[LAB] Experiment 1 Qualitative Analysis of Amino Acids and Proteins

Question 1

The _________ and _________ groups comprise the functional group in a class biologically important compounds.

Answer 1

Amino and Carboxylic Acid Group

Question 2

Which are the building blocks of proteins

Answer 2

Amino Acids

Question 3

Polymers composed of amino acids that are linked together by a petided bond (amide bond)

Answer 3

Proteins

Question 4

_____________ of proteins yields the component amino acids.

Answer 4

Complete Hydrolysis

Question 5

Functional groups present in ______ and ________ react with a variety of reagents to form colored products.

Answer 5

Amino Acids
Proteins

Question 6

The structure of proteins are so much more complex compared to those of ___________ and ________.

Answer 6

Carbohydrates and Lipids.

Question 7

The structure of proteins are so much more complex compared to those of carbohydrate and lipids that it can gbe described in four different ways;

Answer 7

Primary Structures
Secondary Structures
Tertiary Structures
Quaternary Structures

Question 8

Different types of intermolecular forces of attraction between the ___________ and __________maintaine these structures.

Answer 8

R groups
Peptide Backbone

Question 9

The biological activity of a protein depends on its ________ and ___________

Answer 9

Secondary Structure
Tertiary Structure

Question 10

Associated with the alteration of these structures which results in changes in physical properties and loff of biological activity.

Answer 10

Protein Denaturation

Question 11

Denaturation may be caused by the action of ______, ____ and chemical reagents such as ________, __________ and ________, detergents and heavy metal ions such as ______ and ________

Answer 11

Heat, Light
Alcohols, Strong Acids and Bases
Mercury(II) [Ion Hg(II)], Lead (II) ion [Pb(II)]

Question 12

Experiment 1 Title

Answer 12

Qualitative Analysis of Amino Acids and Proteins

Question 13

The students need to bring the following:

Answer 13

Unflavored gelatin powder
One Egg

Question 14

Reagents

Answer 14

• L- Glycine
• L-Trytophan
• L-Arginine
• N-acetylcysteine

-3M sodium hydroxide
- 5% cupric sulfate (CuSO4)
- 10% lead (II) ACETATE (pB(ch3coo)2)
- 0.02% 1 naphthol

• Bromine-water
• Hopkins-Cole Reagent
• Methyl Red Indicator
• 1% Acetic Acid

Question 15

Equipments

Answer 15

Top-loading Balance
Hot Plate/Stove

Question 16

Other materials

Answer 16

• Alcohol Lamp
• pH Paper

Question 17

The students need to borrow the following from the stockroom:

Answer 17

(2) 100-mL beaker
(1) stirring rod
distilled water in wash bottles