lab practical 1 Flashcards
what shape is E. Coli
bacillus
lyme disease and syphillus shape
spiral
what is the purpose of aseptic techniques
prevents contamination of sample, handler, people around
how to label plates
bottom of plates not the lid
what is smear prep important to see
cell morphology, arrangmenet, internal structures
what color are gram positive cells
purple
what color are gram negative cells
pink
which gram cell has the thickest peptidoglycan
gram positive
primary gram stain
crystal violet, will stain both bacteria purple
gram’s iodine
the mordant/fixing reagant
ethyl alcohol
decolorizer in gram stain procedure, gram negative purple will be washed out
safranin
gram counterstain, will color gram negative bacteria red/pink
what is the cause of bacterial motility
chemotaxis
what is true movement
chemotaxis, movement from point A to point B
what is brownian motion
not true movement, just movement from being under the cover glass
what is the purpose of pure culture techniques
to dilute bacteria concentration for just one colony
what bacteria was used during pure culture
e. coli, s. aureus, p. aeruginosa
what color is e. coli
white
what color is p. aeruginosa
green
what color is s. aureus
gold
what is the purpose of the quadrant streak method
by the 4th streak there should hopefully be individual colonis
what is the purpose of the pour plate technique
serial dilution
what color are endospores
green
what color are the vegetative/germinating cells
pink
what is the acid fast bacteria?
mycobacterium smegmatus
what color is the acid fast bacteria
m. smegmatus should be pink
what color is the non acid fast bacteria?
blue/purple
what is the non acid fast bacteria?
s. aureus
what is present in the acid fast cell wall
mycolic acid
what allows for dye penetration into the cell wall
phenol in fuschin
can you see a bacteriophage without a microscope?
no
how do we see bacteriophages?
using plaque essays
what bacteria is used for plaque essays
e. coli B and T4
confluent lawn is…
bacterial growth
plaques are…
clearing of bacteria in a lawn due to lysis
what bacteria is used during the motility lab
b. cereus, pneumoniae
what shapes of bacteria do motility occur
rod and spiral shaped
what motility techniques did we use
plate and tube stab, and hanging drop slide
endospore stain procedure
malachite green for 10 min
rinse with water 30 seconds
counterstain with safranin 1 min
acid fast stain procedure
fuschin 10 min
wash with water
decolorize with acid alcohol
counterstain with methylene blue
can you use the course focus knob on 40 and 100x?
no
what kind of microscopy are we doing?
brightfield
what materials did we use during the environmental bacteria experiment?
nutrient broth tubes and agar plates
why do we spray down work station before starting lab?
to kill the vegetative cells and viruses
what should you do before heat fixing the smear prep slide
air dry it
what is the protein protective coat around the endospore?
the exosporium
are brownian motion and chemotaxis the same thing?
no
what part of the virus protects the nucleic acid
capsid
plaque forming units are used to…
they can be counted to determine the number of viral particles effecting bacteria
