Lab: Bright field, Phase contrast, and Fluorescence Microscopy

Question 1

3 typical bacterial morphologies

Answer 1

1. chains (streptococci)
2. clusters (staphylococci)
3. diplococcus (pairs of balls)

Question 2

What is a bright field microscope?

Answer 2

allows light rays to pass directly to the eye without deflection from an opaque plate in the condenser

Question 3

What does a condenser do?

Answer 3

collects and directs light from the lamp to the slide

Question 4

Bright field microscopes are parfocal which means

Answer 4

you can change from a lower objective to a higher objective while keeping the image in focus

Question 5

In Bright field microscopy, the sample must be ________ in order to see it

Answer 5

stained

Question 6

How is total magnification found?

Answer 6

objective * ocular

Question 7

What are the 4 types of objectives?

Answer 7

4x/5x rapid scanning
10x low power
40x/50x high power
100x oil immersion

Question 8

What is a phase contrast microscope?

Answer 8

Able to differentiate transparent structures of a microorganism and enhance the contrast between the cell and its surroundings through the use of an opaque disc in the condenser

Question 9

With a phase contrast microscope the specimen can be ________

Answer 9

living

Question 10

What is a fluorescent microscope?

Answer 10

Used primarily in the immune-medical field and microbial ecology. One of the most common approaches used in studying the location and movement of molecules and cellular components within the cell.