Lab 4: Microscopy And The Cell

Question 0

How long has life existed on Earth??

Answer 0

At least 3.5 billion years.

Question 1

When was the microscope invented??

Answer 1

1590

Question 2

Prokaryotic cell??

Answer 2

Most primitive cell type. Lack membrane bound nucleus and organelles. Later evolved into eukaryotic cells.

Question 3

Eukaryotic Cells??

Answer 3

Do have a nucleus and membrane-bound organelles.

Question 4

List the 3 domains of life on Earth??

Answer 4

1: eukarya
2: archaea
3: bacteria.

(2-3 are prokaryotic cells)

Question 5

What microscopes were utilized during the experiment?

Answer 5

1: Compound Microscope
2: Dissecting Microscope

Question 6

Define Compound Microscope??

Answer 6

Microscope that uses at least 2 lenses in cooperation to magnify an object.

Question 7

Describe components of a COMPOUND MICROSCOPE??

Answer 7

1: HEAD
-Ocular Sense (10x magnification)
-Camera Attachment Tube
-Revolving Nosepiece
- 4 Objective Lenses
A) 4x scanning lens
B) 10x objective lens
C) 40x high-dry lens
D) 100x
2: ARM
-Stage
A) mechanical stage
B) condenser
C) iris diaphragm
D) condenser adjust.
E)mech. stage adjust
-Light switch
-light intensity knob

3: BASE
-sub stage light
-coarse focus knob
A) fine focus knob

Question 8

Define DISSECTING SCOPE??

Answer 8

(STEREOSCOPE) used to show detail, but object is too large/thick for Compound Scope.

Question 9

Define ARCHAEA??

Answer 9

Extremophiles prokaryotes.

Question 10

Thermus Aquaticus

Answer 10

Activity 1: Archaea. It’s Taq enzymes are used to recreate millions of DNA without cells due to the enzymes not denaturing. Purple dots.

Question 11

Anabaena.

Answer 11

Activity 2: Photosynthetic Bacteria. Green rings.

Question 12

Physarum??

Answer 12

Activity 3: Slime mold of Protists.

Question 13

Paramecium??

Answer 13

Single-celled animal-like Protists.

Question 14

Depression Slide??

Answer 14

Slide with a well in it.

Question 16

List Domain Eukarya Kingdoms??

Answer 16

1: Protista
2: Plantae
3: Fungi
4: Animalia

Question 17

List members of EUKARYA??

Answer 17

1: land plants
2: algae
3: animals
4: fungi
5: ciliates
6: diatoms
7: euglena

Question 18

List members of ARCHAEA KINGDOM??

Answer 18

1: halophiles
2: thermophiles
3: sulfolobus
4: methanobacterium

Question 19

List members of KINGDOM BACTERIA??

Answer 19

1: mitochondrion
2: Cyanobacteria
3: chlamydia
4: spirochetes
5: green sulfur/nonsulfur bacteria.

Question 20

Procedure for procuring and using Microscopes??

Answer 20

1: retrieve with 2 hands.
2: wipe dust off all glass with LENS PAPER
3: lower stage all the way down.
4: objective lens at lowest setting.
5: place slide on stage and secure.
6: adjust using mech. stage adjustor
7: rotate Course Focus knob.

Question 21

Difference of animal/plant cells??

Answer 21

1: chloroplast
2: cell wall
3: vacuole

Question 22

Endosymbiotic Theory??

Answer 22

States that eukaryotic organelles evolved from aerobic prokaryotes.

Question 35

Describe components of a DISSECTING MICROSCOPE??

Answer 35

1: OCULARS
2: OBJECTIVE
-incident light
-focusing knob
3: BASE
-on/off switch
-stage
A) transmitted light