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Bio 153 (MIDTERM LAB EXAM) > Lab #6 > Flashcards

Lab #6 Flashcards

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Biology 101 flashcards
1
Q

what is colony morphology

A

-with the naked eye: shape, edge, surface & elevation

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2
Q

What is coccus

A

round

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3
Q

what is bacillus

A

rod

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4
Q

what is spirillum

A

curved/coiled

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5
Q

what is diplo

A

2

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6
Q

what is strepto

A

chain

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7
Q

what is staphylo

A

cluster

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8
Q

Purple cells

A

gram +, rich in peptidoglycan

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9
Q

pink cells

A

gram -, rich in lipopolysaccharide

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10
Q

what does crystal violet do

A

stains peptidoglycan (especially in gr. +)

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11
Q

What does grams iodine do

A

application of mordant that forms an insoluble complex with crystal violet (fixes stain to cell wall)

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12
Q

What does Acetone-Alcohol do

A

dehydrates cell, traps crystal violet/iodine complex.

In gr.- it dissolves lipopolyssacharaide & washes away excess stain

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13
Q

what does safranin do

A

stains inner peptidoglycan coat for gr-. makes them pink

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14
Q

steps of staining

A

1) Water
2) Bacteria
3) Heatfix
4) crystal violet
5) Rinse
6) Iodine
7) Acetone-iodine
8) rinse
9) safarin
10) Rinse

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15
Q

what is a permissive agar

A

allows growth of anything

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16
Q

what is an indicator agar

A

organisms allows growth of any

17
Q

blood agar plate

A

enriched media used to isolate organisms that require more nutrition
differential to detect hemolytic activity

18
Q

B-hemolytic activity

A

complete lysis (clear zone)

19
Q

Alpha-hemolytic activity

A

only partial lyse (green)

20
Q

Y-hemolysis

A

no hemolytic activity

21
Q

what is blood agar good for

A

differentiating streptococcus from staphylococcus

22
Q

what kind will grow on macconkey agar

A

gram -, enteric bacteria

if it can eat lactose it shows its a gut bacteria

23
Q

MAC agar contains…

A

bile salts (inhibit non enteric)
crystal violet dye (inhibit gram + bacteria)
neutral red dye (stains microbes fermenting lactose)
Lactose
Peptone

24
Q

what does mac agar distinguish

A

lac + from lac -

25
Q

If a bacteria is Lac +…

A

it means its enteric & it’ll be pink/ red

it produces acid that lowers the ph

26
Q

if the bacteria is lac-

A

it means it cannot utilize peptone, forms ammonia raises ph so colonies are white

27
Q

what is macconkey useful for

A

differentiating between salmonella & coli

28
Q

what are aerobic bacteria

A

need O2 to grow

29
Q

what are anaerobic bacteria

A

don’t need O2 to grow

30
Q

no bacteria on MAC

A

bacteria are gram +, non-enteric or both

cannot grow b/c bile salts & crystal violet

31
Q

what is MRSA

A

methicillin resistant staphylocccus aureus

32
Q

what is VRE

A

vancomycin-resistant enteroccocus ( can live a long time on other objects too)

Bio 153 (MIDTERM LAB EXAM) (6 decks)

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