Final Exam Flashcards
Who established the system of scientific nomenclature?
C. Linnaeus
Term for ‘bacteria’: means, pre-nucleus and single celled:
prokaryote
This type of bacteria is single celled, does not have a membrane bound nucleus, has a peptidoglycan cell wall, divides by binary fission, and derives nutrition from organic or inorganic chemicals or photosynthesis:
prokaryote
This type of bacteria is a prokaryote that exists in environments that don’t inhabit and is not pathogenic. It also lacks peptidoglycan cell walls:
archaea
What is the eukaryote that has a membrane bound nucleus, chitin cell wall, produces spores and absorb organic chemicals for energy?
fungi
Unicellular fungi:
yeast
multicellular fungi:
molds and mushrooms
Unicellular eukaryotes that absorb or ingest organic chemicals from their surrounding. May be motile via pseudopods, cilia, or flagella. Free-living or parasitic and can perform phagocytosis.
protozoa
Eukaryote with cellulose cell walls. Use photosynthesis for energy. Produce oxygen and carbohydrates. Can live in soil, water, salt water:
algae
Acellular with a DNA or RNA core surrounded by a protein coat and may be enclosed in a lipid envelope. Inert outside living hosts and only replicate when are in a living host cell:
viruses
Term for parasitic flatworms and roundworms:
helminths
Eukaryote that is multicellular, not strictly microscopic, and live off of human tissue:
Multicellular animal parasites
The Tree of Life consists of which domains of cellular organization?
Bacteria, Archaea, Eukarya
What is the broadest form used to classify microorgnaisms?
Three domains categorized as the Tree of Life
Eukarya consists of:
protists, fungi, plants, animals
Who was responsible for discovering pasteurization and fermentation?
Pasteur
This person’s discoveries showed the relationship between microbes and disease, immunity, and antimicrobial drugs (antibiotics):
Pasteur
Who developed the first vaccine (smallpox)?
Jenner
Who discovered the first antibiotic (penicillium)?
Fleming
Science of classifying organisms that shows degree of similarity among organisms
taxonomy
Study of the evolutionary history of organisms
Systematics or phylogeny
Group of closely related organisms that breed among themselves (capable of sexual reproduction):
eukaryotic species
Order of the Taxonomic Hierarchy from greatest to least:
domain, kingdom, phylum, class, order, family, genus, species
Kingdoms for eukaryotes:
protista, fungi, pantae, animalia
rod-shaped
bacillus
spherical
coccus
spiral shapes of bacteria:
vibrio, spirillum, spirochete
cell shapes specific to archaea:
star-shaped; rectangular
pairs of cells
diplo
clusters
staphylo
chains
strepto
groups of four
tetrads
cubelike structure eight cells:
sarcinae
What makes up a prokaryote’s outermost layer?
glycocalyx
What structure’s main job is locomotion?
flagella
Term for hairlike appendages that allow for attachment? Many GI tract microbes have these so they can attach to the inner wall of the intestine without getting washed away.
fimbrae
What prevents a prokaryotic cell from lysis during osmotic changes?
peptidoglycan cell wall
Movement of a solute from an area of high concentration to an area of low concentration; continues until molecules reach equilibrium:
simple diffusion
Solute combines with a transporter protein or carrier molecule in the membrane so that ions and larger molecules are transported across a membrane WITH the concentration gradient:
facilitated diffusion
The movement of water across a selectively permeable membrane from an area of high water an area of lower water concentration:
osmosis
What breaks down complex molecules providing energy and building blocks for anabolism?
catabolism
What is an endergonic reaction?
anabolism
What uses energy and building blocks to build complex molecules?
anabolism
Catabolism releases energy by ___ of molecules.
oxidation
gain of electrons
reduction
oxidation reaction paired with a reduction reaction:
redox reaction
What is used as electron carriers in redox reactions?
