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Micro020 > lab final (medias) > Flashcards

lab final (medias) Flashcards

1
Q

what is the purpose of a MAC plate?

A

differentiate lactose-fermenting and lactose non-fermenting gram-negative cells

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2
Q

what are the selective agents in a MAC plate and what do they do

A

crystal violet and bile salts

select for gram-negative and enteric bacteria

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3
Q

what are the differential agents in MAC plate and what do they do

A

lactose and neutral red

lactose is fermented which results in acidic pH and neutral red to turn a bright pink-red color

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4
Q

what is the pH indicator in a MAC plate

A

neutral red

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5
Q

what is the purpose of an MSA plate

A

to determine if the bacteria is halophilic (salt loving) and if the bacteria can ferment mannitol

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6
Q

what does halophilic mean

A

able to grow in high salt environment

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7
Q

what is the purpose of blood agar plate

A

identify hemolysis

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8
Q

blood agar plate has __ media

A

enriched

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9
Q

what is hemolysis

A

breakdown of red blood cells

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10
Q

what is the purpose of sabarouds dextrose agar

A

grow fungi

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11
Q

what is the selective agent in MSA plate and what does it select for

A

salt

gram + staphylococcos

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12
Q

what are the differential agents in MSA plate and what do they differentiate

A

mannitol - fermentation of acids

phenol red - ph indicator

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13
Q

what is the purpose of the simmons citrate test

A

identify the microbe’s ability to use citrate as an energy source

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14
Q

which test uses bromothymol blue

A

sodium citrate

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15
Q

what is the purpose of sim test

A

test ability of organism to reduce sulfur, produce indole, and motility

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16
Q

what is the selective ingredient in the indole test

A

kovac’s reagent

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17
Q

what does kovac’s reagent do in indole test test

A

identifies production of indole

reacts with indole to produce a red compound

18
Q

what is the purpose of mrvp test

A

determine which fermentation pathway is used to utilize glucose

19
Q

what are the products of mrvp test

A

mixed acids or alcohol

20
Q

what is the pH indicator in mrvp test and what does it do

A

methyl red

indicates if pH is below 4.4 which indicates glucose fermentation and acid production

21
Q

what is the purpose of mueller hinton agar

A

used in the kirby-bauer method to determine antibiotic resistance

22
Q

which test is used in the kirby bauer method

A

muller hinton

23
Q

what is the purpose of catalase test

A

determine presence of catalase

24
Q

how do you interpret the results of catalase test

A

hydrogen peroxide is added

in a positive reaction, catalase breaks down hydrogen peroxide and causes bubbles

25
Q

what is the broth dilutions test used for

A

determine MIC (minimum inhibitory concentration) and MBC (minimal bactericidal concentration) of an antimicrobial drug

25
Q

what are negative and positive results for mrvp test

A

negative - yellow

positive - red

25
Q

what are negative and positive results for citrate test

A

negative - green

positive - growth or blue

26
Q

in the sim test, what is a positive and negative reaction for sulfur reduction

A

positive - black

negative - no color change

27
Q

what does a positive and negative result on a MAC plate look like

A

positive - bright pink

negative - yellow

28
Q

what does a positive and negative result look like on a MSA plate

A

positive - yellow

negative - pink

29
Q

what do results look like for lactose broth test

A

positive - yellow

negative - pink

30
Q

how do staph aureus and strep aureus react to catalase

A

staph: catalase positive
strep: catalase negative

31
Q

s aureus and s pyogenes produce what type of hemolysis

A

Beta hemolysis

32
Q

what is the selective ingredient in SDA and what does it do

A

antibiotics - grow fungi

33
Q

what is a positive result for motility test

A

moved away from the stab line

34
Q

what is a positive result for indole test

A

pink

35
Q

what is added to the mrvp test to indicate glucose fermentation and alcohol production

A

koh+

36
Q

what is the purpose of lactose broth

A

identify lactose fermentation and acid production

37
Q

what is the ph indicator in lactose broth

A

phenol red

38
Q

what is a positive result of lactose broth

A

yellow and gas bubble in durham tube

39
Q

which test uses a durham tube for gas collection

A

lactose broth

40
Q

s pyogenes is catalase positive or negative

A

catalase negative

Micro020 (6 decks)

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