Lab one Flashcards
what are the 4 types of basic tissue?
epithelium
Connective
muscle
nervous
what are the two functions of the pancreas?
exocrine and endocrine
what does methyl green stain?
specifically stains DNA
stains the nucleus green
what does Pyronin-Y stain?
specifically stains RNA
Stains the RNA in the nucelous, the ribosomes and the RER in the cytoplasm pink
what is the purpose of a Methyl green, Pyronin-Y stain?
to differentiate between structures containing DNA or RNA
what do you use light microscopy for?
looking at thin sections of tissue using objective lenses of different magnifications
what do you use Transmission electron microscopy for?
uses beams of electrons through the surface of thin sections of tissue to visualize structures at a much higher magnification
used to look at the surface of a cell or structure at high magnification with out sectioning it
How does Scanning electron microscopy work?
uses electron beams to create a three-dimensional image
what does Haematoxylin stain?
Haematoxylin is a basic stain that stains acidic structures purple
Haemotoxylin stains the nuclei purple/black and an area around the nucleus with a purple hue due to RNA
what does Eosin stain?
Eosin is an acidic stain that stains acidic structures red/pink
The cytoplasm stains red/pink
Red blood cells stain vivid red with eosin.
what does Aldehyde acid fuschin stain?
Stains elastic tissue purple
what does Alcian green stain?
Stains microvilli green and mucous in goblet cells blue/green
what does Haematoxylin alum (Gills Haematoxylin) stain?
Stains nucleus of epithelial cells blue
what does Biebrichs scarlet stain?
Stains granules in epithelial cells bright red, also red blood cells and most cell cytoplasm stained red, nuclei red/purple
what does Analine blue + picric acid stain?
stains cytoplasm an organge-red, collagen blue
what is covering epithelia classified by?
classified according to the arragement and shape of the cells, as well as the number of layers
what is simple epithelium?
One layer of cells which are all in contact with the basement membrane
what are some examples of simple epithelium?
Simple squamous
Simple cuboidal
simple columnar
Pseudostratified columnar epithelium
what is stratified epithelium?
has more than one layer of cells and only the deepest layer is in contact with the basement membrane.
what are some examples of stratified epithelium?
stratified squamous
stratified cuboidal
stratified columnar
transitional epithelium
what is glandular epithelia?
Epithelial cells can be grouped into glands to allow focused production of a secreted product. Exocrine glands are broadly divided into two groups based on whether the duct system is simple or branched (compound)
what is the role of tight junctions?
prevents luminal contents passing between cells
what is the role of zondular adherens?
provides structural support and reinforcement between cells
what is the role of desmosomes?
binds epithelial cells together as a tight unit/tissue
what is the role of a hemidesmosome?
provide anchoring support for the cell into the basement membrane via its cytoskeleton
what junctions contain actin?
tight junctions, zonula adherens
what junctions contain cytokeratin?
desmosomes and hemidesmosomes
what are some features of microvilli?
found on the apical membrane
function is absorption
Visible by Electron microscopy (brush border visible with light microscopy)
what are some features of epithelial nuceli/nucleolus?
found on the basal membrane
Function is cell activity
Visible under both light and electron microscopy
what are some features of epithelial mitochondria?
found on the apical or basal membrane
Function is energy provision
Visible under electron microscopy
what is phase contast best used for?
visualizing infixed, unstained tissue
what is conventional bright field microscopy best used for?
visualizing stained and fixed tissue