NAD+ and FAD
Name the physical requirements for microbial growth:
temperature, pH, osmotic pressure (balance between solutes and water)
Name the chemical requirements for microbial growth:
water, carbon, N, S, P, O, trace elements, organic growth factors
cold loving microbes:
psychrophiles
moderate-temperature-loving microbes
mesophiles
These microbes are found in hot springs and their optimum growth temp. is 50-60 C
thermophiles
These microbes have an optimum growth temp greater than 80 C
hyperthermophiles
Obligate aerobes _____oxygen and run the electron transport chain.
require
Can grow with (tolerates) or without O2 via fermentation or anaerobic respiration (E. coli):
facultative anaerobes
These microbes can’t tolerate O2 and are harmed by it:
obligate anaerobes
What type of media is used to suppress unwanted microbes and encourage desired microbes?
selective media
What type of media allow distinguishing of colonies of different microbes on the same plate?
differential media
Phases of bacterial growth:
lag phase, log phase, stationary phase, death phase
During which stage of phase growth is there intense activity preparing for growth, but no division?
lag phase
During which stage of phase growth is there exponential increase in population?
log phase
During which stage of phase growth is there a period of equilibrium?
stationary phase
During which phase of growth is population decreasing at a logarithmic rate?
death phase
Term that refers to bacterial contamination:
sepsis
Term that refers to the absence of significant contamination:
asepsis
Term for removing and destroying all microbial life:
sterilization
This is used to destroy harmful microorganisms from fomites:
disinfection
Term for destroying harmful microorganisms from living tissue:
antisepsis
What is degerming?
mechanical removal (rather than killing) of microbes from a limited area (ex: handwashing).
This is used to lower microbial counts on eating utensils to safe levels:
sanitization
Term for the lowest temperature at which all cells in a liquid culture are killed at 10 minutes:
Thermal death point (TDP)
Term for the minimal time for all bacteria in a liquid culture to be killed at a particular temperature:
Thermal death time (TDT)
Which guidelines indicate the severity of treatment required to kill a given population of bacteria?
Thermal death point (TDP) and Thermal death time (TDT)
What is Decimal reduction time (DRT)?
minutes to kill 90% of a population at a given temperature
Name three types of radiation:
ionizing radiation, nonionizing radiation, microwaves
How does ionizing radiation work?
ionizes water to create reactive hydroxyl radicals; damages DNA by causing lethal mutations which can cause a cell to die.
How does nonionizing radiation work?
does NOT cause water to split into ions; damages DNA by creating thymine dimers (how melanoma forms)
How many phyla does the domain bacteria have?
14
List the gram-negative phyla:
proteobacteria, cyanobacteria, chlamydiae, bacteriodetes, fusobacteria, spirochaetes, deinococcus
List the gram-positive phyla:
firmicutes, actinobacteria
What is the largest phyla of bacteria?
Phylum Proteobacteria
Which phylum carry out oxygenic photosynthesis, nitrogen fixation, thought to play important role in increasing O2 concentration in the atmosphere
Phylum Cyanobacteria
What phylum causes the most common STD in the U.S.?
Phylum Chlamydiae
Are most fungi pathogenic?
no. There are greater than 100,000 species and only about 200 are pathogenic
What are the two growth patterns of fungi?
unicellular yeasts; multicellular molds and mushrooms
Name the five types of mycoses:
systemic mycoses, subcutaneous mycoses, cutaneous mycoses, superficial mycoses, opportunistic mycoses
Name four Phyla of medically important fungi:
zygomycota, microsporidia, ascomycota, basidiomycota
Do algae cause infectious diseases?
Algae are not pathogenic but some produce toxins which could affect humans and produce neurological problems.
Name the three medically important classes of Protozoa:
Sarcomastigophora, Ciliophora, Apicomplexa
What class of protozoa includes amoeba and flagellated protozoans?
Class Sarcomastigophora
Give examples of Class Sarcomastigophora:
Trypanosoma, Trichomonas, Leishmania
Give an example of a pathogen in Class Ciliophora:
Balantdidium coli (causes colitis and is the only parasitic ciliate of humans)
Give an example of Class Apicomplexa:
Plasmodium species, Toxoplasma gondi
Term for the primary host who harbors the sexually reproducing parasite:
definitive host
Term for the secondary host that harbors the larval stage of a parasite:
intermediate host
How do arthropods act as vectors in causing disease?
They transmit disease on their feet or in their body without being infected themselves (mechanical vector); or when an infected arthropod transmits a pathogen by a bite or other wound (biological vector)
What are the minimum components of a virus?
nucleic acid, protein coat
Name the four possible components of a virus:
nucleic acid, capsid, envelope, spikes
If a virus has an envelope, what is it made of?
lipid, protein, and carbohydrate coating made from cell components
Name some enveloped viruses that are human pathogens:
Herpes, mumps, measles, rubella, hep B, Corona, rabies, influenza, poxviruses, HIV
What type of virus (naked or enveloped) is easier to destroy with chemical agents?
enveloped viruses
What type of virus (naked or enveloped) is harder for our immune system to destroy?
enveloped viruses