epitope mapping Flashcards

1
Q

what is an epitope

A

a molecular strcuture within an antigen that makes specific conatct with antibody

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2
Q

what does it is called when the aa for an epitope are disconitous

A

a conformational epitope or discontinuos epitope

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3
Q

what is it called when aa are lined up in an epitope

A

linear epitope or continousepitope

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4
Q

what different techniques are used to epitope map

A
  • xray crystallography
  • cryo em
  • array based oglio nucleotide scanning
  • site directed mutagenesis mapping
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5
Q

what are the three epitopes for CD20

A

oftuzumamb
ritixiumab
GA101

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6
Q

why is epitope mapping important

A

plays a key role in fucntioanl undertsanding and selection of antibodies, patent protection and medical body approval

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7
Q

what are CLIPs and how are they useful

A

they pin small sections of peptide structures to create a 3d structure
this improves siganals, mimics the structure taht would be found in nature and allows the idnetification of more aas

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8
Q

what bacterial infections are mAbs looking to treat

A

staphlococcus areus
staphlococcus epiderimus

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9
Q

what virus’s are mAbs looking to treat

A

HIV-1
ebola
covid

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10
Q

how many mAbs are there avaible for MHRA

A

8

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11
Q

WHAT mAb is used to treat anthrax

A

Raxibacumab

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12
Q

what is a mAb used to treat Ebola

A

Zmapp

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13
Q

why is HIV-1 so hard to make mAbs

A

its reverse transcriptase creates lots of error

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14
Q

why is it easier to make mAbs of SARS2

A

RNA dependant RNA polymerase this proof reads so less error

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15
Q

what is fine epitope binding

A

its define the epitope to a single residue level

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16
Q

what is site directed mutagensis used for

A

amino acid replcement scanning (replacemnet of a single aa)

17
Q

what are te advantges of site directed muatgensis

A
  • identifies key aa
  • can result in increasing binding affininty
  • gives information in thermodynamics of epitope-paratope binding
  • enables the development of superior peptide binders as potential inhibitoirs of protein activity
18
Q

what mAb helps with the symptoms of COVID

A

Sotrovimab

19
Q

what is a paratope

A

part of the antibody that binds to te epitope on the antigen