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Biol-31 > lab exam 1 > Flashcards

lab exam 1 Flashcards

1
Q

What is selective media?

A

Inhibits a type of organism
Promotes growth of another
-looking for any growth

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2
Q

what is differential media?

A

Differentiates bacteria by their metabolic processes.
- COLOR change

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3
Q

what is chemically defined media?

A

the chemical components of the media are known in detail
- tailored for an organism

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4
Q

what is complex media?

A

media is diverse in components
- very general usage

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5
Q

what are the three types of hemolysis? please differentiate between each

A
  1. Alpha (incomplete)
  2. Beta (complete)
  3. Gamma (Non-existent)
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6
Q

What is considered an acidic stain?

A

A dye which has a negative chromophore (colored ion)

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7
Q

what is considered a basic stain?

A

A dye which has a positive chromophore (colored ion)

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8
Q

What is the difference between differential and simple stains?

A

Differential uses multiple stains
Simple uses one stain

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9
Q

what are the three levels of motility?

A
  1. True motility (propels itself)
  2. Brownian movement ( moves with the current)
  3. Non-motile
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10
Q

What are the placements of the flagella? List them.

A
  1. Monotrichous
  2. Amphitrichous
  3. Lophotrichous
  4. Peritrichous
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11
Q

What does HE stand for in the HE media?

A

Hektoen Enteric

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12
Q

In HE media, what is the selective chemical and what does it promote as well as inhibit?

A

Selective chemical: ferric ammonium citrate
promotes: gram - enteric
inhibits: gram +

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13
Q

In HE media what is the differential chemical?

A

Lactose

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14
Q

In HE media, what is the pH indicator?

A

bromethylmol blue

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15
Q

What can we assume about a yellow/salmon colony growing on HE media?
guess organism too

A

It is a gram - enteric species
it can ferment lactose
organism: ecoli

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16
Q

What can we assume about a green colony with a black center in HE?
organism?

A

it is a gram - enteric species
it can ferment lactose
organism: salmonella

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17
Q

What can we assume about a yellow colony in HE?
organism?

A

it is a gram - enteric species
it cannot ferment lactose
organism: enterobacter

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18
Q

What is HE media used to screen for?

A

Screening stool samples

19
Q

What does EMB stand for in the EMB media?

A

Eosin Methylene-Blue

20
Q

what is the selective chemical in EMB? what does it promote/inhibit?

A

Eosin methylene blue
promotes gram -
inhibits gram +

21
Q

what is the differential chemical in EMB?

A

lactose

22
Q

what does a metallic green colony represent in EMB?
organism?

A

it is a gram - bacteria
it can ferment lactose
organism: ecoli

23
Q

What does a yellow colony or a colony with no significant color different represent in EMB?
organisms?

A

It is gram -
no lactose fermentation
staph and klebsiella

24
Q

What is EMB media used to screen for?

A

Looking for feces in water in an environmental setting
-think for contamination in big water tanks

25
Q

what is the selective chemical in rose agar?
what does it promote/inhibit?

A
  1. colistin
    2.nalidixic acid
  2. PEA
    it promotes gram + and inhibits gram -
26
Q

what is the differential chemical in rose agar?

A

sheeps blood

27
Q

what is rose agar screen for?

A

hemolytic activity

28
Q

what would colonies with a clear halo on a rose agar indicate?
organism?

A

staph aureus - beta/complete hemolysis
gram +

29
Q

what would colonies with a green halo on a rose agar indicate?
organism?

A

enterococcus faecalis - alpha/incomplete hemolysis
gram +

30
Q

what is the selective chemical in Mac-Conkey agar?
what does it promote/inhibit?

A

crystal violet and bile salts
promotes gram -
inhibits gram +

31
Q

what is the differential chemical in Mac-Conkey agar?

A

lactose

32
Q

What does Mac-Conkey agar screen for?

A

urine and stool samples in the clinical setting
-think of healthcare settings

33
Q

what does bright fuchsia pink colonies indicate on a Mac-Conkey plate?
organism?

A

gram - bacteria and lactose fermenter
Ecoli

34
Q

what is the selective chemical in mannitol salt?
-inhibits?
-promotes?

A

7.5% NaCl
promotes halophiles (high salt)
inhibits halo-phobic bacteria

35
Q

what is the differential chemical in mannitol salt?

A

mannitol

36
Q

what is the pH indicator of mannitol salt?
what happens if it becomes more acidic?

A

phenol red
more acidic–> lower pH–> more yellow

37
Q

what does a colony with a yellow halo indicate on a mannitol salt plate? organism?

A

staphylococcus - halophile + mannitol fermenter

38
Q

what does m.l. looks like on plate?

A

bright yellow
smooth
circular
convex

39
Q

what does P.A. look like on a plate?

A

GREEEN/ METALLIC GREEN
undulate
irregular

40
Q

what does B.C. look like on a plate?

A

grey/white
irregular
large feathery pattern

41
Q

what does M.S. look like on a plate?

A

smooth
raised
waxy, wrinkled

41
Q
A
42
Q
A
42
Q
A

Biol-31 (12 decks)

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