LAB 11 - Anaerobes Flashcards
1
Q
T or F. Most of the anaerobic bacteria that cause infections are also part of the normal flora of the oral cavity, vagina, and gut
A
T~
2
Q
T or F. Most common anaerobic infections are exohenous
A
F! endogenous
3
Q
aerotolerance
A
%
BAP*
CHOC
4
Q
Actinomyces israelii
A
- wt/molar tooth
- branching gpb pleo
- cat neg
5
Q
B. fragilis
A
- shygy, wtgy, gymuc
- gncb
- cat pos
- BBEA +
- K, V, C resistant
6
Q
Prevotella sp./Porphyromonas sp.
A
- brown/black convex; 1-3 wks
- gnb/cb
- cat neg
- BBEA neg
- young colonies = brick red fluorescence
- KV resistant
7
Q
C. perfringens
A
- DZH
- gpb, boxcars
- cat neg
- reverse camp pos
- lec pos
- lipase neg
8
Q
C. sordellii
A
- spr/swr
- gpb, spores
- cat neg
- reverse camp neg
- lecithinase pos
- lipase neg
9
Q
C. sporogenes
A
- tgy/rh (yel center)
- gpb, spores
- cat neg
- reverse CAMP neg
- lec neg
- lipase pos
10
Q
Fusobacterium
A
- v strict anaerobe
- bread crumbs, speck, sm
- gnb, fine, tapered
- cat neg
- indole pos
- UV = chartreuse (pale yel/grn fluorescence)
- V resistant
11
Q
Peptostreptococcus sp.
A
- gy gold, wt op
- gpc, ch
- cat neg
- KC resistant
12
Q
Cutibacterium acnes
A
(prev. Prop)
- tiny wt/ op
- gpb, pleo (club shaped)
- cat pos
- colistin R
13
Q
Veillonella
A
- sm gy
- sm gnc, sheets
- cat V
- nitrate to nitrite
14
Q
what is the purpose of kanamycin, vanc, colistin (KVC) discs when testing is performed on anaerobic organisms?
A
ID + gram stain differentiation
15
Q
T or F. Gram negs are susceptible to colistin
A
T! found in CNA
negs are V resistant
16
Q
lecithinase C reaction
A
- wide zone opacity in the medium which surrounds colonie and always extends beyond their edge
- lecithin is component of egg yolk; split into phosphorylcholine and insoluble diglycerides
17
Q
lipase rxn
A
- irridescent pearly film
- lipase precipitate and paearly layer are composed of fatty acids formed by the breakdown of free fats in egg yolk
18
Q
catalase for anaerobes
A
- should be performed on colonies growing on a medium containing hemin
- better results when colonies are exposed to oxygen thirty mins prior to testing
- 15% hydrogen peroxide instead of 3%
