Lab 4 Flashcards
how many membranes does a mitochondria have
2 membranes, outer and inner (folded into cristae)
how many membranes does a chloroplast have
3 membranes, outer, inner, and internal (thylakoids)
how to identify vacuoles in TEM
single membrane, fairly empty
how to identify nucleus in TEM
double membrane, grainy, possible nucleoli
how to identify chloroplasts in TEM
double membrane, internal membrane, grana
how to identify chromoplasts and plastids in TEM
double membrane
how to identify mitochondria in TEM
small, look like tomato slices
how to identify golgi in TEM
series of sacs, blurry side view
how to identify ER in TEM
individual sacs/tubes, dots=rough ER
how to identify peroxisomes in TEM
darkly stained circles, near chloroplasts w/ mitochondria
where does respiration start?
in the cytoplasm with glycolysis, produces pyruvate
where in the mitochondria does the ETS cycle occur
on inner membrane
where in the mitochondria does the TCA cycle occur
the matrix
what is the final electron acceptor of ETS that becomes water
O2
tetrazolium experiment
detects ETS activity by forming a red product
no activity in dead corn seeds