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Lab: Bacteria Flashcards

0
Q

Gram stain of E. coli

A

Gram (-)

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1
Q

What is the morphology of E. coli?

A

Bacilli

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2
Q

Colony appearance of E. coli

A

Beige colonies

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3
Q

Details of E. coli

A
  • most commonly worked with bacteria
  • most strains harmless, typically hear about O157:H7 that causes food poisoning
  • ubiquitous
  • part of Enterobacteriaceae family
  • molecular “workhorse”
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4
Q

What’s the morphology of S. aureus?

A

Cocci

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5
Q

Gram stain of S. aureus

A

Gram +

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6
Q

Colony appearance of S. aureus

A

Golden yellow

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7
Q

Details of S. aureus

A
  • golden grape bunches
  • normal part of skin flora but typically hear about MRSA
  • staph typically blamed for Strep infections
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8
Q

Morphology of S. mutans

A

Cocci

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9
Q

Gram stain of S. mutans

A

Gram +

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10
Q

Colony appearance of S. mutans

A

Opaque colonies

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11
Q

Details of S. mutans

A
  • normal part of oral flora
  • responsible for carb metabolism thus creating an acidic environment that contributes to dental caries
  • forms dental biofilms
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12
Q

Morphology of P. hauseri

A

Bacilli

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13
Q

Gram stain of P. hauseri

A

Gram -

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14
Q

Colony appearance of P. hauseri

A

Opaque colonies

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15
Q

Details of P. hauseri

A
  • highly motile
  • found in intestines of humans and animals, soil and water
  • known to cause UTIs
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16
Q

Morphology of B. cereus

A

Bacilli

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17
Q

Gram stain of B. cereus

A

Gram +

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18
Q

Colony appearance of B. cereus

A

Fluffy white colonies

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19
Q

Details of B. cereus

A
  • often used as probiotic for animals
  • harmless except for strains that cause fried rice syndrome
  • can produce endospores
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20
Q

Morphology of B. megaterium

21
Q

Gram stain of B. megaterium

22
Q

Colony appearance of B. megaterium

A

White with ruffled edges

23
Q

Details of B. megaterium

A
  • one of largest bacteria in terms of size
  • naturally produces penicillin amidase
  • can produce spores
24
Q

Morphology of B. subtilis

25
Q

Gram stain of B. subtilis

26
Q

Colony appearance of B. subtilis

A

Dull, tan, raised, wrinkled edges

27
Q

Details of B. subtilis

A
  • found in soil and vegetation
  • model organism for the study of endospore formation
  • can be used as fungicide
  • naturally produces new phospholipid antibiotic
  • degrades TNT
28
Q

Morphology of K. rosea

29
Q

Gram stain of K. rosea

30
Q

Colony appearance of K. rosea

A

Pink colonies

31
Q

Details of K. rosea

A
  • aka Micrococcus roseus
  • found in freshwater, saltwater and soil
  • chromogenesis at 25 to form pink colonies
  • fastidious
32
Q

Morphology of M. luteus

33
Q

Gram stain of M. luteus

34
Q

Colony appearance of M. luteus

A

Yellow colonies

35
Q

Details of M. luteus

A
  • aka Kocuria rhizophila
  • typical soil bacterium
  • threat to immunocompromised individuals
  • chromogenesis at 25 to form yellow colonies
36
Q

Morphology of S. marcescens

37
Q

Gram stain of S. marcescens

38
Q

Colony appearance of S. marcescens

A

Red colonies

39
Q

Details of S. marcescens

A
  • produces prodigiosin at 25 giving red appearance
  • very ubiquitous
  • can be pathogen (UTI and catheter)
  • US and UK tested effects by spraying
  • church wafers
40
Q

Morphology of P. aeruginosa

41
Q

Gram stain of P. aeruginosa

42
Q

Colony appearance of P. aeruginosa

A

Opaque green/blue colonies

43
Q

Details of P. aeruginosa

A
  • ubiquitous human and plant pathogen
  • produces pyocyanin (blue-green) at 37
  • loves to be in biofilms
  • CF and burn patients
44
Q

Morphology of E. faecalis

45
Q

Gram stain of E. faecalis

46
Q

Colony appearance of of E. faecalis

A

Opaque colonies

47
Q

Details of E. faecalis

A
  • typical GI bacterium
  • found in cosmetics and hygienic chemicals
  • ability to spoil sap and syrup
  • part of Enterobacteriaceae family
48
Q

Morphology of P. phosphoreum

49
Q

Gram stain of P. phosphoreum

50
Q

Colony appearance of P. phosphoreum

A

Blue-green glowing colonies

51
Q

Details of P. phosphoreum

A
  • highly motile
  • symbiotic, often found in deep sea fish
  • used to assess biological toxicity within a system

Microbiology (22 decks)

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