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Histopathologic and Cytologic Techniques > LAB M2 Ex2-3 > Flashcards

LAB M2 Ex2-3 Flashcards

1
Q

other name for rocking microtome

A

cambridge

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2
Q

rocking microtome inventor

A

Paldwell Trefall 1881

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3
Q

knife profile of rocking microtome

A

plano concave

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4
Q

rocking microtome is used for

A

celloidin embedded blocks

large paraffin emebedded blocks

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5
Q

thickness of rocking microtome tissue

A

10-12 um

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6
Q

other term for rotary microtome

A

minot

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7
Q

knife profile of rotary microtome

A

biconcave

planoconcave

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8
Q

rotary microtome is used for

A

paraffin or resin embedded blocks

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9
Q

most common microtome for routine lab

A

rotary/ minot microtome

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10
Q

thickness of rotary microtome sections

A

3- 5 um

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11
Q

two types of sliding microtome

A

base sledge

standard sliding

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12
Q

knife is stationary and block is manipulated

A

base sledge

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13
Q

block is stationary and knife is manipukated

A

standard sliding

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14
Q

knife of bade sledge

A

plano concave

plane wedge knife

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15
Q

base sledge is used for

A

large blocks (whole brain), hard tossues, whole mounts

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16
Q

knife of standard sliding microtome

A

planoconcave

plane wedge

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17
Q

use of standard sliding microtome

A

celloidin – oblique knife

large refractory paraffin - straight knife

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18
Q

inventor of sliding microtome

A

adams 1789

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19
Q

inventor of freezing microtome

A

Queckett 1848

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20
Q

knife of freezing microtome

A

plane wedge

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21
Q

use of freezing microtome

A

rapid diagnosis, enzyme, lipids, neurological structures

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22
Q

temperature of cryostat

A

-5 to -30 degrees

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23
Q

size very thin sections of ultrathin microtome

A

60-100 nm

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24
Q

size of semi thin sections of uktrathin microscope

A

0.5- 1 um

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25
Q

use of ultrathin microtome

A

electron microscopy

26
Q

advances the block holder towards pr away the knife

A

coarse handwheel

27
Q

advanves the block only towards the knife by microns

A

fine advancement wheel

28
Q

prevengs block holder from coming down towards blade

A

locking mechanisms

29
Q

hokds the blade

A

blade clamp

30
Q

adjusts the knife angle

A

knife tilt

31
Q

guides ribbins away from the blade towards the operator

A

face plate

32
Q

predetermined thickness for sectioning

A

5-10 um

33
Q

msot common type of microtome knife

A

steel knives

34
Q

disposable knives are coated in

A

PTFE polytetrafluoroethylene

35
Q

sharper than diamon knoves but more fragile

A

glass knives

36
Q

sharper than diamon knoves but more fragile

A

glass knives

37
Q

very expensive knife with long usage span

A

diamond knife

38
Q

concave on both sides

A

biconcave knife

39
Q

length of biconcave knife

A

120 mm

40
Q

one flat side, one concave side

A

planoconcave

41
Q

length of planoconcave knife

A

25 mm

42
Q

has deep curring edge and both straight sides

A

plane wedge knife

43
Q

length of plane wedge knife

A

100 mm

44
Q

bevel angle

A

27- 32

45
Q

cutting angle

A

15

46
Q

clearance angle for paraffin embedded tissues

A

2-4

47
Q

clearance angke for frozen sections

A

5-7

48
Q

angle between the cutring edges

A

bevel angle

49
Q

angle at which maximum penetrayion at minimum distortion is achieved

A

cutting angle

50
Q

angle between upper knife and perpendicular line from block

A

rake angle

51
Q

rake angle for sof tissues

A

high rake angle

52
Q

rake angle for hard tissues

A

low rake angle

53
Q

tip of blade scratched the block

A

too wide clearance angle

54
Q

body of blade scrapes the block

A

too small clearance angle

55
Q

angle between bl9ck surface and lower level of knife

A

clearance angle

56
Q

rougher side of belgian yellow for coarse honing

A

black surface

57
Q

smoother side of belgian yellow

A

yellow side

58
Q

honing us repeated how many times

A

20-30

59
Q

applied before stroping

A

vegetable oil

60
Q

stropping is repeated how many times

A

40-120

Histopathologic and Cytologic Techniques (6 decks)

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