Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbio > Lab Practical 2 > Flashcards

Lab Practical 2 Flashcards

1
Q

Simple stain is for seeing

A

Morphology

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Gram stain is

A

To differentiate gram negative and gram positive bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Gram positive bacteria is

A

Streptococcus
Staphylococcus
Listeria
Corynebacterium
Bacillus
Clostridium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Gram Negative Bacteria is

A

Escherichia
Hemophilus
Neisseria
Salmonella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is the cell wall structure of Gram-Positive bacteria

A

2 Layers
Thick Peptidoglycan
Plasma Membrane

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is the cell wall structure of gram-negative bacteria

A

3 Layers
Outer Membrane
Thin peptidoglycan
Plasma Membrane

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Bacillus Anthracis is

A

Gram Positive
Rod Morphology
Arrangement: Streptobacillus

Causes anthrax

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Bacillus Cereus is

A

Gram Positive
Rod Morphology
Arrangement: Streptobacillus
Causes Food poisoning (associated with undercooked rice)
Has Endospores

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Bordetella Pertussis is

A

Gram negative
Morphology: Rods, coccobacillus
Arrangement: Single or in pairs
Causes Pertussis or whooping cough

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Borrelia Burgdoferi is

A

Gram negative
Morphology: Spirochete
Arrangement: Grouping
Causes Lyme Disease

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Clostridium Botulinum is

A

Gram positive
Morphology: Rods
Arrangement: Grouping
Causes Botulism (paralysis), has endospores

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Clostridium difficile is

A

Gram positive
Morphology: Rods
Arrangement: Grouping
Normal flora that is nosocomial (hospital borne)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Clostridium perfirgens is

A

Gram Positive
Morphology: rods
Arrangement: grouping
Causes gas gangrene, food poisoning

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Clostridum tetani is

A

Gram Positive
Morphology: Rods
Arrangement: Grouping
Causes tetanus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Corynebacterium diphtheriae is

A

Gram Positive
Morphology: Rods
Arrangement: V shape
Causes Diphtheria (an acute, highly contagious bacterial disease causing inflammation of the mucous membranes, formation of a false membrane in the throat that hinders breathing and swallowing, and potentially fatal heart and nerve damage by a bacterial toxin in the blood. It is now rare in developed countries because of immunization.)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Escherichia Coli is

A

Gram negative
Rod Morphology
Arrangement: Grouping
Causes Food poisoning (is food handled comes from poor hygiene after using bathroom)
O157:H7 is the deadly strain.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Hemophilus influenzae is

A

Gram Negative
Rod morphology
Causes Pinkeye, upper respiratory infections

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Klebsiella pneumoniae is

A

Gram Negative
Rod Morphology
Causes pneumonia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Lactobacillus acidophilus is

A

Gram Positive
Rod morphology
Normal vaginal flora, maintains acidic vaginal pH

20
Q

Mycobacterium leprae is

A

Acid-Fast (red)
Morphology - Rods
Disease: Leprosy (Hansen’s disease)

21
Q

Mycobacterium tuberculosis is

A

Acid-Fast (red)
Morphology- Rods
Disease - Tuberculosis

22
Q

Neisseria gonorrhoeae is

A

Gram negative
Morphology - Coccus
Arrangement - Diplococci
Causes gonorrhea

23
Q

Neisseria meningitidis is

A

Gram Negative
Morphology - Cocci
Arrangement - Diplococci
Causes meningitis

24
Q

Pseudomonas aeruginosa is

A

Gram negative
Morphology - Rods
Nosocomial opportunistic

25
Q

Salmonella typhi is

A

Gram Negative
Morphology - Rods
Causes Typhoid fever, spread through contaminated food or water

25
Q

Staphylococcus aureus is

A

Gram positive
Morphology - Coccus
Arrangement - Staphylococcus
Nosocomial opportunistic, lives in the nose

25
Q

Streptococcus pyogenes is

A

Gram positive
Morphology - coccus
Grouping - Streptococcus
Causes strep throat
Opportunistic, nosocomial

26
Q

Streptococcus pneumoniae is

A

Gram Positive
Morphology - Coccus
Arrangement - Diplococci
Causes pneumonia, meningitis

27
Q

Treponema pallidum is

A

Gram negative
Morphology - Spirochete
Causes Syphilis
can be transferred via head of sperm (observable by examining a sperm infected by Treponema pallidum)

28
Q

Organic compounds comprised of carbon and hydrogen atoms are called

A

hydrocarbons

29
Q

The use of microbes to cleanup pollutants is called

A

Bioremediation

30
Q

What does ELISA stand for?

A

Enzyme linked immunosorbent Assay

31
Q

What type of ELISA test did we do in class?

A

Indirect ELISA

32
Q

What disease did our simulated ELISA test, test for?

A

HIV

33
Q

What tool did we use in ELISA lab to collect the specimens?

A

microtiter plate

34
Q

What does the ELISA test look for?

A

The presence/absence of antibodies against a particular pathogen

35
Q

What transfers the antibodies to the microtiter plate?

A

Pipets

36
Q

What letter of the alphabet is an antibody shaped like?

A

Y

37
Q

An antigen binds to what part of the antibody?

A

The ends of the antibody

38
Q

What organisms were used for bioremediation lab?

A

Pseudomonas and Penicillium

39
Q

What is the primary stain and the counterstain for endospores?

A

Malachite Green - Primary
Safarin - Counterstain

40
Q

What is the primary stain and the counterstain for acid-fast?

A

Carbol Fuschin - Primary (red)
Methylene blue - Counterstain (blue)

41
Q

What were the two organisms used in the acid-fast staining? which one retained the acid-fast stain?

A

Staphylococcus epidermidis
Mycobacterium non-chromogenium (retained the stain)

42
Q

What were the two organisms used in the endospore staining? Which one retained the endospore stain?

A

Bacillus cereus (retained the endospore stain)
Escherichia coli

43
Q

What shape is spiral without movement?

t

A

Spirillum

44
Q

What shape is spiral with movement?

A

Spirochete

Microbio (5 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions