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MT6320 > [LAB] Exercise 10A: Throat Culture > Flashcards

[LAB] Exercise 10A: Throat Culture Flashcards

1
Q

A diagnostic test to detect presence of microorganisms in the upper respiratory tract

A

Throat culture/Throat Swab

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2
Q

The most important pathogen causing strep throat

A

Streptoccocus pyogenes

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3
Q

What microorganism can also be recovered and is associated with tonsillar abscess?

A

Staphyloccocus aureus

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4
Q

What other indigenous flora can be detected in throat culture?

A

Viridans streptococci
Beta hemolytic strep (Group A,B,C)
Staphyloccoci
Haemophilus
Moraxella
Anaerobic Bacteria (Clostridium spp.?)

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5
Q

Gram-negative coliform bacteria that is also detected in throat culture

A

Klebsiella

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6
Q

A sterile tongue depressor is used to apply pressure on the tongue to visualize what?

A

tonsillar fossae and posterior pharynx

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7
Q

In the collection of throat swab specimens, 1-2 sterile swabs is extended between?

A

Tonsillar pillars (golden arches) and behind the uvula

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8
Q

Which sites should we avoid in collecting throat swab specimen?

A

Tongue
Buccal mucosa
Uvula

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9
Q

Rub the sterile swabs back and forth across the?

A

Posterior pharynx
Tonsillar areas
Any inflamed or exudative areas

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10
Q

T/F: Do not collect specimens if the epiglottis is inflamed

A

True

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11
Q

Collecting specimens from an inflamed epiglottis can lead to?

A

Respiratory obstruction

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12
Q

Where are swabs placed after collection?

A

Sterile tube or transport media

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13
Q

T/F: All culture media (BAP, CAP, PEA, EMB) should be brought at 37C prior to inoculation

A

False
Room temperature

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14
Q

Firmly roll the swab on to BAP over ____ portion of the BAP surface

A

1/6th

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15
Q

T/F: Using the same swab from BAP, the swab is rolled as well into CAP, PEA, and EMB.

A

True

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16
Q

What streaking method is used?

A

Isolation/four quadrant method

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17
Q

Where is the Taxo A disk placed in the inoculated BAP?

A

Junction of 1st and 2nd quadrants

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18
Q

How much is the distance of Taxo A disk from the edge?

A

10-mm from the edge

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19
Q

What is the component of Taxo A Disk?

A

0.04 units Bacitracin

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20
Q

Inoculated plates are incubated at what temperature?

A

37C

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21
Q

What is the incubation time for the inoculated plates?

A

18-24 hours

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22
Q

Growth can be further enhanced if the inoculated BAP and CAP are incubated with?

A

Increased CO2

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23
Q

What can be done to improve detection of hemolysis?

A

After streaking, carefully stab the BAP several times using the same loop in the streaked and unstreaked areas

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24
Q

In which media can we observe for isolated pinhead colonies and pinpoint colonies that are entire, white or yellow, clear or opalescent, and creamy in appearance?

A

BAP

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25
Q

T/F: Other colonies that may be present include large, grayish mucoid/slimy colonies

A

True

26
Q

In which media can we observe colonies that are similar to BAP but no hemolytic patterns?

A

CAP

27
Q

T/F: We also observe for pinpoint and pinhead colonies in PEA

A

True

28
Q

Colonies growing on this medium is presumptive as gram-positive cocci

A

PEA

29
Q

T/F: Growth is usually seen in EMB

A

False;
No growth is usually seen

30
Q

In which media can we observe large purple or colorless colonies if growth is seen

A

EMB

31
Q

T/F: It is susceptible if there is no zone of inhibition

A

False

32
Q

Interpretation if there is any zone of inhibition

A

Susceptible

33
Q

Interpretation if there is no zone of inhibition

A

Resistant

34
Q

In the Catalase Test, from which media do we fish 1-2 similar looking isolated pinhead and pinpoint colonies?

A

PEA or CAP

35
Q

In the Catalase Test, what is added to the slides mixed with the colonies?

A

1-2 drops of 3% Hydrogen Peroxide

36
Q

Positive result of Catalase Test

A

Bubbling or effervescence

37
Q

In which media is the colony inoculated from in Gram stain?

A

BAP or CAP

38
Q

T/F: Proceed to the succeeding procedure if you obtained a negative catalase test

A

False
Do not proceed

39
Q

T/F: If you obtained a positive catalase test, proceed to Coagulase Testing

A

True

40
Q

What media is the specimen inoculated from in coagulase test?

A

BAP or CAP

41
Q

In the slide coagulase test what is first dropped on a clean grease-free slide?

A

NSS

42
Q

After emulsifying colonies in slide coagulate testing, what is added next?

A

1-2 drops of rabbit plasma

43
Q

T/F: In the slide coagulase test, the slide is placed on a dark/black cardboard and tilted when observing for macroscopic clumping

A

True

44
Q

How long do we observe for macroscopic clumping in slide coagulase test

A

Around 10 seconds

45
Q

When is the test considered negative for Slide Coagulate Test?

A

No clumping occurs after 2 minutes

46
Q

What test tube is used for Tube Coagulase Test?

A

Kahn test tube

47
Q

In tube coagulase test, how much rabbit plasma is placed on the Kahn test tube?

A

0.5mL

48
Q

T/F: Pooled human plasma can be used if rabbit plasma is unavailable in Tube Coagulase Test

A

True

49
Q

For how long should we incubate the tube coagulase test sample when observing for clots/coagulum?

A

4 hours

50
Q

In what temperature should the tube coagulase test be incubated at?

A

37C

51
Q

What is the positive result for tube coagulase test?

A

Clot or coagulum formation

52
Q

If no clot is seen after 4 hours, what should be done before reporting the tube coagulase test as negative?

A

Incubate at room temperature for 20 hours

53
Q

In Mannitol Fermentation Test, from what media are the 2-3 colonies fished out from?

A

PEA or BAP

54
Q

In Mannitol Fermentation Test, what streaking method is used?

A

Simple streaking

55
Q

In Mannitol Fermentation Test, what is the incubation time and temperature?

A

24 hours, 37C

56
Q

Mannitol Fermenter/Non-Mannitol Fermenter: Colonies are yellow and plate becomes yellow

A

Mannitol Fermenter

57
Q

Mannitol Fermenter/Non-Mannitol Fermenter: Pink to red plates

A

Non-mannitol fermenter

58
Q

Other tests can be performed on which colonies?

A

Catalase negative and are gram positive cocci in chains

59
Q

What is resistant to Taxo P (Optochin)?

A

Viridans streptococci (alpha hemolytic)

60
Q

T/F: S. pyogenes is PYR postive

A

True

61
Q

T/F: S. pyogenes is susceptible to SXT-TMP

A

False
Resistant

MT6320 (8 decks)

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