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MT6320 > [LAB] Exercise 8B: AST: Broth Macrodilution or Macrobroth Dilution > Flashcards

[LAB] Exercise 8B: AST: Broth Macrodilution or Macrobroth Dilution Flashcards

1
Q

what type of dilution is one of the conventional testing methods used in AST?

A

Broth dilution

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2
Q

This is a technique that uses a standardized amount of test inoculum present in Mueller Hinton Broth (MHB) and a selected antimicrobial agent.

A

Broth dilution

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3
Q

What standardized test inoculum is used in Broth dilution?

A

Mueller Hinton Broth and Selected antimicrobial agent

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4
Q

T/F: The antimicrobial agent is diluted five-fold in the test.

A

F.
two-fold in the test only

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5
Q

T/F: The minimum inhibitory concentration (MIC) is expressed in mg/dl

A

F.
µg/mL

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6
Q

This is the lowest concentration of antimicrobial agent that completely inhibits visible growth

A

Minimum inhibitory concentration (MIC)

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7
Q

T/F: The MIC can be detected semi-automated

A

trew

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8
Q

MIC can be detected v_______, a_______, or s__________ method

A

visually
automated or semi-automated

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9
Q

Name three interpretative categories once MIC has been determined

A

Susceptible (S)
Intermediate (I)
Resistant (R)

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10
Q

Name two categories of broth dilution

A

Broth microdilution (microbroth dilution)
Broth macrodilution (macrobroth dilution)

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11
Q

T/F: Main difference between broth microdilution and broth microdilution is on the volume of the MIC

A

F.
Volume of the MHB used dapat :)

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12
Q

T/F: In exercise 8B, broth microdilution was used.

A

F.
Broth macrodilution
(check the title of the experiment for validation <3) HAHAHA

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13
Q

T/F: Sterile 12mm x 100mm test tubes with screw caps were used in this experiment.

A

F.
13mm x 100mm dapat :)

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14
Q

Yes/No: In this experiment 8B, do we need to standardize the inoculum to a 0.5 McFarland Standard?

A

Yes.

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15
Q

T/F: The test antibiotic used in this experiment was Amoxicillin 20 U/mL

A

F.
Penicillin (working solution of 20 U/mL)

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16
Q

T/F: The test culture must grow on BAP

A

T.

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17
Q

T/F: For other test antibiotics a 200 µg/mL should be your working solution

A

T.

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18
Q

The adjusted inoculum is then diluted to what ratio?

A

1:200

19
Q

T/F: The adjusted inoculum is done by adding 0.1 mL of the standardized inoculum to 19.9 mL of MHB Broth.

A

T.

20
Q

T/F: In the preparation and standardization of the Inoculum, the final bacterial concentration is approximately 4 x 10^5 CFU/mL

A

T.
5 x 10^5 CFU/mL

21
Q

In the test proper, test tube 1-10 will be used for the ______?

a. actual test
b. control tubes

A

a. actual test

22
Q

In the test proper, test tube 11-13 will be used for the ______?

a. actual test
b. control tubes

A

b. control tubes

23
Q

In the test proper, control tube - test tube 11 is the ______?

a. antibiotic control (AC)
b. broth control (BC)
c. inoculum control (IC)

A

c. inoculum control (IC)

24
Q

In the test proper, control tube - test tube 12 is the ______?

a. antibiotic control (AC)
b. broth control (BC)
c. inoculum control (IC)

A

b. broth control (BC)

25
Q

In the test proper, control tube - test tube 13 is the ______?

a. antibiotic control (AC)
b. broth control (BC)
c. inoculum control (IC)

A

a. antibiotic control (AC)

26
Q

In the test proper, the test tubes should be incubated at what degree Celsius?

A

35°C - 37°C

27
Q

T/F: Mix each tube and incubate them at 35-37°C ambient air for 18-24 hrs.

A

T.

28
Q

T/F: We observe visible growth in the form of TURBIDITY

A

T.

29
Q

T/F: prior to interpretation, observe if the ACTUAL TEST are working properly

A

F.
CONTROL TUBES

30
Q

T/F: Test tube 11 - must show VISIBLE GROWTH

A

T.
because inoculum control

31
Q

Test tube 12 - must show VISIBLE GROWTH or NO VISIBLE GROWTH?

A

NO VISIBLE GROWTH
because broth control

32
Q

Test tube 13 - must show VISIBLE GROWTH or NO VISIBLE GROWTH?

A

NO VISIBLE GROWTH
because antibiotic control

33
Q

T/F: In determining the MIC, we determine the tube with the HIGHEST antibiotic concentration

A

F.
LOWEST

34
Q

In determining the MIC, we determine the tube with the lowest antibiotic concentration showing _______? (visible growth or no visible growth)

A

no visible growth

35
Q

T/F: In the determination of MBC, we pipette 0.01 mL from each test tube with no visible growth and subculture to individual CAP plates

A

F.
BAP plates

36
Q

T/F: same with MIC, we incubate the plates for 18-24 hrs at 35-37°C ambient air

A

T.

37
Q

MIC or MBC: It has the lowest antibiotic concentration, which results in 99% killing of organisms.

A

MCB (Minumum Bactericidal Concentration)

38
Q

MIC or MBC: It can be determined by identifying the plate with the lowest antibiotic showing no visible growth

A

MCB (Minumum Bactericidal Concentration)

39
Q

T/F: In broth macrodilution test, fastidious bacteria are required

A

T.

40
Q

The breakpoint of growth inhibition is visible in what tube/s?
(choose only 2 tubes from tubes 1-10)

A

between tube 5 and tube 6

41
Q

What is the main difference between Broth microdilution test and Broth macrodilution test?

A

Volume of MHB (Mueller-Hinton Broth)

42
Q

MIC or MBC: Lowest concentration that prevents the in vitro growth of bacteria

A

MIC (Minimum Inhibitory Concentration)

43
Q

MIC or MBC: Test tube that shows the lowest concentration without any colony growth

A

MBC (Minimum Bactericidal Concentration)

MT6320 (8 decks)

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