Lab Practical 2

Question 1

Type of plate that Grows ALL (2):

Answer 1

nutrient agar; blood agar

Question 2

What does nutrient agar contain?

Answer 2

peptone, nutrients, beef broth

Question 3

What does blood agar contain?

Answer 3

5% sheep’s blood and TSA (trypticase soy agar)

Question 4

Blood agar differentiates for _____.

Answer 4

hemolysis

Question 5

MAC stands for?

Answer 5

MacConkey

Question 6

What is the factor in MAC?

Answer 6

1.5% bile salts and crystal violet

Question 7

What does MAC grow? What does it inhibit?

Answer 7

Grow: Gram - Inhibit: Gram +

Question 8

What does MAC differentiate?

Answer 8

lactose fermenters

Question 9

Which media produces pink/purple colonies when fermenting lactose and white when not?

Answer 9

MAC

Question 10

This media differentiates by hemolysis and grows staphs and streps:

Answer 10

PEAB

Question 11

PEAB stands for:

Answer 11

phenol ethyl alcohol blood

Question 12

What kind of media inhibits gram negative?

Answer 12

PEAB

Question 13

What kind of media grows Staphylococcus?

Answer 13

MSA

Question 14

What type of media differentiates E.coli?

Answer 14

EMB - eosin and methylene blue

Question 15

What portion of thioglycolate shows green in the presence of oxygen?

Answer 15

bromthymol blue

Question 16

What has phenol red as a factor?

Answer 16

MSA

Question 17

What contains 7.5% bile salt?

Answer 17

MSA