epitope mapping Flashcards
what is an epitope
a molecular strcuture within an antigen that makes specific conatct with antibody
what does it is called when the aa for an epitope are disconitous
a conformational epitope or discontinuos epitope
what is it called when aa are lined up in an epitope
linear epitope or continousepitope
what different techniques are used to epitope map
- xray crystallography
- cryo em
- array based oglio nucleotide scanning
- site directed mutagenesis mapping
what are the three epitopes for CD20
oftuzumamb
ritixiumab
GA101
why is epitope mapping important
plays a key role in fucntioanl undertsanding and selection of antibodies, patent protection and medical body approval
what are CLIPs and how are they useful
they pin small sections of peptide structures to create a 3d structure
this improves siganals, mimics the structure taht would be found in nature and allows the idnetification of more aas
what bacterial infections are mAbs looking to treat
staphlococcus areus
staphlococcus epiderimus
what virus’s are mAbs looking to treat
HIV-1
ebola
covid
how many mAbs are there avaible for MHRA
8
WHAT mAb is used to treat anthrax
Raxibacumab
what is a mAb used to treat Ebola
Zmapp
why is HIV-1 so hard to make mAbs
its reverse transcriptase creates lots of error
why is it easier to make mAbs of SARS2
RNA dependant RNA polymerase this proof reads so less error
what is fine epitope binding
its define the epitope to a single residue level
what is site directed mutagensis used for
amino acid replcement scanning (replacemnet of a single aa)
what are te advantges of site directed muatgensis
- identifies key aa
- can result in increasing binding affininty
- gives information in thermodynamics of epitope-paratope binding
- enables the development of superior peptide binders as potential inhibitoirs of protein activity
what mAb helps with the symptoms of COVID
Sotrovimab
what is a paratope
part of the antibody that binds to te epitope on the antigen
