LAB 3 - Neisseria sp. & Moraxella catarrhalis Flashcards
when N. meningitidis is isolated from invasive infections
- blood, CSF, etc.)
- highly contagious!
lab acquired infections!!
N. meningitidis can be carried in the…. so it can cause infection in the …
can be carried in URT.. cause infection in LRT
the second most commonly reported sexually transmitted infection in Edmonton (behind Chlamydia)
N. gonorrhoeae
where are gonococcal infections localized?
cervix
pharyngeal surface
anorectal area
urethra
conjunctiva
how to confirm gonorrhoeae?
culture or dNA testing
Which organism is susceptible to SPS?
N. gonorrhoeae
M. cat is a common pathogen here
resp tract
oxidase
- should be colourless; turns purple bc light sensitive
- determines bacteria that have cytochrome c oxidase
- never use MAC for oxidase testing or other selective media ; anything w fermentable carbs
common characteristics of Neisseria sp
nonmotile
ox and ct pos
nitrate neg
appropriate specimens for GC
- endocervical, urethral, anorectal, pharyngeal, blood + fluid samples
- swabs should be made of Dacron or rayon bc cotton and calcium alginate = inhibitory
specimen transport of GC
- non-nutritive =
> Stuart’s, Amies
> protect from exposure to O2, toxic fatty acids, and drying; can survive at RT - nutritive =
> Transgrow, JEMBEC, Gono-pack
> culture medium such as MTM and NYC agar to sustain growth of GC plus increases CO2 in case of delays in transit
why are D/S not recommended for pharyngeal specimens where we are wanting to screen for GC
saprophytic Neisseriaare normal colonizers here
superoxol test
for GC
- 30% H2O2 = vigorous bubbling right away
T or F. Gonococci are resistant to high concentrations of penicillin
T!
- cleaves beta-lactam bond of penicillin to form inactive penicilloic acid
- chromogenic cephalosporin nitrocefin (Thronsberry method); pk colour is pos within ten mins
this is usually present in patients with meningococcemia
petechia
often targets children and young adults
