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ANAT241- Module 2 > Lab one > Flashcards

Lab one Flashcards

1
Q

what are the 4 types of basic tissue?

A

epithelium
Connective
muscle
nervous

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2
Q

what are the two functions of the pancreas?

A

exocrine and endocrine

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3
Q

what does methyl green stain?

A

specifically stains DNA
stains the nucleus green

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4
Q

what does Pyronin-Y stain?

A

specifically stains RNA
Stains the RNA in the nucelous, the ribosomes and the RER in the cytoplasm pink

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5
Q

what is the purpose of a Methyl green, Pyronin-Y stain?

A

to differentiate between structures containing DNA or RNA

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6
Q

what do you use light microscopy for?

A

looking at thin sections of tissue using objective lenses of different magnifications

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7
Q

what do you use Transmission electron microscopy for?

A

uses beams of electrons through the surface of thin sections of tissue to visualize structures at a much higher magnification

used to look at the surface of a cell or structure at high magnification with out sectioning it

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8
Q

How does Scanning electron microscopy work?

A

uses electron beams to create a three-dimensional image

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9
Q

what does Haematoxylin stain?

A

Haematoxylin is a basic stain that stains acidic structures purple

Haemotoxylin stains the nuclei purple/black and an area around the nucleus with a purple hue due to RNA

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10
Q

what does Eosin stain?

A

Eosin is an acidic stain that stains acidic structures red/pink

The cytoplasm stains red/pink
Red blood cells stain vivid red with eosin.

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11
Q

what does Aldehyde acid fuschin stain?

A

Stains elastic tissue purple

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12
Q

what does Alcian green stain?

A

Stains microvilli green and mucous in goblet cells blue/green

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13
Q

what does Haematoxylin alum (Gills Haematoxylin) stain?

A

Stains nucleus of epithelial cells blue

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14
Q

what does Biebrichs scarlet stain?

A

Stains granules in epithelial cells bright red, also red blood cells and most cell cytoplasm stained red, nuclei red/purple

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15
Q

what does Analine blue + picric acid stain?

A

stains cytoplasm an organge-red, collagen blue

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16
Q

what is covering epithelia classified by?

A

classified according to the arragement and shape of the cells, as well as the number of layers

17
Q

what is simple epithelium?

A

One layer of cells which are all in contact with the basement membrane

18
Q

what are some examples of simple epithelium?

A

Simple squamous
Simple cuboidal
simple columnar
Pseudostratified columnar epithelium

19
Q

what is stratified epithelium?

A

has more than one layer of cells and only the deepest layer is in contact with the basement membrane.

20
Q

what are some examples of stratified epithelium?

A

stratified squamous
stratified cuboidal
stratified columnar
transitional epithelium

21
Q

what is glandular epithelia?

A

Epithelial cells can be grouped into glands to allow focused production of a secreted product. Exocrine glands are broadly divided into two groups based on whether the duct system is simple or branched (compound)

22
Q

what is the role of tight junctions?

A

prevents luminal contents passing between cells

23
Q

what is the role of zondular adherens?

A

provides structural support and reinforcement between cells

24
Q

what is the role of desmosomes?

A

binds epithelial cells together as a tight unit/tissue

25
Q

what is the role of a hemidesmosome?

A

provide anchoring support for the cell into the basement membrane via its cytoskeleton

26
Q

what junctions contain actin?

A

tight junctions, zonula adherens

27
Q

what junctions contain cytokeratin?

A

desmosomes and hemidesmosomes

28
Q

what are some features of microvilli?

A

found on the apical membrane
function is absorption
Visible by Electron microscopy (brush border visible with light microscopy)

29
Q

what are some features of epithelial nuceli/nucleolus?

A

found on the basal membrane
Function is cell activity
Visible under both light and electron microscopy

30
Q

what are some features of epithelial mitochondria?

A

found on the apical or basal membrane
Function is energy provision
Visible under electron microscopy

31
Q

what is phase contast best used for?

A

visualizing infixed, unstained tissue

32
Q

what is conventional bright field microscopy best used for?

A

visualizing stained and fixed tissue

ANAT241- Module 2 (9 decks)

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