Lab 4 Flashcards
how many membranes does a mitochondria have
2 membranes, outer and inner (folded into cristae)
how many membranes does a chloroplast have
3 membranes, outer, inner, and internal (thylakoids)
how to identify vacuoles in TEM
single membrane, fairly empty
how to identify nucleus in TEM
double membrane, grainy, possible nucleoli
how to identify chloroplasts in TEM
double membrane, internal membrane, grana
how to identify chromoplasts and plastids in TEM
double membrane
how to identify mitochondria in TEM
small, look like tomato slices
how to identify golgi in TEM
series of sacs, blurry side view
how to identify ER in TEM
individual sacs/tubes, dots=rough ER
how to identify peroxisomes in TEM
darkly stained circles, near chloroplasts w/ mitochondria
where does respiration start?
in the cytoplasm with glycolysis, produces pyruvate
where in the mitochondria does the ETS cycle occur
on inner membrane
where in the mitochondria does the TCA cycle occur
the matrix
what is the final electron acceptor of ETS that becomes water
O2
tetrazolium experiment
detects ETS activity by forming a red product
no activity in dead corn seeds
thermos experiment findings
ETS and TCA produce heat
CO2/O2 experiment findings
CO2 production decreases
respiration
converts sugar + O2 unto H2O
starting material for sugar production in photosynthesis
CO2
photosynthesis
light is captured, energy stored in ATP and NADPH, used to build sugar
pyrenoids
starch is produced in the chloroplast
where do other plant tissues store starch
plastids (amyloplasts)
what organelles are near chloroplasts and aid in photorespiration
peroxisome and mitochondria
bundle sheath cells
make starch in CO2 rich area
