lab 4 Flashcards
what cell lines were used and why
raji cells lines as they highly e4xpress CD20
What shows apoptosis
- cell shrinkage
- chromatin condensation
- nuclear fragmentation
what shows necrosis
- cell swelling which leads to rupturing of the cell
how was flourcsent microspy used
looked at the effcet of rutximab in changes on nuclear morphology
what is the dye for flourcsent microspy and how does it work
acridine orange
- interacts with DNA by intercalation
- interacts with RNA through electrostatic attraction
what does SRB stand for
sulphorhodamine B
how does srb work
relies on the negative charges of pink aminoxanthine dye binding to basic AA in cells
in SRB how do you know if there has been cell death
will appear less pink
what do you assume about the control in SRB
that there has been no cell death
how to lay out an SRB
use incraesing conc of Ab 1, 3, 30 , 100
what is the efficacy of the drug
- maxium reposne prodced by drug
explain potency
it teh conc needed to get wanted affect
e.g. LD 50
explain differneces in epitopes in oftaumab and ritiuximab and obinutuzumab
oftaumab & ritiuximab no over alp
ritiuximab & obinutuzumab partial overlap
what does a compeitive antagonist do
interacts with recpetors to reduce potency
what does a non competeive antagonist do
interacts with differnet site cuasing conformational changes reudcing efficacy
