose, sugars. Must be heated up. Blue -> Green, yellow, brick, black
What does Iodine detect? How?
Starch, iodine slips into the amylose coil of starch and produces color change (amber -> blue, black)
What does Biuret detect? How? Colors?
Detects peptide bonds, a copper ion forms violet-colored coordination complexes
What’s the difference between osmosis and diffusion?
- diffusion = the net movement of molecules across a membrane
- osmosis = the net movement of H2O molecules across a membrane
Know the symbols for metric units
G, M, K, 10^0, m, u, n
gram, meter, kilo, mili, micro, nano
How do you read a meniscus?
from the bottom line
check increment marks (x1, x2, x5, etc)
What is the total magnification of the low, medium, and high power lens objectives?
40x, 100x, 400x
Know key parts of the microscope
eyepiece, coarse adjustment, fine adjustment, stage, lamp, stage adjustment, light intensity
What do Eukaryotes have that Prokaryotes do not? What is their difference in size?
Mitochondria, nucleus, nucleolus, cell membrane.
Proks are 1-10 um, Euks are 10-100 um
What’s the difference between qualitative and quantitative test results?
Qualitative = quality, characteristics, etc.
Quantitative = numbers; how much data
What’s the difference between osmosis and diffusion?
- diffusion = the net movement of molecules across a membrane
- osmosis = the net movement of H2O molecules across a membrane
What’s the difference between a hypotonic, hypertonic, and isotonic solution?
- hypotonic solutions have more water (or solvent) to solute (like salt) in contrast to another solution—when compared to another solution
- hypertonic solutions have more solute (salt) than water (solvent)—when compared to another solution
- isotonic solutions are equal—when compared to another solution
What are the solutions used in potato juice?
catechol & catechol oxidase