Lab Midterm Study Guide Flashcards
How is lactose broken down
lactaid
What are the products of lactose breakdown
glucose and galactose
what are the products of ONPG breakdown
glucose and DNP
Why did the tube turn yellow in the lactaid experiment ?
separation from ONPG into glucose and DNP
what is an example of a control variable ?
water
what was the independent variable in lab 1 ?
lactaid
what was the dependent variable in lab 1 ?
color
experimental group and control group, what’s the difference
experimental group receives the treatment, the control group is not changed
Benedict’s Reagent is used to test for _____
monosaccharides/sugars
What color does Benedict’s Reagent turn when positive
Green (low), yellow, orange, red (high)
What color does Benedict’s Reagent turn when negative
Baby Blue
What is a positive control
Substance that contains molecule that is being tested
What is a negative control
Substance that does not contain the molecule being tested
Iodine is used to test for the presence of ____ which is a ___
amylose : starch
Positive result of the Iodine test is
Blue/Black Color
Negative result of the Iodine test is
Pale Yellow Color
Amylose chemically digests ____ in crackers
starch
Sudan IV Dye and Greasy Spot Test for the presence of _____
oils / triglycerides
Sudan IV Test is what color when positive
Red
Sudan IV Test is what when it’s negative
Not fully mixed, particles will float
What are detergents used for ?
Help dissolve oils in water through EMULSIFICATION
If the paper in the Greasy Spot Test dries within minutes it is
Aqueous
If the paper in the Greasy Spot Test is still wet after a few minutes, their are signs of ___
lipids
The Biuret test is used to test for ____
Proteins
The Biuret Test is ___ when it is a negative result
Blue
The Biuret Test is ____ when it is a positive result
Purple/Violet
The Dih Diphenylamine Test binds ____ and _____ to form a molecule
DNA and Diphenylamine
The Dih Diphenylamine test is ___ when positive
blue
The Dih Diphenylamine test is ___ when negative
no change
What does the iris diaphragm do
Limits the amount of light transmitted
Stage Knob
Moves the stage left to righty
Coarse Focus Knob
Moves the stage up and down
Fine Focus Knob
Focuses the stage quality
What is Microscopy
Study of Microbes using a Microscope
Micrometer Size Range
1 micrometer = 1 / 1000 millimeter
Magnification
Amount of enlargement of an image
Resolution
Ability to see detail
Clarity
Crispness of the image
Formula for FOV
FOV low * Mag Low = FOV high * Mag High
FOV Area Formula
A = pi(r)^2
