Lab: Biochemical testing I: Sugar fermentation, oxygen requirements, motility determination

Question 1

Sugar fermentation

Answer 1

• produces acid which changes phenol red to change broth to yellow
• not all sugar fermenters produce gas

Question 2

FTM

Answer 2

• fluid thioglycollate medium
• assesses oxygen reuqirements
• has high conc. of O2 at top and no O2 at bottom

Question 3

Chemotaxis

Answer 3

movement that allows bacteria away from harmful substances and towards nutrients

Question 4

polar flagellum

Answer 4

single flagellum at one end

Question 5

lophotrichous flagella

Answer 5

multiple flagella at one end

Question 6

bipolar/amphitrichous flagella

Answer 6

single or tufts of flagella at both ends

Question 7

peritrichous flagella

Answer 7

multiple flagella all over cell

Question 8

Ways to test motility

Answer 8

• wet mount
• hanging drop
• semi-solid media
• SIM (sulfur reduction, indole production, and motility)