Lab 3 Molecular Evolution

Question 1

Mammals distinguished 5 basic tastes

Answer 1

• Sweet, sour, bitter, salty, and umami(taste of monosodium glutamate)

Question 2

Test perception is a 2 step process

Answer 2

• molecules nimbus to a specific receptor on the surface of a taste cell
• taste cell generates a nervous impulse interpreted by the brain.

Question 3

Inability to taste PTC is a recessive trait that varies in?

Answer 3

The human population

Question 4

Bitter-tasting compounds are recognized by?

Answer 4

• receptor proteins on the surface of taste cells.

Question 5

Approximately——– for a different bitter taste receptor in mammals

Answer 5

30 genes

Question 6

The gene for PTC taste receptor, TAS2R38 was identified in?

Answer 6

2003

Question 7

variable nucleotide position in human population

Answer 7

• single nucleotide polymorphism( SNP)

Question 8

What type of changes influences the activity of many drugs/

Answer 8

Analogous

Question 9

In this experiment, a sample of a human cell is obtained by?

Answer 9

• Buccal swab

Question 10

DNA is extracted by?

Answer 10

• Boiling with chelex resin, when binding contaminates metal ions.

Question 11

Polymerase chain reaction (PCR) is used to?

Answer 11

• Amplify a short region of the TAS2R38 gene

Question 12

Amplified PCR product is digested with the restriction?

Answer 12

enzyme Haelll

Question 13

Each student scores

Answer 13

• Genotype
• Predicts tasting ability
• Then tastes PTC paper

Question 14

how genotypes frequencies conform to?

Answer 14

Hardy-Weinberg Equilibrium

Question 15

We’re gonna look at

Answer 15

Correlation btw genotype and phenotype. Specifically ptc- bitter compounds

Question 16

Pcr

Answer 16

Technique for rapidly reproducing million to billions of copies form an specific segment of DNA

Question 17

Restrictions enzyme

Answer 17

Generate compatible end of PCR products.

Question 18

Gel electrophoresis

Answer 18

Technique- gel fragments put through a gel matrix by electric current separating DNA by size

Question 19

Accuracy

Answer 19

True value of a given quality

Question 20

precision

Answer 20

Reproducibility of measurements