LAB 11 - Anaerobes Flashcards

1
Q

T or F. Most of the anaerobic bacteria that cause infections are also part of the normal flora of the oral cavity, vagina, and gut

A

T~

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2
Q

T or F. Most common anaerobic infections are exohenous

A

F! endogenous

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3
Q

aerotolerance

A

%
BAP*
CHOC

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4
Q

Actinomyces israelii

A
  • wt/molar tooth
  • branching gpb pleo
  • cat neg
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5
Q

B. fragilis

A
  • shygy, wtgy, gymuc
  • gncb
  • cat pos
  • BBEA +
  • K, V, C resistant
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6
Q

Prevotella sp./Porphyromonas sp.

A
  • brown/black convex; 1-3 wks
  • gnb/cb
  • cat neg
  • BBEA neg
  • young colonies = brick red fluorescence
  • KV resistant
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7
Q

C. perfringens

A
  • DZH
  • gpb, boxcars
  • cat neg
  • reverse camp pos
  • lec pos
  • lipase neg
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8
Q

C. sordellii

A
  • spr/swr
  • gpb, spores
  • cat neg
  • reverse camp neg
  • lecithinase pos
  • lipase neg
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9
Q

C. sporogenes

A
  • tgy/rh (yel center)
  • gpb, spores
  • cat neg
  • reverse CAMP neg
  • lec neg
  • lipase pos
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10
Q

Fusobacterium

A
  • v strict anaerobe
  • bread crumbs, speck, sm
  • gnb, fine, tapered
  • cat neg
  • indole pos
  • UV = chartreuse (pale yel/grn fluorescence)
  • V resistant
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11
Q

Peptostreptococcus sp.

A
  • gy gold, wt op
  • gpc, ch
  • cat neg
  • KC resistant
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12
Q

Cutibacterium acnes

A

(prev. Prop)
- tiny wt/ op
- gpb, pleo (club shaped)
- cat pos
- colistin R

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13
Q

Veillonella

A
  • sm gy
  • sm gnc, sheets
  • cat V
  • nitrate to nitrite
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14
Q

what is the purpose of kanamycin, vanc, colistin (KVC) discs when testing is performed on anaerobic organisms?

A

ID + gram stain differentiation

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15
Q

T or F. Gram negs are susceptible to colistin

A

T! found in CNA
negs are V resistant

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16
Q

lecithinase C reaction

A
  • wide zone opacity in the medium which surrounds colonie and always extends beyond their edge
  • lecithin is component of egg yolk; split into phosphorylcholine and insoluble diglycerides
17
Q

lipase rxn

A
  • irridescent pearly film
  • lipase precipitate and paearly layer are composed of fatty acids formed by the breakdown of free fats in egg yolk
18
Q

catalase for anaerobes

A
  • should be performed on colonies growing on a medium containing hemin
  • better results when colonies are exposed to oxygen thirty mins prior to testing
  • 15% hydrogen peroxide instead of 3%