[LAB] Exercise 8A: E-Test

Question 1

T or F: The E-test is a qualitative assay for determining the MIC

Answer 1

FALSE- E-test is a QUANTITATIVE assay

Question 2

E-test Principle order:
(1) The preformed exponential gradient of the antimicrobial agent is transferred to the agar
(2) MIC is read
(3) The test strip is applied to the innoculated agar surface
(4) A symmetrical inhibition ellipse centered along the strip is formed

Answer 2

(3) The test strip is applied to the innoculated agar surface
(1) The preformed exponential gradient of the antimicrobial agent is transferred to the agar
(4) A symmetrical inhibition ellipse centered along the strip is formed
(2) MIC is read

Question 3

The E-test is a ___________ diffusion method

Answer 3

GRADIENT

Question 4

In gradient diffusion method, the e-test uses a ______ strip, and a liofilchem uses a _______ strip

Answer 4

plastic, paper

Question 5

Set up time for E-test should be:

Answer 5

<5 minutes

Question 6

Time to result of E-test:

Answer 6

16 to 20 hrs

Question 7

The strip can be repositioned within ________ minutes of application

Answer 7

3 minutes

Question 8

T or F: Observe where the relevant inhibition ellipse INTERSECTS the strip and read the MIC

Answer 8

True

Question 9

T or F: An inhibition ellipse that intersects below the lower end of the scale is read as More than (>) the lowest value

Answer 9

False: Less than (<) the lowest value