LAB - Kinetic Spectrophotometry...

Question 1

this helps us measure enzyme activity in order to quantify them

Answer 1

kinetic assay = measures rate of rxn

Question 2

gamma-Glutanyktransferase

Answer 2

catalyzes transfer of glutamyl group from a glutamyl peptide to sn AA of another peptide

• several organs but increases in serum are usually from hepatobiliary system
• used in diagnosis for liver diseases such as alcoholic cirrhosis and secondary liver tumors
• this may be used to confirm if an increase in ALP originate in the liver

Question 3

endproduct of the reaction y-GT catalyzes which absorbs strongly at 405 nm

Answer 3

5-amino-2-nitrobenzoate

Question 4

KINETIC assays

Answer 4

• enzyme incubated with substrate
• substrate, product, co-factor measured continuously or several points as a function of time
• allow for demonstration of linearity of a rxn
• measured over a short period of tie = little danger of enzyme inactivation
• warning provided if substrate conctn falls below optimum (NOT in fixed time or end-pt rxn)