Lab Exercise #17: DNA Fingerprinting Flashcards
What was the agarose for
It’s a gel-like substance was used to hold the loading dye for gel electrophoresis
What was the buffer used for
Serves both as a conductor of electricity and to control pH
Restriction enzymes
Enzymes used to cut recognized tandem repeat segments from the DNA of a specific chromosome
Restriction Fragment Length Polymorphisms (RFLP)
The variation in the lengths of fragments cut by a restriction enzyme
Tandem Repeats
A series of repeated bases on a chromosome
Noncoding DNA
The rest of the DNA that is not genes
coding DNA
1-5% of the human DNA that contains specific instructions for making proteins
Gel electrophoresis chamber
The gel is placed in an electrophoresis chamber, which is then connected to a power source. When the electric current is applied, the larger molecules move more slowly through the gel while the smaller molecules move faster.
Single Locus Tandem Repeats
When only one set of tandem repeats is analyzed
Which direction will DNA move in the chamber? Why?
It will move towards the positive pole since the fragments are negatively charged
Why are there 2 bands in each lane of DNA fragments?
The repeat sequence is located on each of a pair of homologous chromosomes in diploid organisms
What is the difference between coding and noncoding DNA? Which one is better for finding differences among human DNA?
Coding DNA is the 1-5% human genes that offers little difference. Noncoding DNA is the rest of the DNA and where differences accumulate.
How are restriction enzymes used in gel electrophoresis?
it was used to separate the samples of DNA
Why is this technique called “DNA Fingerprinting”?
It is an identifying technique. Not an actual use of fingerprints
